AB80946

Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6]

Name: Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946) | Abcam
Brand: Abcam Limited
SKU: AB80946
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(23 Publications)

Mouse Monoclonal Superoxide Dismutase 3/EC-SOD antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 23 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human Extracellular superoxide dismutase [Cu-Zn].

View Alternative Names

Extracellular superoxide dismutase [Cu-Zn], EC-SOD, SOD3

3 Images

Flow Cytometry - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (AB80946)

Flow Cyt

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Flow Cytometry - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (AB80946)

Overlay histogram showing HEK293 cells stained with ab80946 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80946, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (AB80946)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (AB80946)

ab80946 at 1/100 dilution staining Superoxide Dismutase 3/EC-SOD in human cartilage by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A HRP-conjugated anti mouse secondary was used at 1/10 dilution. Image 'a' repersents control, 'b' staining of tissue from young human cartilage and 'c' show staining cartilage of a person suffered from osteoarthritis.

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Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (AB80946)

All lanes:

Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (ab80946) at 1 µg/mL

All lanes:

Lung (Mouse) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 26 kDa

Observed band size: 25 kDa,54 kDa

true

Exposure time: 1min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4G11G6

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Mouse, Human, Rat

Applications

IHC-P, WB, Flow Cyt, IHC-Fr, ELISA, ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Human Extracellular superoxide dismutase [Cu-Zn].

P08294

Specificity

Detects extracellular Superoxide Dismutase 3/EC-SOD.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 3 also known as EC-SOD is an antioxidant enzyme with a molecular weight of approximately 135 kDa. It's an extracellular enzyme that catalyzes the dismutation of superoxide radicals into hydrogen peroxide and molecular oxygen. EC-SOD is found mostly in the extracellular matrix tissues such as lungs blood vessels and heart. Its activity depends on the presence of metal ions copper and zinc leading its classification as a Cu/Zn SOD enzyme. Scientists often study EC-SOD using ELISA assays because of its importance in neutralizing reactive oxygen species.

Biological function summary

The enzyme acts as an important regulator of oxidative stress by reducing levels of superoxide radicals. EC-SOD forms a complex with heparan sulfate proteoglycans in the extracellular matrix which influences its stability and distribution. This enzyme plays a role in maintaining the redox balance influencing cell signaling inflammation and tissue damage which makes it important in managing oxidative stress in different tissues including the cardiovascular and respiratory systems.

Pathways

Superoxide dismutase 3 participates in antioxidant pathways important for cellular defense against oxidative stress. It is particularly important in the oxidative stress response pathway. By reducing superoxide radicals EC-SOD influences pathways involving other antioxidant proteins like catalase and glutathione peroxidase. These interactions help prevent the accumulation of harmful oxidants and maintain cellular homeostasis. EC-SOD's action connects closely with other superoxide dismutases such as Cu/Zn SOD and MnSOD within these protective pathways.

Enhanced or impaired EC-SOD function associates with various health issues such as cardiovascular diseases and chronic obstructive pulmonary disease (COPD). EC-SOD has been shown to protect vascular health by reducing oxidative stress related damages which can mitigate the risk of atherosclerosis. In the context of respiratory conditions like COPD alterations in EC-SOD expression can disrupt lung tissue function and inflammatory responses. Its interactions with proteins like catalase further contribute to its protective role against cell and tissue damage during disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.

See full target information Extracellular superoxide dismutase [Cu-Zn]

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Global spine journal :21925682251383489 PubMed41001708

2025

Intervention of Rutin and Ochnaflavone in the Attenuation of Neuroinflammation and Neuronal Apoptosis in Spinal Cord Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Yu,Xuhao Yang,Anyuan Dai,Zhihe Yun,Wu Xue,Tianyang Yuan,Xinyu Nie,Inbo Han,Yanting Liu,A O Wang,Qinyi Liu

Antioxidants (Basel, Switzerland) 14: PubMed40563335

2025

CSE-Induced ER-Mitochondria Crosstalk Promotes Oxidative Stress and Impairs Bronchial Contractile Response.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge Rodríguez-Pérez,Rosa Andreu-Martínez,Leila Pérez-Sánchez,Ana Hernández-García,Cecilia Muñoz-Calleja,Ángel Cogolludo,María J Calzada

Nature neuroscience 28:1446-1459 PubMed40437054

2025

Choroid plexus apocrine secretion shapes CSF proteome during mouse brain development.

Applications

Unspecified application

Species

Unspecified reactive species

Ya'el Courtney,Joshua P Head,Neil Dani,Olga V Chechneva,Frederick B Shipley,Yong Zhang,Michael J Holtzman,Cameron Sadegh,Towia A Libermann,Maria K Lehtinen

Journal of molecular neuroscience : MN 75:38 PubMed40106157

2025

Proteomic Profiling and Therapeutic Targeting of Oxidative Stress in Autoimmune Encephalitis.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Zhang,Cong Zhang,Wei Sun,Shuhua Xie,Pengcheng Wu,Guoyong Zeng,Xianghong Liu

Cell biology and toxicology 41:13 PubMed39707048

2024

Mechanistic insights into sevoflurane-induced hippocampal neuronal damage and cognitive dysfunction through the NEAT1/Nrf2 signaling axis in aged rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yiliang Wang,Nu Li,Xiaoyu Chen,Yue Zhao,Letian Qu,Dasheng Cai

Translational psychiatry 14:415 PubMed39362869

2024

Oral antioxidant edaravone protects against cognitive deficits induced by chronic hypobaric hypoxia at high altitudes.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan-Yuan Ma,Xin Li,Zhong-Yuan Yu,Tong Luo,Cheng-Rong Tan,Yu-Di Bai,Gang Xu,Bin-Da Sun,Xian-Le Bu,Yu-Hui Liu,Wang-Sheng Jin,Yu-Qi Gao,Xin-Fu Zhou,Juan Liu,Yan-Jiang Wang

Biology 13: PubMed39194537

2024

Impact of BMI and Cardiorespiratory Fitness on Oxidative Stress in Plasma and Circulating Exosomes Following Acute Exercise.

Applications

Unspecified application

Species

Unspecified reactive species

Aaron L Slusher,Nishant P Visavadiya,Brandon G Fico,Brisamar Estébanez,Edmund O Acevedo,Chun-Jung Huang

BMC pulmonary medicine 23:385 PubMed37828534

2023

Sequential inspiratory muscle exercise-noninvasive positive pressure ventilation alleviates oxidative stress in COPD by mediating SOCS5/JAK2/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yirou Lei,Jiaying He,Fang Hu,Hao Zhu,Jing Gu,Lijuan Tang,Man Luo

Free radical biology & medicine 193:9-22 PubMed36174878

2022

Cigarette smoke induces pulmonary arterial dysfunction through an imbalance in the redox status of the soluble guanylyl cyclase.

Applications

Unspecified application

Species

Unspecified reactive species

J Sevilla-Montero,O Munar-Rubert,J Pino-Fadón,C Aguilar-Latorre,M Villegas-Esguevillas,B Climent,M Agrò,C Choya-Foces,A Martínez-Ruiz,E Balsa,C Muñoz-Calleja,R M Gómez-Punter,E Vázquez-Espinosa,A Cogolludo,M J Calzada

Neuron 110:3288-3301.e8 PubMed36070751

2022

Choroid plexus-CSF-targeted antioxidant therapy protects the brain from toxicity of cancer chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ahram Jang,Boryana Petrova,Taek-Chin Cheong,Miriam E Zawadzki,Jill K Jones,Andrew J Culhane,Frederick B Shipley,Roberto Chiarle,Eric T Wong,Naama Kanarek,Maria K Lehtinen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6]

Flow Cytometry - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (AB80946)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (AB80946)

Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody [4G11G6] (AB80946)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (23)

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