AB21974

Anti-Superoxide Dismutase 3/EC-SOD antibody

(2 Reviews)

(13 Publications)

Rabbit Polyclonal Superoxide Dismutase 3/EC-SOD antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human Extracellular superoxide dismutase [Cu-Zn] aa 1-50.

View Alternative Names

Extracellular superoxide dismutase [Cu-Zn], EC-SOD, SOD3

3 Images

Immunohistochemistry (Frozen sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody (AB21974)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody (AB21974)

ab21974 (1/1000) staining of ciliated bronchiolar cells in human lung.

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody (AB21974)

ab21974 (1/1000) staining of human kidney tubule cells.

Unknown

Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody (AB21974)

Superoxide Dismutase 3 / EC-SOD contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab21974) at 1/500 dilution

Lane 1:

Lung (Human) Tissue Lysate at 10 µg

Lane 2:

Human kidney tissue lysate - total protein (<a href='/en-us/products/unavailable/human-kidney-tissue-lysate-total-protein-ab30203'>ab30203</a>) at 10 µg

Lane 3:

Human placenta tissue lysate - total protein (<a href='/en-us/products/unavailable/human-placenta-tissue-lysate-total-protein-ab29745'>ab29745</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 26 kDa

Observed band size: 31 kDa,75 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, IHC-Fr

applications

Immunogen

Synthetic Peptide within Human Extracellular superoxide dismutase [Cu-Zn] aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P08294

Specificity

Specific to human extracellular SOD (SOD3). Under reducing conditions the SOD3 antibody shows a major band at approximately 30kDa which is the approximate molecular weight of the SOD3 monomer and two faint minor bands at approximately 50 and 70 kDa. The SOD3 molecule is a homotetramer (4 monomers) with a combined molecular weight of around a 130 Kda. The two faint bands are probably dimers and triplicates of the monomer. This pattern is common with many tetramers (such as cellular glutathione peroxidase) as there is incomplete reduction with DTT.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/4000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/200 - 1/2000", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.02% Thimerosal (merthiolate) Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 3 also known as EC-SOD is an antioxidant enzyme with a molecular weight of approximately 135 kDa. It's an extracellular enzyme that catalyzes the dismutation of superoxide radicals into hydrogen peroxide and molecular oxygen. EC-SOD is found mostly in the extracellular matrix tissues such as lungs blood vessels and heart. Its activity depends on the presence of metal ions copper and zinc leading its classification as a Cu/Zn SOD enzyme. Scientists often study EC-SOD using ELISA assays because of its importance in neutralizing reactive oxygen species.

Biological function summary

The enzyme acts as an important regulator of oxidative stress by reducing levels of superoxide radicals. EC-SOD forms a complex with heparan sulfate proteoglycans in the extracellular matrix which influences its stability and distribution. This enzyme plays a role in maintaining the redox balance influencing cell signaling inflammation and tissue damage which makes it important in managing oxidative stress in different tissues including the cardiovascular and respiratory systems.

Pathways

Superoxide dismutase 3 participates in antioxidant pathways important for cellular defense against oxidative stress. It is particularly important in the oxidative stress response pathway. By reducing superoxide radicals EC-SOD influences pathways involving other antioxidant proteins like catalase and glutathione peroxidase. These interactions help prevent the accumulation of harmful oxidants and maintain cellular homeostasis. EC-SOD's action connects closely with other superoxide dismutases such as Cu/Zn SOD and MnSOD within these protective pathways.

Enhanced or impaired EC-SOD function associates with various health issues such as cardiovascular diseases and chronic obstructive pulmonary disease (COPD). EC-SOD has been shown to protect vascular health by reducing oxidative stress related damages which can mitigate the risk of atherosclerosis. In the context of respiratory conditions like COPD alterations in EC-SOD expression can disrupt lung tissue function and inflammatory responses. Its interactions with proteins like catalase further contribute to its protective role against cell and tissue damage during disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.

See full target information Extracellular superoxide dismutase [Cu-Zn]

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

The FEBS journal 292:2624-2644 PubMed39957045

2025

HDAC6 inhibition upregulates endothelial SOD3 expression via Sp1 acetylation and attenuates angiotensin II-induced hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Zhexi Chi,Van Quan Do,Rukhsana Kausar,Hyun Kyung Kim,Nga Thi Thanh Nguyen,Truc Phan Hoang Le,Jungwoo Lee,In-Jeoung Baek,Sang-Wook Lee,Jae Hyung Kim,Sang Yoon Lee

Stem cell research & therapy 12:142 PubMed33596998

2021

Differential SOD2 and GSTZ1 profiles contribute to contrasting dental pulp stem cell susceptibilities to oxidative damage and premature senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Nadia Y A Alaidaroos,Amr Alraies,Rachel J Waddington,Alastair J Sloan,Ryan Moseley

PloS one 13:e0202501 PubMed30125327

2018

Dysregulated expression of antioxidant enzymes in polyethylene particle-induced periprosthetic inflammation and osteolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Kuo-Ti Peng,Meng-Hsueh Tsai,Chiang-Wen Lee,Yao-Chang Chiang,Pei-Chun Chen,Chun-Chieh Chen,Chih-Hsiang Chang,Hsin-Nung Shih,Pey-Jium Chang

Molecules and cells 39:242-9 PubMed26912083

2016

Cloning, Purification, and Characterization of Recombinant Human Extracellular Superoxide Dismutase in SF9 Insect Cells.

Applications

Species

Unspecified reactive species

Pravesh Shrestha,Ji-Hye Yun,Woo Taek Kim,Tae-Yoon Kim,Weontae Lee

American journal of physiology. Regulatory, integr 308:R945-56 PubMed25810384

2015

Pregnant rats treated with a high-fat/prooxidant Western diet with ANG II and TNF-α are resistant to elevations in blood pressure and renal oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Mark W Cunningham,Crystal A West,Xuerong Wen,Aihua Deng,Chris Baylis

Scientific reports 4:7144 PubMed25413454

2014

Aging disrupts cell subpopulation dynamics and diminishes the function of mesenchymal stem cells.

Applications

IHC-Fr

Species

Mouse

Dominik Duscher,Robert C Rennert,Michael Januszyk,Ersilia Anghel,Zeshaan N Maan,Alexander J Whittam,Marcelina G Perez,Revanth Kosaraju,Michael S Hu,Graham G Walmsley,David Atashroo,Sacha Khong,Atul J Butte,Geoffrey C Gurtner

American journal of hypertension 26:1242-9 PubMed23764378

2013

Renal nitric oxide synthase and antioxidant preservation in Cyp1a1-Ren-2 transgenic rats with inducible malignant hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Mark W Cunningham,Jennifer M Sasser,Crystal A West,Carlo J Milani,Chris Baylis,Kenneth D Mitchell

Life sciences 91:54-63 PubMed22727796

2012

Nebivolol does not protect against 5/6 ablation/infarction induced chronic kidney disease in rats - comparison with angiotensin II receptor blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer M Sasser,Natasha C Moningka,Tatsiana Tsarova,Chris Baylis

American journal of physiology. Gastrointestinal and liver physiology 302:G182-93 PubMed21979757

2011

Suppression of the HPA axis during extrahepatic biliary obstruction induces cholangiocyte proliferation in the rat.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Quinn,Yoshiyuki Ueno,Hae Yong Pae,Li Huang,Gabriel Frampton,Cheryl Galindo,Heather Francis,Darijana Horvat,Matthew McMillin,Sharon Demorrow

The Journal of physiology 589:6129-38 PubMed21969451

2011

Twelve weeks of treadmill exercise does not alter age-dependent chronic kidney disease in the Fisher 344 male rat.

Applications

Species

Rat

Natasha C Moningka,Amy L Sindler,Judy M Muller-Delp,Chris Baylis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Superoxide Dismutase 3/EC-SOD antibody

Immunohistochemistry (Frozen sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody (AB21974)

Immunohistochemistry (Frozen sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody (AB21974)

Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody (AB21974)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise