AB83108

Anti-Superoxide Dismutase 3/EC-SOD antibody

Name: Anti-Superoxide Dismutase 3/EC-SOD antibody (ab83108) | Abcam
Brand: Abcam Limited
SKU: AB83108
Price: 535 USD
Availability: InStock

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(15 Publications)

Rabbit Polyclonal Superoxide Dismutase 3/EC-SOD antibody. Suitable for WB and reacts with Rat, Mouse, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human Extracellular superoxide dismutase [Cu-Zn].

View Alternative Names

Extracellular superoxide dismutase [Cu-Zn], EC-SOD, SOD3

1 Images

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Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody (AB83108)

All lanes:

Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody (ab83108) at 1/1000 dilution

Lane 1:

Rat lung tissue extract

Lane 2:

Mouse lung tissue extract

Lane 3:

Human Superoxide Dismutase 3/EC-SOD protein

Predicted band size: 26 kDa

Observed band size: 35 kDa,40 kDa,60 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Synthetic Peptide within Human Extracellular superoxide dismutase [Cu-Zn]. The exact immunogen used to generate this antibody is proprietary information.

P08294

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 3 also known as EC-SOD is an antioxidant enzyme with a molecular weight of approximately 135 kDa. It's an extracellular enzyme that catalyzes the dismutation of superoxide radicals into hydrogen peroxide and molecular oxygen. EC-SOD is found mostly in the extracellular matrix tissues such as lungs blood vessels and heart. Its activity depends on the presence of metal ions copper and zinc leading its classification as a Cu/Zn SOD enzyme. Scientists often study EC-SOD using ELISA assays because of its importance in neutralizing reactive oxygen species.

Biological function summary

The enzyme acts as an important regulator of oxidative stress by reducing levels of superoxide radicals. EC-SOD forms a complex with heparan sulfate proteoglycans in the extracellular matrix which influences its stability and distribution. This enzyme plays a role in maintaining the redox balance influencing cell signaling inflammation and tissue damage which makes it important in managing oxidative stress in different tissues including the cardiovascular and respiratory systems.

Pathways

Superoxide dismutase 3 participates in antioxidant pathways important for cellular defense against oxidative stress. It is particularly important in the oxidative stress response pathway. By reducing superoxide radicals EC-SOD influences pathways involving other antioxidant proteins like catalase and glutathione peroxidase. These interactions help prevent the accumulation of harmful oxidants and maintain cellular homeostasis. EC-SOD's action connects closely with other superoxide dismutases such as Cu/Zn SOD and MnSOD within these protective pathways.

Enhanced or impaired EC-SOD function associates with various health issues such as cardiovascular diseases and chronic obstructive pulmonary disease (COPD). EC-SOD has been shown to protect vascular health by reducing oxidative stress related damages which can mitigate the risk of atherosclerosis. In the context of respiratory conditions like COPD alterations in EC-SOD expression can disrupt lung tissue function and inflammatory responses. Its interactions with proteins like catalase further contribute to its protective role against cell and tissue damage during disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.

See full target information Extracellular superoxide dismutase [Cu-Zn]

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Medical gas research 15:383-390 PubMed39923134

2025

Variability in flow-induced vasodilation mechanisms in cerebral arteries: the impact of different hyperbaric oxygen protocols.

Applications

Unspecified application

Species

Unspecified reactive species

Ines Drenjančević,Ivana Jukić,Vedran Đambić,Ana Stupin,Nataša Kozina,Anita Matić,Petar Šušnjara,Aleksandar Kibel,Darko Biljan,Zrinka Mihaljević

Frontiers in immunology 15:1367432 PubMed38994364

2024

The mammosphere-derived epithelial cell secretome modulates neutrophil functions in the bovine model.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca M Harman,Anja Sipka,Kelly A Oxford,Leane Oliveira,Lucas Huntimer,Daryl V Nydam,Gerlinde R Van de Walle

BMC complementary medicine and therapies 23:131 PubMed37098557

2023

Sodium selenite preserves rBM-MSCs' stemness, differentiation potential, and immunophenotype and protects them against oxidative stress via activation of the Nrf2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bahareh Rahimi,Mohammad Panahi,Hajie Lotfi,Mostafa Khalili,Astireh Salehi,Neda Saraygord-Afshari,Effat Alizadeh

Nutrients 14: PubMed35631204

2022

Effects of Iron Supplementation on Testicular Function and Spermatogenesis of Iron-Deficient Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Wei Tsao,Yuan-Ru Liao,Ting-Chia Chang,Yih-Fong Liew,Chin-Yu Liu

Antioxidants (Basel, Switzerland) 11: PubMed35624792

2022

SOD3 Suppresses the Expression of MMP-1 and Increases the Integrity of Extracellular Matrix in Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Hyung Kim,Hae Dong Jeong,Min Ji Song,Dong Hun Lee,Jin Ho Chung,Seung-Taek Lee

Experimental and therapeutic medicine 23:157 PubMed35069838

2022

APPL1 ameliorates myocardial ischemia-reperfusion injury by regulating the AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunguang Cen,Wei Liao,Taihao Wang,Daimin Zhang

PloS one 16:e0259597 PubMed34735539

2021

Reduced SOD2 expression does not influence prion disease course or pathology in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Simote T Foliaki,Brent Race,Katie Williams,Chase Baune,Bradley R Groveman,Cathryn L Haigh

Antioxidants (Basel, Switzerland) 10: PubMed34679728

2021

Modulation of SOD3 Levels Is Detrimental to Retinal Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Larissa Ikelle,Muna I Naash,Muayyad R Al-Ubaidi

Acta physiologica (Oxford, England) 233:e13700 PubMed34089562

2021

High-intensity exercise in hypoxia improves endothelial function via increased nitric oxide bioavailability in C57BL/6 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Lavier,Manon Beaumann,Steeve Menétrey,Karima Bouzourène,Nathalie Rosenblatt-Velin,Vincent Pialoux,Lucia Mazzolai,Anne-Christine Peyter,Maxime Pellegrin,Grégoire P Millet

International journal of molecular sciences 22: PubMed34207085

2021

Anakinra Activates Superoxide Dismutase 2 to Mitigate Inflammasome Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Marilena Pariano,Stefania Pieroni,Antonella De Luca,Rossana G Iannitti,Monica Borghi,Matteo Puccetti,Stefano Giovagnoli,Giorgia Renga,Fiorella D'Onofrio,Marina M Bellet,Claudia Stincardini,Maria Agnese Della-Fazia,Giuseppe Servillo,Frank L van de Veerdonk,Claudio Costantini,Luigina Romani

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Superoxide Dismutase 3/EC-SOD antibody

Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody (AB83108)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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