AB249643

Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Superoxide Dismutase 3/EC-SOD antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

Extracellular superoxide dismutase [Cu-Zn], EC-SOD, SOD3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] - BSA and Azide free (AB249643)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] - BSA and Azide free (AB249643)

This data was developed using ab171738, the same antibody clone in a different buffer formulation.

Immunohistochemistry of paraffin-embedded Human heart tissue labeling Superoxide Dismutase 3/EC-SOD using ab171738 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] - BSA and Azide free (AB249643)

This data was developed using ab171738, the same antibody clone in a different buffer formulation.

Immunohistochemistry of paraffin-embedded Human kidney tissue labeling Superoxide Dismutase 3/EC-SOD using ab171738 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] - BSA and Azide free (AB249643)

This data was developed using ab171738, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] (<a href='/en-us/products/primary-antibodies/superoxide-dismutase-3-ec-sod-antibody-epr10860-ab171738'>ab171738</a>) at 1/1000 dilution

Lane 1:

Human fetal lung lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10860

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab249643 is the carrier-free version of ab171738.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 3 also known as EC-SOD is an antioxidant enzyme with a molecular weight of approximately 135 kDa. It's an extracellular enzyme that catalyzes the dismutation of superoxide radicals into hydrogen peroxide and molecular oxygen. EC-SOD is found mostly in the extracellular matrix tissues such as lungs blood vessels and heart. Its activity depends on the presence of metal ions copper and zinc leading its classification as a Cu/Zn SOD enzyme. Scientists often study EC-SOD using ELISA assays because of its importance in neutralizing reactive oxygen species.

Biological function summary

The enzyme acts as an important regulator of oxidative stress by reducing levels of superoxide radicals. EC-SOD forms a complex with heparan sulfate proteoglycans in the extracellular matrix which influences its stability and distribution. This enzyme plays a role in maintaining the redox balance influencing cell signaling inflammation and tissue damage which makes it important in managing oxidative stress in different tissues including the cardiovascular and respiratory systems.

Pathways

Superoxide dismutase 3 participates in antioxidant pathways important for cellular defense against oxidative stress. It is particularly important in the oxidative stress response pathway. By reducing superoxide radicals EC-SOD influences pathways involving other antioxidant proteins like catalase and glutathione peroxidase. These interactions help prevent the accumulation of harmful oxidants and maintain cellular homeostasis. EC-SOD's action connects closely with other superoxide dismutases such as Cu/Zn SOD and MnSOD within these protective pathways.

Enhanced or impaired EC-SOD function associates with various health issues such as cardiovascular diseases and chronic obstructive pulmonary disease (COPD). EC-SOD has been shown to protect vascular health by reducing oxidative stress related damages which can mitigate the risk of atherosclerosis. In the context of respiratory conditions like COPD alterations in EC-SOD expression can disrupt lung tissue function and inflammatory responses. Its interactions with proteins like catalase further contribute to its protective role against cell and tissue damage during disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.

See full target information Extracellular superoxide dismutase [Cu-Zn]

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] - BSA and Azide free (AB249643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] - BSA and Azide free (AB249643)

Western blot - Anti-Superoxide Dismutase 3/EC-SOD antibody [EPR10860] - BSA and Azide free (AB249643)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise