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AB137131

Anti-Superoxide Dismutase 4/CCS antibody [EPR9713(B)]

2

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal Superoxide Dismutase 4/CCS antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Copper chaperone for superoxide dismutase, Superoxide dismutase copper chaperone, CCS

1 Images
Western blot - Anti-Superoxide Dismutase 4/CCS antibody [EPR9713(B)] (AB137131)
  • WB

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Western blot - Anti-Superoxide Dismutase 4/CCS antibody [EPR9713(B)] (AB137131)

All lanes:

Western blot - Anti-Superoxide Dismutase 4/CCS antibody [EPR9713(B)] (ab137131) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 30 kDa

false

  • Carrier free

    Anti-Superoxide Dismutase 4/CCS antibody [EPR9713(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9713(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 4 also known as CCS or Copper Chaperone for SOD1 plays a mechanical role as a copper chaperone for the antioxidant enzyme SOD1 (Superoxide Dismutase 1). With a molecular mass of approximately 30 kDa CCS assists in inserting copper into SOD1 activating it for its enzymatic function. CCS is expressed in various tissues including the brain liver and muscle facilitating its role wherever SOD1 activity is needed for protection against oxidative damage.
Biological function summary

CCS contributes significantly to oxidative stress response by catalyzing the dismutation of superoxide radicals into less reactive molecular oxygen and hydrogen peroxide. This protein does not function independently but is part of a complex system involving SOD1 to mitigate oxidative damage within cells. CCS ensures SOD1 acquires the necessary copper which is essential for its dismutase activity hence playing a critical role in maintaining cellular health.

Pathways

The copper delivery to SOD1 by CCS integrates into the broader antioxidant defense pathway. The copper chaperones like CCS link to pathways involving cellular copper homeostasis and oxidative stress management. The protein SOD1 which relies on CCS for copper addition also aligns with the apoptosis and neuroprotective pathways highlighting a shared role in cellular oxidation states control and maintaining neuronal integrity.

CCS associates with conditions such as Amyotrophic Lateral Sclerosis (ALS) and certain neurodegenerative disorders. These conditions relate to the malfunction of the antioxidant defense often involving misfolded SOD1 due to improper copper loading. The impairment in CCS’s role affects not just SOD1 but potentially other copper-dependent proteins exacerbating stress conditions and cellular vulnerability that characterizes these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Delivers copper to copper zinc superoxide dismutase (SOD1).
See full target information CCS
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 37:101746 PubMed33059313

2020

Copper interferes with selenoprotein synthesis and activity.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Schwarz,Kristina Lossow,Katja Schirl,Julian Hackler,Kostja Renko,Johannes Florian Kopp,Tanja Schwerdtle,Lutz Schomburg,Anna Patricia Kipp
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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