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AB254629

Anti-Supervillin antibody

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(1 Publication)

Rabbit Polyclonal Supervillin antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SVIL aa 800-1000.

View Alternative Names

Supervillin, Archvillin, p205/p250, SVIL

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Supervillin antibody (AB254629)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Supervillin antibody (AB254629)

Paraffin-embedded human skeletal muscle tissue stained for Supervillin using ab254629 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Supervillin antibody (AB254629)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Supervillin antibody (AB254629)

Paraffin-embedded human prostate tissue stained for Supervillin using ab254629 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Supervillin antibody (AB254629)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Supervillin antibody (AB254629)

PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling Supervillin using ab254629 at 4µg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Supervillin antibody (AB254629)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Supervillin antibody (AB254629)

Paraffin-embedded human heart tissue stained for Supervillin using ab254629 at 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SVIL aa 800-1000. The exact immunogen used to generate this antibody is proprietary information.

O95425

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Supervillin also known as SVIL is a multifunctional protein with a molecular mass of approximately 205 kDa. It plays a significant role in cytoskeletal dynamics by acting as a scaffolding protein connecting actin filaments. This connection facilitates the modulation of cellular structure and motility. The protein is ubiquitously expressed with high levels found in muscle endothelial and certain epithelial cells. Supervillin has fundamental elements such as multiple ankyrin repeat domains sequences resembling gelsolin and villin headpieces which aid in its multifunctionality.
Biological function summary

Supervillin contributes to the formation and organization of cytoskeletal components. It exists as part of a larger protein complex that regulates actin filament assembly and stabilization. This protein influences cell adhesion migration and possibly axonal growth by providing a structural framework and regulatory interface. Its interaction with myosin II further suggests a role in cellular contraction events adding another layer of complexity to its biological function.

Pathways

Supervillin integrates into important cellular processes such as the regulation of the actin cytoskeleton and cell adhesion pathways. It operates in conjunction with other proteins like integrins contributing to focal adhesion dynamics. Another key pathway involves RhoA signaling where Supervillin participates in modulating cell shape and motility. It collaborates with proteins in the Rho family to control actin filament rearrangements vital for cell movement and tissue architecture.

Disruptions in Supervillin function link to muscular dystrophies and certain types of cancer. Altered expression levels can influence the invasive potential of cancer cells and affect tumor progression through interactions with proteins like integrins and other components of adhesion complexes. In muscular disorders the relationship with myosin II highlights a connection to muscle contraction abnormalities contributing to disease pathology. These associations emphasize the importance of Supervillin in maintaining cellular and tissue integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. Forms a high-affinity link between the actin cytoskeleton and the membrane. Is among the first costameric proteins to assemble during myogenesis and it contributes to myogenic membrane structure and differentiation (PubMed : 12711699). Appears to be involved in myosin II assembly. May modulate myosin II regulation through MLCK during cell spreading, an initial step in cell migration. May play a role in invadopodial function (PubMed : 19109420).. Isoform 2. May be involved in modulation of focal adhesions. Supervillin-mediated down-regulation of focal adhesions involves binding to TRIP6. Plays a role in cytokinesis through KIF14 interaction (By similarity).
See full target information SVIL
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 6: PubMed37550000

2023

Macrophage promotes pathological neovascularization in age-related macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yafang Wang,Yang Liu,Yan Wang,Yidong Wu,Zhixuan Chen,Feng Wang,Xiaoling Wan,Fenghua Wang,Xiaodong Sun
View all publications
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Product promise

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