Rabbit Polyclonal SUPT16H antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SUPT16H aa 150-300.
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Publications
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- International journal of molecular sciences 24:2023Haploinsufficiency Impairs PI3K/AKT/mTOR/Autophagy Pathway in Human Pluripotent Stem Cells Derived Neural Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunwen Wang,Ziyi Wang,Limeng Dai,Xintong Zhu,Xingying Guan,Junyi Wang,Jia Li,Mao Zhang,Yun Bai,Hong GuoPubMed 36769360
- eLife 11:2022The TFIIH complex is required to establish and maintain mitotic chromosome structure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulian Haase,Richard Chen,Wesley M Parker,Mary Kate Bonner,Lisa M Jenkins,Alexander E KellyPubMed 35293859
- Molecular cancer therapeutics 19:258-2692019Targeting Histone Chaperone FACT Complex Overcomes 5-Fluorouracil Resistance in Colon Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeyu Song,Jiping Zeng,Shrabasti Roychoudhury,Pranjal Biswas,Bhopal Mohapatra,Sutapa Ray,Kayvon Dowlatshahi,Jing Wang,Vimla Band,Geoffrey Talmon,Kishor K BhakatPubMed 31575655
- Human molecular genetics 24:6565-792015A role for repressive complexes and H3K9 di-methylation in PRDM5-associated brittle cornea syndrome.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SUPT16H aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5B9
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/2500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Xenopus laevis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Xenopus laevis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Xenopus laevis | Dilution info - | Notes - |
Associated Products
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Target data
Function
Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II).
Alternative names
FACT140, FACTP140, SUPT16H, FACT complex subunit SPT16, Chromatin-specific transcription elongation factor 140 kDa subunit, FACT 140 kDa subunit, FACTp140, Facilitates chromatin transcription complex subunit SPT16, hSPT16
Recommended products
Rabbit Polyclonal SUPT16H antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SUPT16H aa 150-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SUPT16H aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5B9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SUPT16H also known as FACT80 is a protein that plays an important mechanical role in chromatin remodeling. It has a molecular mass of approximately 142 kDa. This protein becomes substantially expressed in actively dividing cells particularly in tissues with high rates of cell proliferation such as the bone marrow and epithelial layers. SUPT16H along with SSRP1 forms a heterodimeric structure that functions as the Facilitates Chromatin Transcription (FACT) complex which modulates nucleosome organization during transcription elongation.
- Biological function summary
The SUPT16H protein operates by working within the FACT complex to unravel nucleosomes allowing RNA polymerase II to move along DNA more efficiently by transiently modifying chromatin structure. This function is essential during the transcriptional elongation process making it pivotal in gene expression regulation. The protein as part of the FACT complex participates in chromatin destabilization and reassembly proving important in maintaining chromatin integrity during cell cycle progression and other cellular stress responses.
- Pathways
SUPT16H plays a role in the transcriptional regulation pathway. It is strongly linked to pathways like the p53 signaling pathway which responds to DNA damage and maintains genomic stability. Within these pathways SUPT16H interacts with proteins such as RNA polymerase II highlighting its role in transcriptional regulation. In direct relation to this function SUPT16H also connects with p53-related proteins modulating their transcriptional activity in response to cellular changes.
- Associated diseases and disorders
The SUPT16H protein has demonstrated involvement in various cancer types due to its role in gene expression regulation. Aberrations in its function can lead to dysregulated transcription contributing to oncogenic transformations. Furthermore SUPT16H ties into neurodegenerative disorders such as Alzheimer's disease where altered chromatin dynamics may impact neuronal survival. In these contexts SUPT16H associates with cancer-related proteins and those involved in neuronal signaling pathways highlighting its importance in disease pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUPT16H antibody (ab204343)
Immunohistochemical analysis of paraffin-embedded Human rectum tissue labeling SUPT16H with ab204343 at 1/1000 dilution.
Western blot - Anti-SUPT16H antibody (ab204343)
All lanes: Western blot - Anti-SUPT16H antibody (ab204343) at 1/500 dilution
Lane 1: RT-4 cell lysate
Lane 2: U-251 MG cell lysate
Lane 3: Human plasma
Lane 4: Human liver tissue lysate
Lane 5: Human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 120 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SUPT16H antibody (ab204343)
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling SUPT16H with ab204343 at 4 μg/ml (green).
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