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AB247721

Anti-SUPT16H antibody [EPR3685] - BSA and Azide free

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Rabbit Recombinant Monoclonal SUPT16H antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

FACT140, FACTP140, SUPT16H, FACT complex subunit SPT16, Chromatin-specific transcription elongation factor 140 kDa subunit, FACT 140 kDa subunit, FACTp140, Facilitates chromatin transcription complex subunit SPT16, hSPT16

1 Images
Western blot - Anti-SUPT16H antibody [EPR3685] - BSA and Azide free (AB247721)
  • WB

Unknown

Western blot - Anti-SUPT16H antibody [EPR3685] - BSA and Azide free (AB247721)

This data was developed using ab108960, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SUPT16H antibody [EPR3685] (<a href='/en-us/products/primary-antibodies/supt16h-antibody-epr3685-ab108960'>ab108960</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Predicted band size: 120 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3685

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab247721 is the carrier-free version of ab108960.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SUPT16H also known as FACT80 is a protein that plays an important mechanical role in chromatin remodeling. It has a molecular mass of approximately 142 kDa. This protein becomes substantially expressed in actively dividing cells particularly in tissues with high rates of cell proliferation such as the bone marrow and epithelial layers. SUPT16H along with SSRP1 forms a heterodimeric structure that functions as the Facilitates Chromatin Transcription (FACT) complex which modulates nucleosome organization during transcription elongation.
Biological function summary

The SUPT16H protein operates by working within the FACT complex to unravel nucleosomes allowing RNA polymerase II to move along DNA more efficiently by transiently modifying chromatin structure. This function is essential during the transcriptional elongation process making it pivotal in gene expression regulation. The protein as part of the FACT complex participates in chromatin destabilization and reassembly proving important in maintaining chromatin integrity during cell cycle progression and other cellular stress responses.

Pathways

SUPT16H plays a role in the transcriptional regulation pathway. It is strongly linked to pathways like the p53 signaling pathway which responds to DNA damage and maintains genomic stability. Within these pathways SUPT16H interacts with proteins such as RNA polymerase II highlighting its role in transcriptional regulation. In direct relation to this function SUPT16H also connects with p53-related proteins modulating their transcriptional activity in response to cellular changes.

The SUPT16H protein has demonstrated involvement in various cancer types due to its role in gene expression regulation. Aberrations in its function can lead to dysregulated transcription contributing to oncogenic transformations. Furthermore SUPT16H ties into neurodegenerative disorders such as Alzheimer's disease where altered chromatin dynamics may impact neuronal survival. In these contexts SUPT16H associates with cancer-related proteins and those involved in neuronal signaling pathways highlighting its importance in disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II).
See full target information SUPT16H
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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