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Rabbit Recombinant Monoclonal SUPT4H antibody. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-SUPT4H antibody [EPR6100(2)] (AB129187), expandable thumbnail
  • OI-RD Scanning - Anti-SUPT4H antibody [EPR6100(2)] (AB129187), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Predicted
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Predicted
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal SUPT4H antibody. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR6100(2)
Purification technique
Affinity purification Protein A
Dissociation constant
1.69 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SUPT4H also known as Suppressor of Ty 4 Homolog or SPT4 Homolog functions as part of the transcription machinery. It forms a complex known as DSIF which involves SUPT4H and a partner protein called SPT5. SUPT4H modulates RNA polymerase II (RNAPII) by forming a complex with SPT5 and facilitating transcription elongation. The molecular mass of SUPT4H is approximately 15 kDa. Expression of SUPT4H occurs broadly across various tissues indicating its essential role in general transcriptional regulation.

Biological function summary

SUPT4H plays an important role in regulating gene expression through its involvement in the DSIF complex. The DSIF complex acts as a transcription factor that associates with paused polymerase influencing transcription elongation. SUPT4H's interaction with SPT5 is important for maintaining the DSIF complex stability and function. SUPT4H and its associated complex help ensure that transcription proceeds efficiently allowing proper gene expression and cellular function.

Pathways

The DSIF complex containing SUPT4H is integral to several critical gene regulatory networks. It is involved in transcriptional pausing and control within the immediate early gene response pathway interfacing with various transcription factors and pause-release factors. Moreover the interaction with NELF (Negative Elongation Factor) provides a checkpoint control in the regulation of RNA polymerase II pausing. These interactions synchronize transcription with RNA processing and output maintaining cellular homeostasis.

Associated diseases and disorders

SUPT4H's role in transcription regulation suggests implications in cancer and neurodegenerative disorders. Altered expression and function can disrupt normal gene expression potentially leading to oncogenesis. In the context of neurodegenerative disorders SUPT4H's regulatory activity might affect neuronal gene expression implicating it in the pathogenesis of diseases such as Alzheimer's. Its interaction with p53-responsive elements also indicates its influence on tumor suppressor gene networks linking it to cancer biology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-SUPT4H antibody [EPR6100(2)] (ab129187), expandable thumbnail

    Western blot - Anti-SUPT4H antibody [EPR6100(2)] (ab129187)

    All lanes: Western blot - Anti-SUPT4H antibody [EPR6100(2)] (ab129187) at 1/1000 dilution

    Lane 1: 293T cell lysates at 10 µg

    Lane 2: MCF-7 cell lysates at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 13 kDa

  • OI-RD Scanning - Anti-SUPT4H antibody [EPR6100(2)] (ab129187), expandable thumbnail

    OI-RD Scanning - Anti-SUPT4H antibody [EPR6100(2)] (ab129187)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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