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AB129187

Anti-SUPT4H antibody [EPR6100(2)]

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(1 Publication)

Rabbit Recombinant Monoclonal SUPT4H antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SPT4H, SUPT4H, SUPT4H1, Transcription elongation factor SPT4, hSPT4, DRB sensitivity-inducing factor 14 kDa subunit, DRB sensitivity-inducing factor small subunit, DSIF p14, DSIF small subunit

2 Images
Western blot - Anti-SUPT4H antibody [EPR6100(2)] (AB129187)
  • WB

Unknown

Western blot - Anti-SUPT4H antibody [EPR6100(2)] (AB129187)

All lanes:

Western blot - Anti-SUPT4H antibody [EPR6100(2)] (ab129187) at 1/1000 dilution

Lane 1:

293T cell lysates at 10 µg

Lane 2:

MCF-7 cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 13 kDa

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OI-RD Scanning - Anti-SUPT4H antibody [EPR6100(2)] (AB129187)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SUPT4H antibody [EPR6100(2)] (AB129187)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-SUPT4H antibody [EPR6100(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6100(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SUPT4H also known as Suppressor of Ty 4 Homolog or SPT4 Homolog functions as part of the transcription machinery. It forms a complex known as DSIF which involves SUPT4H and a partner protein called SPT5. SUPT4H modulates RNA polymerase II (RNAPII) by forming a complex with SPT5 and facilitating transcription elongation. The molecular mass of SUPT4H is approximately 15 kDa. Expression of SUPT4H occurs broadly across various tissues indicating its essential role in general transcriptional regulation.
Biological function summary

SUPT4H plays an important role in regulating gene expression through its involvement in the DSIF complex. The DSIF complex acts as a transcription factor that associates with paused polymerase influencing transcription elongation. SUPT4H's interaction with SPT5 is important for maintaining the DSIF complex stability and function. SUPT4H and its associated complex help ensure that transcription proceeds efficiently allowing proper gene expression and cellular function.

Pathways

The DSIF complex containing SUPT4H is integral to several critical gene regulatory networks. It is involved in transcriptional pausing and control within the immediate early gene response pathway interfacing with various transcription factors and pause-release factors. Moreover the interaction with NELF (Negative Elongation Factor) provides a checkpoint control in the regulation of RNA polymerase II pausing. These interactions synchronize transcription with RNA processing and output maintaining cellular homeostasis.

SUPT4H's role in transcription regulation suggests implications in cancer and neurodegenerative disorders. Altered expression and function can disrupt normal gene expression potentially leading to oncogenesis. In the context of neurodegenerative disorders SUPT4H's regulatory activity might affect neuronal gene expression implicating it in the pathogenesis of diseases such as Alzheimer's. Its interaction with p53-responsive elements also indicates its influence on tumor suppressor gene networks linking it to cancer biology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II (PubMed : 10075709, PubMed : 10199401, PubMed : 10454543, PubMed : 10912001, PubMed : 11112772, PubMed : 11553615, PubMed : 12653964, PubMed : 12718890, PubMed : 15136722, PubMed : 15380072, PubMed : 9450929, PubMed : 9857195). DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A (PubMed : 15136722). DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter (PubMed : 10199401, PubMed : 10912001, PubMed : 11112772). Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex (PubMed : 10199401, PubMed : 10912001, PubMed : 11112772). DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II (PubMed : 10199401, PubMed : 10912001, PubMed : 11112772). TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme (PubMed : 16214896). Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites (PubMed : 16214896). DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat (PubMed : 11112772). DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences (PubMed : 11112772).
See full target information SUPT4H1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Allergologia et immunopathologia 52:46-52 PubMed38970264

2024

Knockdown of GNL3 inhibits LUAD cell growth by regulating Wnt-β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guihong Dai,Yuejun Sun
View all publications
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Product promise

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