Rabbit Recombinant Monoclonal SUPT4H antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-SUPT4H antibody [EPR6100(2)] - BSA and Azide free (AB248339), expandable thumbnail](https://content.abcam.com/products/images/supt4h-antibody-epr61002-bsa-and-azide-free-ab248339--western-blot-img104627.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SUPT4H antibody [EPR6100(2)] - BSA and Azide free (AB248339)")
![OI-RD Scanning - Anti-SUPT4H antibody [EPR6100(2)] - BSA and Azide free (AB248339), expandable thumbnail](https://content.abcam.com/products/images/supt4h-antibody-epr61002-bsa-and-azide-free-ab248339--oi-rd-scanning-img83719.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-SUPT4H antibody [EPR6100(2)] - BSA and Azide free (AB248339)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Predicted | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Predicted | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
Alternative names
SPT4H, SUPT4H, SUPT4H1, Transcription elongation factor SPT4, hSPT4, DRB sensitivity-inducing factor 14 kDa subunit, DRB sensitivity-inducing factor small subunit, DSIF p14, DSIF small subunit
Recommended products
Rabbit Recombinant Monoclonal SUPT4H antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6100(2)
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.69 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248339 is the carrier-free version of Anti-SUPT4H antibody [EPR6100(2)] ab129187.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SUPT4H also known as Suppressor of Ty 4 Homolog or SPT4 Homolog functions as part of the transcription machinery. It forms a complex known as DSIF which involves SUPT4H and a partner protein called SPT5. SUPT4H modulates RNA polymerase II (RNAPII) by forming a complex with SPT5 and facilitating transcription elongation. The molecular mass of SUPT4H is approximately 15 kDa. Expression of SUPT4H occurs broadly across various tissues indicating its essential role in general transcriptional regulation.
- Biological function summary
SUPT4H plays an important role in regulating gene expression through its involvement in the DSIF complex. The DSIF complex acts as a transcription factor that associates with paused polymerase influencing transcription elongation. SUPT4H's interaction with SPT5 is important for maintaining the DSIF complex stability and function. SUPT4H and its associated complex help ensure that transcription proceeds efficiently allowing proper gene expression and cellular function.
- Pathways
The DSIF complex containing SUPT4H is integral to several critical gene regulatory networks. It is involved in transcriptional pausing and control within the immediate early gene response pathway interfacing with various transcription factors and pause-release factors. Moreover the interaction with NELF (Negative Elongation Factor) provides a checkpoint control in the regulation of RNA polymerase II pausing. These interactions synchronize transcription with RNA processing and output maintaining cellular homeostasis.
- Associated diseases and disorders
SUPT4H's role in transcription regulation suggests implications in cancer and neurodegenerative disorders. Altered expression and function can disrupt normal gene expression potentially leading to oncogenesis. In the context of neurodegenerative disorders SUPT4H's regulatory activity might affect neuronal gene expression implicating it in the pathogenesis of diseases such as Alzheimer's. Its interaction with p53-responsive elements also indicates its influence on tumor suppressor gene networks linking it to cancer biology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-SUPT4H antibody [EPR6100(2)] - BSA and Azide free (ab248339), expandable thumbnail](https://content.abcam.com/products/images/supt4h-antibody-epr61002-bsa-and-azide-free-ab248339--western-blot-img104627.jpg "Western blot - Anti-SUPT4H antibody [EPR6100(2)] - BSA and Azide free (ab248339)")
Western blot - Anti-SUPT4H antibody [EPR6100(2)] - BSA and Azide free (ab248339)
This data was developed using Anti-SUPT4H antibody [EPR6100(2)] ab129187, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SUPT4H antibody [EPR6100(2)] (Anti-SUPT4H antibody [EPR6100(2)] ab129187) at 1/1000 dilution
Lane 1: 293T cell lysates at 10 µg
Lane 2: MCF-7 cell lysates at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 13 kDa
![OI-RD Scanning - Anti-SUPT4H antibody [EPR6100(2)] - BSA and Azide free (ab248339), expandable thumbnail](https://content.abcam.com/products/images/supt4h-antibody-epr61002-bsa-and-azide-free-ab248339--oi-rd-scanning-img83719.jpg "OI-RD Scanning - Anti-SUPT4H antibody [EPR6100(2)] - BSA and Azide free (ab248339)")
OI-RD Scanning - Anti-SUPT4H antibody [EPR6100(2)] - BSA and Azide free (ab248339)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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