JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB248339

Anti-SUPT4H antibody [EPR6100(2)] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal SUPT4H antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SPT4H, SUPT4H, SUPT4H1, Transcription elongation factor SPT4, hSPT4, DRB sensitivity-inducing factor 14 kDa subunit, DRB sensitivity-inducing factor small subunit, DSIF p14, DSIF small subunit

2 Images
Western blot - Anti-SUPT4H antibody [EPR6100(2)] - BSA and Azide free (AB248339)
  • WB

Unknown

Western blot - Anti-SUPT4H antibody [EPR6100(2)] - BSA and Azide free (AB248339)

This data was developed using ab129187, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SUPT4H antibody [EPR6100(2)] (<a href='/en-us/products/primary-antibodies/supt4h-antibody-epr61002-ab129187'>ab129187</a>) at 1/1000 dilution

Lane 1:

293T cell lysates at 10 µg

Lane 2:

MCF-7 cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 13 kDa

false

OI-RD Scanning - Anti-SUPT4H antibody [EPR6100(2)] - BSA and Azide free (AB248339)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SUPT4H antibody [EPR6100(2)] - BSA and Azide free (AB248339)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6100(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

ab248339 is the carrier-free version of ab129187.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SUPT4H also known as Suppressor of Ty 4 Homolog or SPT4 Homolog functions as part of the transcription machinery. It forms a complex known as DSIF which involves SUPT4H and a partner protein called SPT5. SUPT4H modulates RNA polymerase II (RNAPII) by forming a complex with SPT5 and facilitating transcription elongation. The molecular mass of SUPT4H is approximately 15 kDa. Expression of SUPT4H occurs broadly across various tissues indicating its essential role in general transcriptional regulation.
Biological function summary

SUPT4H plays an important role in regulating gene expression through its involvement in the DSIF complex. The DSIF complex acts as a transcription factor that associates with paused polymerase influencing transcription elongation. SUPT4H's interaction with SPT5 is important for maintaining the DSIF complex stability and function. SUPT4H and its associated complex help ensure that transcription proceeds efficiently allowing proper gene expression and cellular function.

Pathways

The DSIF complex containing SUPT4H is integral to several critical gene regulatory networks. It is involved in transcriptional pausing and control within the immediate early gene response pathway interfacing with various transcription factors and pause-release factors. Moreover the interaction with NELF (Negative Elongation Factor) provides a checkpoint control in the regulation of RNA polymerase II pausing. These interactions synchronize transcription with RNA processing and output maintaining cellular homeostasis.

SUPT4H's role in transcription regulation suggests implications in cancer and neurodegenerative disorders. Altered expression and function can disrupt normal gene expression potentially leading to oncogenesis. In the context of neurodegenerative disorders SUPT4H's regulatory activity might affect neuronal gene expression implicating it in the pathogenesis of diseases such as Alzheimer's. Its interaction with p53-responsive elements also indicates its influence on tumor suppressor gene networks linking it to cancer biology.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
See full target information SUPT4H1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell proliferation 56:e13400 PubMed36642844

2023

Non-SMC condensin I complex subunit H participates in anti-programmed cell death-1 resistance of clear cell renal cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Chen,Weiqiang Ruan,Chunhao Guo,Ke Chen,Le Li,Jihua Tian,Zhiquan Hu,Dan Peng,Xing Zeng
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB133631

Anti-ESD antibody [EPR8447]

RabMAb|Recombinant|KO Validated

Western blot - Anti-ESD antibody [EPR8447] (AB133631)

  • 0
  • 0
Primary Antibodies

AB131237

Anti-TUG antibody [EPR8615]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUG antibody [EPR8615] (AB131237)

  • 1
  • 1
Primary Antibodies

AB248172

Anti-CPSF2/CPSF100 antibody [EPR7599] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-CPSF2/CPSF100 antibody [EPR7599] - BSA and Azide free (AB248172)

  • 0
  • 0
Primary Antibodies

AB129108

Anti-SF2 antibody [EPR8239]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SF2 antibody [EPR8239] (AB129108)

  • 2
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com