Rabbit Recombinant Monoclonal SUR1 antibody. Suitable for Flow Cyt (Intra), ICC/IF, WB and reacts with Human, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | Flow Cyt (Intra) | ICC/IF | IHC-Fr | IHC-P | WB | |
---|---|---|---|---|---|---|
Human | Not recommended | Tested | Tested | Not recommended | Not recommended | Expected |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Expected | Expected | Not recommended | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Subunit of the beta-cell ATP-sensitive potassium channel (KATP). Regulator of ATP-sensitive K(+) channels and insulin release.
HRINS, SUR, SUR1, SUR1, ABCC8, HRINS, SUR, ATP-binding cassette sub-family C member 8, Sulfonylurea receptor 1
Rabbit Recombinant Monoclonal SUR1 antibody. Suitable for Flow Cyt (Intra), ICC/IF, WB and reacts with Human, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR25761-44
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
The SUR1 protein also known as ABCC8 is an important subunit of ATP-sensitive potassium (K_ATP) channels. This protein has a molecular mass of approximately 158 kDa. SUR1 is expressed mainly in pancreatic beta cells although one can also find it in neurons and cardiac tissue. It plays an essential role in regulating the membrane potential and in turn controlling insulin secretion. SUR1 is a member of the ATP-binding cassette (ABC) transporter family which contributes to its ability to influence cellular responses to changes in metabolic states.
SUR1 binds with the Kir6.2 channel subunits to form functional K_ATP channel complexes. These channels respond to intracellular ATP and ADP levels providing a critical link between cellular energy metabolism and electric cell function. In pancreatic beta cells the opening and closing of these channels trigger the release of insulin in response to blood glucose levels. In the brain the channels modulate neuronal excitability and play a role in neuroprotection. The coupling with Kir6.2 emphasizes the role of the SUR1-Kir6.2 complex in many cellular processes.
The K_ATP channels containing SUR1 participate in the insulin secretion pathway and the cellular response to hypoxia. The insulin secretion pathway is important for glucose homeostasis where SUR1 interacts with proteins like Kir6.2 directly linking glucose metabolism to insulin release. In the cellular response to hypoxia SUR1 influences neuronal survival by modifying cell membrane potential regulating excitability and managing cellular energy usage in low oxygen conditions. These pathways demonstrate the importance of SUR1 in maintaining energy balance within cells and across organs.
SUR1 has a significant role in conditions such as congenital hyperinsulinism and neonatal diabetes. Mutations in the SUR1 gene (ABCC8) can lead to deregulation of insulin secretion causing the overproduction of insulin seen in congenital hyperinsulinism. Similarly other mutations might result in impaired insulin release contributing to neonatal diabetes. Additionally SUR1 interacts with Kir6.2 during these disorders and this interaction becomes a target for therapeutic strategies aimed at either activating or inhibiting K_ATP channels as needed to restore balanced insulin levels.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Kidney (PMID:17535866 ), HEK-293(PMID:23255597, 32332165 )
The expression molecular weight observed in lane 1 is consistent with what has been described in the literature (PMID: 21674179, 17657312)
Samples are non-boiled as boiling may cause protein aggregates.
Lane 1 of this blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
Exposure time:
Lane 1: 3 minutes
Lane 2, 3, 6, 7: 26 seconds
Lane 4, 5: 70 seconds
All lanes: Western blot - Anti-SUR1 antibody [EPR25761-44] (ab302611) at 1/1000 dilution
Lane 1: Human pancreas tissue lysate 20 µg at 20 µg
Lane 2: Human hypothalamus tissue lysate 20 µg at 20 µg
Lane 3: Human kidney tissue lysate 20 µg at 20 µg
Lane 4: SK-N-BE(2) (human neuroblastoma neuroblast), whole cell lysate 20 µg at 20 µg
Lane 5: HEK-293 (human embryonic kidney epithelial cell), whole cell lysate 20 µg at 20 µg
Lane 6: Rat hypothalamus tissue lysate 20 µg at 20 µg
Lane 7: Rat kidney tissue lysate 20 µg at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 150 kDa, 180 kDa
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293 (human embryonic kidney epithelial cell, Left) / SK-N-BE(2) (human neuroblastoma neuroblast, Right) cells labelling SUR1 with ab302611 at 1/5000 dilution (0.01ug) (Red) compared with a isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: HEK-293 (PMID: 23255597).
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-N-BE(2) (human neuroblastoma neuroblast) cells labelling SUR1 with ab302611 at 1/100 (5.37 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in SK-N-BE(2) cell lineNegative control: HEK-293 (PMID: 23255597? is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
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