Skip to main content

Rabbit Recombinant Monoclonal SUR1 antibody. Suitable for Flow Cyt (Intra), ICC/IF, WB and reacts with Human, Rat samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-SUR1 antibody [EPR25761-44] (AB302611), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SUR1 antibody [EPR25761-44] (AB302611), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SUR1 antibody [EPR25761-44] (AB302611), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-FrIHC-PWB
Human
Not recommended
Tested
Tested
Not recommended
Not recommended
Expected
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Expected
Expected
Not recommended
Not recommended
Tested

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/5000

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Rat

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Target data

Function

Subunit of the beta-cell ATP-sensitive potassium channel (KATP). Regulator of ATP-sensitive K(+) channels and insulin release.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal SUR1 antibody. Suitable for Flow Cyt (Intra), ICC/IF, WB and reacts with Human, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR25761-44

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The SUR1 protein also known as ABCC8 is an important subunit of ATP-sensitive potassium (K_ATP) channels. This protein has a molecular mass of approximately 158 kDa. SUR1 is expressed mainly in pancreatic beta cells although one can also find it in neurons and cardiac tissue. It plays an essential role in regulating the membrane potential and in turn controlling insulin secretion. SUR1 is a member of the ATP-binding cassette (ABC) transporter family which contributes to its ability to influence cellular responses to changes in metabolic states.

Biological function summary

SUR1 binds with the Kir6.2 channel subunits to form functional K_ATP channel complexes. These channels respond to intracellular ATP and ADP levels providing a critical link between cellular energy metabolism and electric cell function. In pancreatic beta cells the opening and closing of these channels trigger the release of insulin in response to blood glucose levels. In the brain the channels modulate neuronal excitability and play a role in neuroprotection. The coupling with Kir6.2 emphasizes the role of the SUR1-Kir6.2 complex in many cellular processes.

Pathways

The K_ATP channels containing SUR1 participate in the insulin secretion pathway and the cellular response to hypoxia. The insulin secretion pathway is important for glucose homeostasis where SUR1 interacts with proteins like Kir6.2 directly linking glucose metabolism to insulin release. In the cellular response to hypoxia SUR1 influences neuronal survival by modifying cell membrane potential regulating excitability and managing cellular energy usage in low oxygen conditions. These pathways demonstrate the importance of SUR1 in maintaining energy balance within cells and across organs.

Associated diseases and disorders

SUR1 has a significant role in conditions such as congenital hyperinsulinism and neonatal diabetes. Mutations in the SUR1 gene (ABCC8) can lead to deregulation of insulin secretion causing the overproduction of insulin seen in congenital hyperinsulinism. Similarly other mutations might result in impaired insulin release contributing to neonatal diabetes. Additionally SUR1 interacts with Kir6.2 during these disorders and this interaction becomes a target for therapeutic strategies aimed at either activating or inhibiting K_ATP channels as needed to restore balanced insulin levels.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-SUR1 antibody [EPR25761-44] (ab302611), expandable thumbnail

    Western blot - Anti-SUR1 antibody [EPR25761-44] (ab302611)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Kidney (PMID:17535866 ), HEK-293(PMID:23255597, 32332165 )

    The expression molecular weight observed in lane 1 is consistent with what has been described in the literature (PMID: 21674179, 17657312)

    Samples are non-boiled as boiling may cause protein aggregates.

    Lane 1 of this blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    Exposure time:

    Lane 1: 3 minutes

    Lane 2, 3, 6, 7: 26 seconds

    Lane 4, 5: 70 seconds

    All lanes: Western blot - Anti-SUR1 antibody [EPR25761-44] (ab302611) at 1/1000 dilution

    Lane 1: Human pancreas tissue lysate 20 µg at 20 µg

    Lane 2: Human hypothalamus tissue lysate 20 µg at 20 µg

    Lane 3: Human kidney tissue lysate 20 µg at 20 µg

    Lane 4: SK-N-BE(2) (human neuroblastoma neuroblast), whole cell lysate 20 µg at 20 µg

    Lane 5: HEK-293 (human embryonic kidney epithelial cell), whole cell lysate 20 µg at 20 µg

    Lane 6: Rat hypothalamus tissue lysate 20 µg at 20 µg

    Lane 7: Rat kidney tissue lysate 20 µg at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 150 kDa, 180 kDa

  • Flow Cytometry (Intracellular) - Anti-SUR1 antibody [EPR25761-44] (ab302611), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SUR1 antibody [EPR25761-44] (ab302611)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293 (human embryonic kidney epithelial cell, Left) / SK-N-BE(2) (human neuroblastoma neuroblast, Right) cells labelling SUR1 with ab302611 at 1/5000 dilution (0.01ug) (Red) compared with a isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: HEK-293 (PMID: 23255597).

  • Immunocytochemistry/ Immunofluorescence - Anti-SUR1 antibody [EPR25761-44] (ab302611), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SUR1 antibody [EPR25761-44] (ab302611)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-N-BE(2) (human neuroblastoma neuroblast) cells labelling SUR1 with ab302611 at 1/100 (5.37 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in SK-N-BE(2) cell lineNegative control: HEK-293 (PMID: 23255597? is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com