Skip to main content

Mouse Monoclonal 6X His tag® antibody - conjugated to SureLight® APC. Suitable for Flow Cyt and reacts with Tag samples. Cited in 9 publications.

Be the first to review this product! Submit a review

Images

Flow Cytometry - SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (AB72579), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Conjugation

SureLight® APC

Storage buffer

pH: 7.4
Preservative: 0.013% Sodium azide
Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 0.87% Sodium chloride, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Tag
Expected

Expected
Expected

Species

Tag

Dilution info

-

Notes

ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

6 products for Alternative Product

8 products for Alternative Version

Alternative names

Recommended products

Mouse Monoclonal 6X His tag® antibody - conjugated to SureLight® APC. Suitable for Flow Cyt and reacts with Tag samples. Cited in 9 publications.

Key facts

Isotype

IgG1

Conjugation

SureLight® APC

Form

Liquid

Clonality

Monoclonal

Clone number

AD1.1.10

Purification technique

Size-exclusion chromatography

Concentration
Loading...
Purification notes

Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

SureLight® Allophycocyanin. Excitation max. 652 nm, Emission max. 657 nm. F/P 1:1.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

Associated diseases and disorders

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab72579), expandable thumbnail

    Flow Cytometry - SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab72579)

    Flow cytometric analysis of transfected HEK293 cells labelling 6X His tag® with ab72579 at 1 µg/mL.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com