AB72579

SureLight® APC Anti-6X His tag® antibody [AD1.1.10]

Name: SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab72579) | Abcam
Brand: Abcam Limited
SKU: AB72579
Price: 330 USD
Availability: InStock

Be the first to review this product! Submit a review

(12 Publications)

Anti-6X His tag® antibody [AD1.1.10] - SureLight® APC conjugated (ab72579) is a mouse monoclonal antibody detecting 6X His tag® in Flow Cytometry.

- Trusted since 2008

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

1 Images

Flow Cytometry - SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (AB72579)

Flow Cyt

Supplier Data

Flow Cytometry - SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (AB72579)

Flow cytometric analysis of transfected HEK293 cells labelling 6X His tag® with ab72579 at 1 µg/mL.

673 DyLight® 650

DyLight® 650 Anti-6X His tag® antibody [AD1.1.10]
Unconjugated

Anti-6X His tag® antibody [AD1.1.10]
Alkaline Phosphatase

Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10]
518 DyLight® 488

DyLight® 488 Anti-6X His tag® antibody [AD1.1.10]
576 DyLight® 550

DyLight® 550 Anti-6X His tag® antibody [AD1.1.10]
HRP

HRP Anti-6X His tag® antibody [AD1.1.10]
578 PE

PE Anti-6X His tag® antibody [AD1.1.10]
675 PerCP

PerCP Anti-6X His tag® antibody [AD1.1.10]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AD1.1.10

Isotype

IgG1

Conjugation

SureLight® APC

Excitation/Emission

Ex: 652nm, Em: 657nm

Carrier free

Applications

Flow Cyt

applications

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/apc-mouse-fab2-igg1-kappa-monoclonal-15h6-isotype-control-ab37391'>ab37391</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

style

left-column

Product details

What is this antibody validated in?
SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab72579) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt). Tagged protein detection
6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Related products
Antibody clone AD1.1.10 is also available pre-conjugated to a variety of labels for your convenience – Anti-6X His tag® SureLight® APC [AD1.1.10] (ab72579).

Other related products
We have a range of other formats of antibody clone [AD1.1.10] also available for your convenience: ab15149, PE - ab72467, SureLight® APC - ab72579, Alkaline Phosphatase - ab81652, PerCP - ab117496, Carrier free - ab117504, DyLight® 550 - ab117507, DyLight® 488 - ab117512, HRP - ab178563, Carrier free - ab307236

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.

Storage buffer

pH: 7.4 Preservative: 0.013% Sodium azide Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 0.87% Sodium chloride, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information 6X His tag®

style

left-column

Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Protein science : a publication of the Protein Society 34:e70072 PubMed39981925

2025

Taming interleukin-12: Engineering of bispecific antibody-based IL-12 mimetics with biased agonism capacities.

Applications

Unspecified application

Species

Unspecified reactive species

Britta Lipinski,Laura Unmuth,Paul Arras,Ron Endruszeit,Stefan Becker,Jonathan Mathias Rödel,Jürgen Scheller,Silke Pudewell,Doreen M Floss,Simon Krah,Julia Harwardt,Achim Doerner,Laura Helming,Chunxiao Xu,Andreas Menrad,Andreas Evers,Harald Kolmar,Desislava Elter,Lukas Pekar,Stefan Zielonka

mAbs 16:2406548 PubMed39304998

2024

Discovery of potent allosteric antibodies inhibiting EGFR.

Applications

Unspecified application

Species

Unspecified reactive species

Léxane Fournier,Lukas Pekar,Birgitta Leuthner,Harald Kolmar,Lars Toleikis,Stefan Becker

Nature communications 15:812 PubMed38280880

2024

The cryo-EM structure of homotetrameric attachment glycoprotein from langya henipavirus.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Guo,Songyue Wu,Wenting Li,Haonan Yang,Tianhao Shi,Bin Ju,Zheng Zhang,Renhong Yan

Journal of medical virology 95:e28642 PubMed36890630

2023

Pre-existing immunity to SARS-CoV-2 associates with strong T cell responses induced by inactivated COVID-19 vaccines.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Yi,Yuhao Wang,Quanrun Li,Xiaoyi Li,Panli Zhang,Xin Fu,Shuqin Gu,Daqian Zhang,Xiaoyi Liu,Haonan Lou,Yuemei Wu,Libo Tang,Jinlin Hou,Yongyin Li

iScience 25:104431 PubMed35607524

2022

Structural and functional analysis of an inter-Spike bivalent neutralizing antibody against SARS-CoV-2 variants.

Applications

Unspecified application

Species

Unspecified reactive species

Yaning Li,Qing Fan,Bing Zhou,Yaping Shen,Yuanyuan Zhang,Lin Cheng,Furong Qi,Shuo Song,Yingying Guo,Renhong Yan,Bin Ju,Zheng Zhang

Glycoconjugate journal 39:315-325 PubMed34699015

2021

Structural characteristics of Heparan sulfate required for the binding with the virus processing Enzyme Furin.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxin Zeng,Yuan Meng,Shi-Yi Chen,Gaofeng Zhao,Lianchun Wang,En-Xin Zhang,Hong Qiu

Cell reports. Medicine 2:100420 PubMed34604818

2021

Intradermal-delivered DNA vaccine induces durable immunity mediating a reduction in viral load in a rhesus macaque SARS-CoV-2 challenge model.

Applications

Unspecified application

Species

Unspecified reactive species

Ami Patel,Jewell N Walters,Emma L Reuschel,Katherine Schultheis,Elizabeth Parzych,Ebony N Gary,Igor Maricic,Mansi Purwar,Zeena Eblimit,Susanne N Walker,Diana Guimet,Pratik Bhojnagarwala,Opeyemi S Adeniji,Arthur Doan,Ziyang Xu,Dustin Elwood,Sophia M Reeder,Laurent Pessaint,Kevin Y Kim,Anthony Cook,Neethu Chokkalingam,Brad Finneyfrock,Edgar Tello-Ruiz,Alan Dodson,Jihae Choi,Alison Generotti,John Harrison,Nicholas J Tursi,Viviane M Andrade,Yaya Dia,Faraz I Zaidi,Hanne Andersen,Mohamed Abdel-Mohsen,Mark G Lewis,Kar Muthumani,J Joseph Kim,Daniel W Kulp,Laurent M Humeau,Stephanie J Ramos,Trevor R F Smith,David B Weiner,Kate E Broderick

Cell reports 36:109525 PubMed34380042

2021

Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Peter Szodoray,Tor Kristian Andersen,Julia Heinzelbecker,John F Imbery,Peter C Huszthy,Stephanie M Stanford,Bjarne Bogen,Ole B Landsverk,Nunzio Bottini,Anders Tveita,Ludvig A Munthe,Britt Nakken

Nature 584:115-119 PubMed32454513

2020

Human neutralizing antibodies elicited by SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Ju,Qi Zhang,Jiwan Ge,Ruoke Wang,Jing Sun,Xiangyang Ge,Jiazhen Yu,Sisi Shan,Bing Zhou,Shuo Song,Xian Tang,Jinfang Yu,Jun Lan,Jing Yuan,Haiyan Wang,Juanjuan Zhao,Shuye Zhang,Youchun Wang,Xuanling Shi,Lei Liu,Jincun Zhao,Xinquan Wang,Zheng Zhang,Linqi Zhang

eLife 9: PubMed32314731

2020

Landscape mapping of shared antigenic epitopes and their cognate TCRs of tumor-infiltrating T lymphocytes in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kenji Murata,Munehide Nakatsugawa,Muhammed A Rahman,Linh T Nguyen,Douglas G Millar,David T Mulder,Kenji Sugata,Hiroshi Saijo,Yukiko Matsunaga,Yuki Kagoya,Tingxi Guo,Mark Anczurowski,Chung-Hsi Wang,Brian D Burt,Dalam Ly,Kayoko Saso,Alexandra Easson,David P Goldstein,Michael Reedijk,Danny Ghazarian,Trevor J Pugh,Marcus O Butler,Tak W Mak,Pamela S Ohashi,Naoto Hirano

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

SureLight® APC Anti-6X His tag® antibody [AD1.1.10]

Flow Cytometry - SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (AB72579)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise