Anti-DDDDK tag (Binds to FLAG® tag sequence) [M2] SureLight® APC conjugated antibody (ab72569) mouse monoclonal antibody that is used to detect DDDDK tag (Binds to FLAG® tag sequence) in Flow Cytometry (Intra), Flow Cytometry.
- Trusted since 2009
Images
![Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72569), expandable thumbnail](https://content.abcam.com/products/images/surelight-apc-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab72569--flow-cytometry-intracellular-img165225.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72569)")
Publications
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- eLife 11:2022Glycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJazlyn P Borges,Ragnhild S R Sætra,Allen Volchuk,Marit Bugge,Pascal Devant,Bjørnar Sporsheim,Bridget R Kilburn,Charles L Evavold,Jonathan C Kagan,Neil M Goldenberg,Trude Helen Flo,Benjamin Ethan SteinbergPubMed 36468682
- Synthetic biology (Oxford, England) 5:ysaa0172020Elucidation and refinement of synthetic receptor mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHailey I Edelstein et. al.PubMed 33392392
- The Journal of biological chemistry 293:17505-175222018Complex formation of sphingomyelin synthase 1 with glucosylceramide synthase increases sphingomyelin and decreases glucosylceramide levels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasuhiro Hayashi et. al.PubMed 30242129
- mAbs 10:416-4302018A novel bicistronic gene design couples stable cell line selection with a fucose switch in a designer CHO host to produce native and afucosylated glycoform antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGargi Roy et. al.PubMed 29400603
- The Journal of biological chemistry 292:1122-11412016Carboxyl-terminal Tail-mediated Homodimerizations of Sphingomyelin Synthases Are Responsible for Efficient Export from the Endoplasmic Reticulum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasuhiro Hayashi,Yoko Nemoto-Sasaki,Naoki Matsumoto,Takashi Tanikawa,Saori Oka,Yusuke Tanaka,Seisuke Arai,Ikuo Wada,Takayuki Sugiura,Atsushi YamashitaPubMed 27927984
- Journal of virology 89:11643-532015Computational and Functional Analysis of the Virus-Receptor Interface Reveals Host Range Trade-Offs in New World Arenaviruses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesScott A Kerr et. al.PubMed 26355089
- PLoS biology 11:e10015712013Dual host-virus arms races shape an essential housekeeping protein.Applications:Flow CytReactive species:Unspecified reactive speciesAnn Demogines et. al.PubMed 23723737
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- SureLight® APC
- Storage buffer
pH: 7.4
Preservative: 0.013% Sodium azide
Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 0.87% Sodium chloride, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| Flow Cyt (Intra) | FRET | |
|---|---|---|
| Tag | Not recommended | Expected |
| Transfected cell lysate | Expected | Not recommended |
Flow Cyt (Intra)
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
FRET
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
8 products for Alternative Version
Alternative names
DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk
Recommended products
Anti-DDDDK tag (Binds to FLAG® tag sequence) [M2] SureLight® APC conjugated antibody (ab72569) mouse monoclonal antibody that is used to detect DDDDK tag (Binds to FLAG® tag sequence) in Flow Cytometry (Intra), Flow Cytometry.
- Trusted since 2009
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- SureLight® APC
- Storage buffer
pH: 7.4
Preservative: 0.013% Sodium azide
Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 0.87% Sodium chloride, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- M2
- Purification technique
- Size-exclusion chromatography
- Concentration
- Purification notes
Purified using size exclusion chromatography as well as some proprietary methods for purifying the final materials.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Store in the dark
Notes
SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt).
Tagged protein detection
DDDDK tag (Binds to FLAG® tag sequence) antibodies are used to visualize proteins labelled with this tag in a variety of applications.
Related products
Antibody clone M2 is also available pre-conjugated to a variety of labels for your convenience – Anti-DDDDK tag (Binds to FLAG® tag sequence) SureLight® APC [M2] (ab72569).
Other related products
We have a range of other formats of antibody clone [M2] also available for your convenience:
Alkaline Phosphatase - Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] ab81655, DyLight® 650 - DyLight® 650 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] ab117492, DyLight® 550 - DyLight® 550 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] ab117495, Carrier free - DyLight® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] ab117505, PerCP - PerCP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] ab117514, Europium - Europium Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] ab187914
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569), expandable thumbnail](https://content.abcam.com/products/images/surelight-apc-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab72569--flow-cytometry-intracellular-img165225.jpg "Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569)")
Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569)
Flow Cytometry analysis of transfected HEK293 cells. Fixed and permeabilized cells stained with 1 μg/ml of ab72569
- Shaded histogram – HEK293 cells, expressing DYKDDDDK-tagged protein
- Open histogram – mock transfected control HEK293 cellsNo exactly the same info
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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