Mouse Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody - conjugated to SureLight® APC. Suitable for Flow Cyt (Intra), FRET and reacts with Transfected cell lysate, Tag samples. Cited in 7 publications.
Images
![Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72569), expandable thumbnail](https://content.abcam.com/products/images/surelight-apc-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab72569--flow-cytometry-intracellular-img165225.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72569)")
Publications
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- eLife 11:2022Glycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJazlyn P Borges,Ragnhild S R Sætra,Allen Volchuk,Marit Bugge,Pascal Devant,Bjørnar Sporsheim,Bridget R Kilburn,Charles L Evavold,Jonathan C Kagan,Neil M Goldenberg,Trude Helen Flo,Benjamin Ethan SteinbergPubMed 36468682
- Synthetic biology (Oxford, England) 5:ysaa0172020Elucidation and refinement of synthetic receptor mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHailey I Edelstein et. al.PubMed 33392392
- The Journal of biological chemistry 293:17505-175222018Complex formation of sphingomyelin synthase 1 with glucosylceramide synthase increases sphingomyelin and decreases glucosylceramide levels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasuhiro Hayashi et. al.PubMed 30242129
- mAbs 10:416-4302018A novel bicistronic gene design couples stable cell line selection with a fucose switch in a designer CHO host to produce native and afucosylated glycoform antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGargi Roy et. al.PubMed 29400603
- The Journal of biological chemistry 292:1122-11412016Carboxyl-terminal Tail-mediated Homodimerizations of Sphingomyelin Synthases Are Responsible for Efficient Export from the Endoplasmic Reticulum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasuhiro Hayashi,Yoko Nemoto-Sasaki,Naoki Matsumoto,Takashi Tanikawa,Saori Oka,Yusuke Tanaka,Seisuke Arai,Ikuo Wada,Takayuki Sugiura,Atsushi YamashitaPubMed 27927984
- Journal of virology 89:11643-532015Computational and Functional Analysis of the Virus-Receptor Interface Reveals Host Range Trade-Offs in New World Arenaviruses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesScott A Kerr et. al.PubMed 26355089
- PLoS biology 11:e10015712013Dual host-virus arms races shape an essential housekeeping protein.Applications:Flow CytReactive species:Unspecified reactive speciesAnn Demogines et. al.PubMed 23723737
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
SureLight® APC
- Storage buffer
pH: 7.4
Preservative: 0.013% Sodium azide
Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 0.87% Sodium chloride, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
Reactivity data
Select an application| Flow Cyt (Intra) | FRET | |
|---|---|---|
| Tag | Not recommended | Expected |
| Transfected cell lysate | Expected | Not recommended |
Flow Cyt (Intra)
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
FRET
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
8 products for Alternative Version
Alternative names
DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk
Recommended products
Mouse Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody - conjugated to SureLight® APC. Suitable for Flow Cyt (Intra), FRET and reacts with Transfected cell lysate, Tag samples. Cited in 7 publications.
Alternative names
DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
SureLight® APC
- Storage buffer
pH: 7.4
Preservative: 0.013% Sodium azide
Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 0.87% Sodium chloride, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
- Clone number
M2
- Purification technique
Size-exclusion chromatography
- Concentration
- Purification notes
Purified using size exclusion chromatography as well as some proprietary methods for purifying the final materials.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Store in the dark
Notes
This antibody has been optimized for High throughput screening (HTS) and fluorescence resonance energy transfer (FRET) assays.
Excitation maximum:652 nm Emission maximum:657.5 nm F:P 1:1.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569), expandable thumbnail](https://content.abcam.com/products/images/surelight-apc-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab72569--flow-cytometry-intracellular-img165225.jpg "Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569)")
Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569)
Flow Cytometry analysis of transfected HEK293 cells. Fixed and permeabilized cells stained with 1 μg/ml of ab72569
- Shaded histogram – HEK293 cells, expressing DYKDDDDK-tagged protein
- Open histogram – mock transfected control HEK293 cellsNo exactly the same info
Downloads
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