AB72569

SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]

Name: SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569) | Abcam
Brand: Abcam Limited
SKU: AB72569
Price: 410 USD
Availability: InStock

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(13 Publications)

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] - SureLight® APC conjugated (ab72569) is a mouse monoclonal antibody detecting DDDDK tag (Binds to FLAG® tag sequence) in Flow Cytometry (Intra).

- Trusted since 2009

1 Images

Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72569)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72569)

Flow Cytometry analysis of transfected HEK293 cells. Fixed and permeabilized cells stained with 1 μg/ml of ab72569

Shaded histogram – HEK293 cells, expressing DYKDDDDK-tagged protein
Open histogram – mock transfected control HEK293 cellsNo exactly the same info

518 DyLight® 488

DyLight® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
Alkaline Phosphatase

Alkaline Phosphatase Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
576 DyLight® 550

DyLight® 550 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
673 DyLight® 650

DyLight® 650 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
615 Europium

Europium Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
HRP

HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
578 PE

PE Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]
675 PerCP

PerCP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG1

Conjugation

SureLight® APC

Excitation/Emission

Ex: 652nm, Em: 657nm

Carrier free

Applications

Flow Cyt (Intra), FRET

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "FRET" : {"fullname" : "FRET", "shortname":"FRET"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "FRET-species-checked": "guaranteed", "FRET-species-dilution-info": "", "FRET-species-notes": "<p></p>" }, "Transfected cell lysate": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "FRET-species-checked": "notRecommended", "FRET-species-dilution-info": "", "FRET-species-notes": "" } } }

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Product details

What is this antibody validated in?
SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab72569) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt). Tagged protein detection
DDDDK tag (Binds to FLAG® tag sequence) antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Related products
Antibody clone M2 is also available pre-conjugated to a variety of labels for your convenience – Anti-DDDDK tag (Binds to FLAG® tag sequence) SureLight® APC [M2] (ab72569).

Other related products
We have a range of other formats of antibody clone [M2] also available for your convenience: Alkaline Phosphatase - ab81655, DyLight® 650 - ab117492, DyLight® 550 - ab117495, Carrier free - ab117505, PerCP - ab117514, Europium - ab187914

FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Purified using size exclusion chromatography as well as some proprietary methods for purifying the final materials.

Storage buffer

pH: 7.4 Preservative: 0.013% Sodium azide Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 0.87% Sodium chloride, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information DDDDK tag (Binds to FLAG® tag sequence)

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8403 PubMed40998787

2025

Structural basis for ligand promiscuity and high signaling activity of Kaposi's Sarcoma-associated Herpesvirus-encoded GPCR.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Bae Park,Bibekananda Sahoo,Amita Rani Sahoo,Dokyun Kim,Hogyu David Seo,James Bowman,Mi-Jeong Kwak,Sophia Suh,Matthias Buck,Xinghong Dai,Jae U Jung

Nature chemical biology 21:1719-1730 PubMed40846994

2025

Conversion of natural cytokine receptors into orthogonal synthetic biosensors.

Applications

Unspecified application

Species

Unspecified reactive species

Hailey I Edelstein,Amparo Cosio,Max L Ezekiel,William K Corcoran,Aaron H Morris,Joshua N Leonard

Cell 187:5554-5571.e19 PubMed39197450

2024

Mosaic sarbecovirus nanoparticles elicit cross-reactive responses in pre-vaccinated animals.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander A Cohen,Jennifer R Keeffe,Ariën Schiepers,Sandra E Dross,Allison J Greaney,Annie V Rorick,Han Gao,Priyanthi N P Gnanapragasam,Chengcheng Fan,Anthony P West,Arlene I Ramsingh,Jesse H Erasmus,Janice D Pata,Hiromi Muramatsu,Norbert Pardi,Paulo J C Lin,Scott Baxter,Rita Cruz,Martina Quintanar-Audelo,Ellis Robb,Cristina Serrano-Amatriain,Leonardo Magneschi,Ian G Fotheringham,Deborah H Fuller,Gabriel D Victora,Pamela J Bjorkman

Nature biomedical engineering 8:397-414 PubMed38012307

2023

Genetically encoding multiple functionalities into extracellular vesicles for the targeted delivery of biologics to T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Devin M Stranford,Lacy M Simons,Katherine E Berman,Luyi Cheng,Beth N DiBiase,Michelle E Hung,Julius B Lucks,Judd F Hultquist,Joshua N Leonard

eLife 11: PubMed36468682

2022

Glycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Jazlyn P Borges,Ragnhild S R Sætra,Allen Volchuk,Marit Bugge,Pascal Devant,Bjørnar Sporsheim,Bridget R Kilburn,Charles L Evavold,Jonathan C Kagan,Neil M Goldenberg,Trude Helen Flo,Benjamin Ethan Steinberg

Synthetic biology (Oxford, England) 5:ysaa017 PubMed33392392

2020

Elucidation and refinement of synthetic receptor mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Hailey I Edelstein,Patrick S Donahue,Joseph J Muldoon,Anthony K Kang,Taylor B Dolberg,Lauren M Battaglia,Everett R Allchin,Mihe Hong,Joshua N Leonard

mAbs 12:1684749 PubMed31775561

2019

Development of a high yielding expression platform for the introduction of non-natural amino acids in protein sequences.

Applications

Unspecified application

Species

Unspecified reactive species

Gargi Roy,Jason Reier,Andrew Garcia,Tom Martin,Megan Rice,Jihong Wang,Meagan Prophet,Ronald Christie,William Dall'Acqua,Sanjeev Ahuja,Michael A Bowen,Marcello Marelli

The Journal of biological chemistry 293:17505-17522 PubMed30242129

2018

Complex formation of sphingomyelin synthase 1 with glucosylceramide synthase increases sphingomyelin and decreases glucosylceramide levels.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuhiro Hayashi,Yoko Nemoto-Sasaki,Naoki Matsumoto,Kotaro Hama,Takashi Tanikawa,Saori Oka,Tadaaki Saeki,Tatsuya Kumasaka,Takanori Koizumi,Seisuke Arai,Ikuo Wada,Kazuaki Yokoyama,Takayuki Sugiura,Atsushi Yamashita

mAbs 10:416-430 PubMed29400603

2018

A novel bicistronic gene design couples stable cell line selection with a fucose switch in a designer CHO host to produce native and afucosylated glycoform antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Gargi Roy,Tom Martin,Arnita Barnes,Jihong Wang,Rod Brian Jimenez,Megan Rice,Lina Li,Hui Feng,Shu Zhang,Raghothama Chaerkady,Herren Wu,Marcello Marelli,Diane Hatton,Jie Zhu,Michael A Bowen

Nature chemical biology 13:202-209 PubMed27941759

2016

Rewiring human cellular input-output using modular extracellular sensors.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly A Schwarz,Nichole M Daringer,Taylor B Dolberg,Joshua N Leonard

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]

Flow Cytometry (Intracellular) - SureLight® APC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB72569)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (13)

Product promise