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AB72565

SureLight® APC Anti-T7 tag® antibody

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(1 Publication)

Rabbit Polyclonal T7 tag® antibody - conjugated to SureLight® APC. Suitable for Flow Cyt and reacts with Tag samples. Cited in 1 publication.
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Flow Cytometry - SureLight® APC Anti-T7 tag® antibody (AB72565)
  • Flow Cyt

Supplier Data

Flow Cytometry - SureLight® APC Anti-T7 tag® antibody (AB72565)

Flow cytometry analysis of a recombinant (N-terminus T7-tag) transmembrane cell surface receptor in CHO cells using ab72565 at a 1/20 dilution (yellow). Black - Mouse IgG SureLight® Allophycocyanin isotype control.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

SureLight® APC

Excitation/Emission

Ex: 652nm, Em: 657nm

Carrier free

No

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Excitation maximum:650 nm Emission maximum:660 nm
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Size exclusion chromatography as well as some proprietary methods for purifying the final materials werer used.
Storage buffer
pH: 7.4 Preservative: 0.013% Sodium azide Constituents: 1.71% Sucrose, 1.64% Sodium phosphate, 0.87% Sodium chloride, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The T7 tag is a 11-amino acid peptide derived from the T7 major capsid protein commonly used as an epitope tag for protein purification and detection. This tag has a size of approximately 1371 Da (Dalton) and is highly recognizable due to its specific sequence and affinity for antibodies. Researchers frequently express the T7 tag in various systems including prokaryotic and eukaryotic cells to facilitate the purification process and Western blot analyses. It is also sometimes referred to by the notation "-7 = t/7" due to its tag characteristics.
Biological function summary

The T7 tag does not inherently perform functional roles in cellular processes; instead it serves as a tool to analyze target proteins. Researchers attach the T7 tag to proteins of interest allowing the tagged complex to be isolated using affinity tags such as biotin or alkaline phosphatase (-ALP) conjugates. The T7 tag's high affinity for its specific antibodies makes it an efficient choice for detection using Western blot methods including HRP Western blot and biotin size kDa assessment.

Pathways

The T7 tag does not directly interact with any specific biological pathways since its function as a tag is mainly technical. However it facilitates the study of pathways by enabling the isolation and analysis of proteins involved in key signaling cascades like the MAPK and PI3K/AKT pathways. Researchers use the T7 tag to explore protein-protein interactions and post-translational modifications within these pathways studying associated proteins such as kinases and regulatory enzymes.

The T7 tag itself is not a contributor to pathologies but assists researchers in understanding disease mechanisms. This tag plays a critical role in studying proteins implicated in specific conditions like cancer and neurodegenerative diseases. For instance using the T7 tag scientists can study proteins altered in these diseases often in conjunction with proteins such as p53 in cancer or alpha-synuclein in neurodegenerative disorders. This research can uncover therapeutic targets and deepen understanding of disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information T7 tag®

Additional targets

T7 tag®
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 287:10414-23 PubMed22318735

2012

Mapping human protease-activated receptor 4 (PAR4) homodimer interface to transmembrane helix 4.

Applications

Flow Cyt

Species

Unspecified reactive species

María de la Fuente,Daniel N Noble,Sheetal Verma,Marvin T Nieman
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