JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB72560

SureLight® APC Anti-V5 tag antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal V5 tag antibody - conjugated to SureLight® APC. Suitable for Flow Cyt, sELISA and reacts with Tag samples. Cited in 1 publication.

View Alternative Names

GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, V5 epitope tag

2 Images
Flow Cytometry - SureLight® APC Anti-V5 tag antibody (AB72560)
  • Flow Cyt

Supplier Data

Flow Cytometry - SureLight® APC Anti-V5 tag antibody (AB72560)

Flow cytometry analysis of a recombinant (N-terminus V5-tag) transmembrane cell surface receptor in CHO cells using ab72560 at a 1/20 dilution (red). Black - Mouse IgG SureLight® Allophycocyanin isotype control.

Sandwich ELISA - SureLight® APC Anti-V5 tag antibody (AB72560)
  • sELISA

Unknown

Sandwich ELISA - SureLight® APC Anti-V5 tag antibody (AB72560)

Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated V5-tagged peptide and read after a 2 hour incubation at room temperature with ab72560.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

SureLight® APC

Excitation/Emission

Ex: 652nm, Em: 657nm

Carrier free

No

Applications

Flow Cyt, sELISA

applications

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

Excitation maximum: 650 nm Emission maximum: 660 nm
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.
Storage buffer
pH: 7.4 Preservative: 0.013% Sodium azide Constituents: 1.64% Sodium phosphate, 0.87% Sodium chloride, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The V5 tag is an epitope tag derived from the P and V fusion proteins of the paramyxovirus. It typically measures around 14 amino acids in length and is often used in molecular biology for tagging and protein expression studies. In terms of mechanical function the V5 tag facilitates the detection characterization and purification of recombinant proteins. Scientists commonly use it in various hosts such as bacteria yeast and mammalian cells due to its convenient integration into larger protein complexes. Unlike native proteins it serves as a non-disruptive label for identifying proteins without affecting their functionality.
Biological function summary

Researchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.

Pathways

The tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.

Scientists harness the V5 tag to investigate the expression and interactions of proteins associated with certain conditions such as cancer and neurodegenerative diseases. Through its integration scientists aim to uncover alterations in protein interactions or localizations that contribute to disease progression. For example they may tag proteins linked to cancer pathways observing how disruptions in proteins related to anti-V5 targets could provide insights into tumor biology or pathogenesis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information V5 tag
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 36:268-287.e10 PubMed31447347

2019

GAPDH Overexpression in the T Cell Lineage Promotes Angioimmunoblastic T Cell Lymphoma through an NF-κB-Dependent Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Mondragón,Rana Mhaidly,Gian Marco De Donatis,Marie Tosolini,Pascal Dao,Anthony R Martin,Caroline Pons,Johanna Chiche,Marie Jacquin,Véronique Imbert,Emma Proïcs,Laurent Boyer,Anne Doye,Frédéric Luciano,Jaap G Neels,Frédéric Coutant,Nicole Fabien,Laura Sormani,Camila Rubio-Patiño,Jozef P Bossowski,Florian Muller,Sandrine Marchetti,Elodie Villa,Jean-François Peyron,Philippe Gaulard,François Lemonnier,Vahid Asnafi,Laurent Genestier,Rachid Benhida,Jean-Jacques Fournié,Thierry Passeron,Jean-Ehrland Ricci,Els Verhoeyen
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB206566

Anti-V5 tag antibody [SV5-P-K]

Recombinant

Indirect ELISA - Anti-V5 tag antibody [SV5-P-K] (AB206566)

  • 5
  • 1
Primary Antibodies

AB206571

Anti-V5 tag antibody [SV5-P-K]

Recombinant

ELISA - Anti-V5 tag antibody [SV5-P-K] (AB206571)

  • 0
  • 0
Primary Antibodies

AB206559

Anti-V5 tag antibody [SV5-P-K]

Recombinant

  • 0
  • 0
Primary Antibodies

AB15828

Anti-V5 tag antibody

Lab Essentials

Western blot - Anti-V5 tag antibody (AB15828)

  • 4
  • 1
Primary Antibodies

AB182008

Anti-V5 tag antibody [EPR12989]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-V5 tag antibody [EPR12989] (AB182008)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB202008

Alexa Fluor® 488 Anti-Myc tag antibody [9E10]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (AB202008)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB305269

Alexa Fluor® 488 Anti-HA tag antibody [EPR22819-101]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-HA tag antibody [EPR22819-101] (AB305269)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com