Anti-Surf1 antibody [21H2BG4]

• Flow Cyt

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Flow Cytometry - Anti-Surf1 antibody [21H2BG4] (AB110256)

Overlay histogram showing HepG2 cells stained with ab110256 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110256, 0.1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• WB

Lab

Western blot - Anti-Surf1 antibody [21H2BG4] (AB110256)

Complex IV assembly factor SURF1 identification. This mAb can be used to verify the presence of Complex IV assembly factor SURF1. Blotted alongside are mAbs against the ATP synthase subunit F1α subunit, an abundant mitochondrial protein acting as a control for gel loading. To indicate the assembly state of the cytochrome c oxidase, mAbs against the nuclear cytochrome c oxidase subunit COXVα and the mitochondrially encoded COXII subunit are also blotted in this Figure.

All lanes:

Western blot - Anti-Surf1 antibody [21H2BG4] (ab110256)

Predicted band size: 33 kDa

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