Anti-Surfactant Protein A/PSAP antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody (AB115791)

Surfactant Protein A/PSAP was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab115791 overnight at 4°C. Biotinylated goat antirabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody (AB115791)

Surfactant Protein A/PSAP was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab115791 overnight at 4°C. Biotinylated goat antirabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

• WB

Supplier Data

Western blot - Anti-Surfactant Protein A/PSAP antibody (AB115791)

The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.

All lanes:

Western blot - Anti-Surfactant Protein A/PSAP antibody (ab115791) at 0.5 µg/mL

Lane 1:

Rat lung tissue lysates with 5% non-fat milk/TBS for 1.5 hour at RT. at 30 µg

Lane 2:

Mouse lung tissue lysates with 5% non-fat milk/TBS for 1.5 hour at RT. at 30 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP at 1/5000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa,35 kDa

false

Exposure time: 3hr