Anti-Surfactant Protein A/PSAP antibody [EPR29097-290]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (AB322404)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Surfactant Protein A/PSAP with ab322404 at 1/5000 (0.092 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse spleen.
The section was incubated with ab322404 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (AB322404)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Surfactant Protein A/PSAP with ab322404 at 1/5000 (0.092 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in rat spleen.
The section was incubated with ab322404 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (AB322404)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lung tissue staining ATP-binding cassette sub-family A member 3 with ab324911 at a 1 : 2000 (0.253 ug/ml) dilution, ab322404 anti-PSAP used at 1 : 5000 (0.092 ug/ml) dilution and ab305303 anti-Aquaporin-5 used at a 1 : 2000 (0.265 ug/ml) dilution.

Panel A : merged staining of anti-ATP-binding cassette sub-family A member 3 (green; Opal™690), anti-PSAP (magenta; Opal™520) and anti-Aquaporin-5 (yellow; Opal™570) on mouse lung.
Panel B : anti-ATP-binding cassette sub-family A member 3 staining typeⅡ pneumocytes in mouse lung.
Panel C : ant-PSAP staining typeⅡ pneumocytes in mouse lung.
Panel D : ant-Aquaporin-5 staining typeⅠ pneumocytes in mouse lung.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324911, ab322404 and ab305303 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (AB322404)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat lung staining of Surfactant Protein A/PSAP with ab322404 at a 1/5000 (0.092 μg/ml) dilution, F4/80 with ab300421 at 1/5000 (0.070 μg/ml) dilution, and CD73 with ab314327 at 1/500 (0.98 μg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-Surfactant Protein A/PSAP (green; Opal™ 520), anti-F4/80 (grey; Opal™ 570) and anti-CD73 (magenta; Opal™ 690) on rat lung.
Panel B : anti-Surfactant Protein A/PSAP staining Clara cells and alveolar type II cells in rat lung.
Panel C : anti-F4/80 staining macrophages in rat lung.
Panel D : anti-CD73 staining epithelium in rat lung.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322404, ab300421 and ab314327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system..

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (AB322404)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lung staining of Surfactant Protein A/PSAP with ab322404 at a 1/5000 (0.092 μg/ml) dilution, F4/80 with ab300421 at 1/5000 (0.070 μg/ml) dilution, and CD73 with ab314327 at 1/500 (0.98 μg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-Surfactant Protein A/PSAP (green; Opal™ 520), anti-F4/80 (grey; Opal™ 570) and anti-CD73 (magenta; Opal™ 690) on mouse lung.
Panel B : anti-Surfactant Protein A/PSAP staining Clara cells and alveolar type II cells in mouse lung.
Panel C : anti-F4/80 staining macrophages in mouse lung.
Panel D : anti-CD73 staining epithelium in mouse lung.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322404, ab300421 and ab314327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system..

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (AB322404)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Surfactant Protein A/PSAP with ab322404 at 1/5000 (0.092 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in Clara cells and alveolar type II cells of mouse lung (PMID : 10820266).
The section was incubated with ab322404 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (AB322404)

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling Surfactant Protein A/PSAP with ab322404 at 1/5000 (0.092 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in Clara cells and alveolar type II cells of rat lung (PMID : 10820266).
The section was incubated with ab322404 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (AB322404)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : pancreas, spleen

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 15123664, PMID : 8421459).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (ab322404) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Lane 3:

Mouse pancreas tissue lysate at 20 µg

Lane 4:

Rat lung tissue lysate at 20 µg

Lane 5:

Rat spleen tissue lysate at 20 µg

Lane 6:

Rat pancreas tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 35 kDa,124 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (AB322404)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 15123664, PMID : 8421459).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (ab322404) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse SP-A expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 35 kDa,70 kDa,124 kDa

false

Exposure time: 3s