Anti-Surfactant Protein A/PSAP antibody [EPR29097-290]

8 product images

• 

  Western blot - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (ab322404)

  Surfactant Protein A/PSAP Western blot staining using rabbit Anti-Surfactant Protein A/PSAP antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15123664, PMID: 8421459).

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

  All lanes: Western blot - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (ab322404) at 1/1000 dilution

  Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag whole cell lysate at 20 µg

  Lane 2: 293T cells transfected with a mouse SP-A expression vector containing a His-tag, whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 35 kDa, 70 kDa, 124 kDa

  Exposure time: 3s

• 

  Western blot - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (ab322404)

  Surfactant Protein A/PSAP Western blot staining using rabbit Anti-Surfactant Protein A/PSAP antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Negative control: pancreas, spleen

  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:15123664, PMID: 8421459).

  In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

  All lanes: Western blot - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (ab322404) at 1/1000 dilution

  Lane 1: Mouse lung tissue lysate at 20 µg

  Lane 2: Mouse spleen tissue lysate at 20 µg

  Lane 3: Mouse pancreas tissue lysate at 20 µg

  Lane 4: Rat lung tissue lysate at 20 µg

  Lane 5: Rat spleen tissue lysate at 20 µg

  Lane 6: Rat pancreas tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 35 kDa, 124 kDa

  Exposure time: 10s

• 

  Multiplex immunohistochemistry - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (ab322404)

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat lung staining of Surfactant Protein A/PSAP with ab322404 at a 1/5000 (0.092 μg/ml) dilution, F4/80 with Anti-F4/80 antibody [EPR26545-166] ab300421 at 1/5000 (0.070 μg/ml) dilution, and CD73 with Anti-CD73 antibody [EPR28214-169] ab314327 at 1/500 (0.98 μg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

  Panel A: merged staining of anti-Surfactant Protein A/PSAP (green; Opal™ 520), anti-F4/80 (grey; Opal™ 570) and anti-CD73 (magenta; Opal™ 690) on rat lung.
  
  Panel B: anti-Surfactant Protein A/PSAP staining Clara cells and alveolar type II cells in rat lung.
  
  Panel C: anti-F4/80 staining macrophages in rat lung.
  
  Panel D: anti-CD73 staining epithelium in rat lung.
  
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of ab322404, Anti-F4/80 antibody [EPR26545-166] ab300421 and Anti-CD73 antibody [EPR28214-169] ab314327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system..

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• 

  Multiplex immunohistochemistry - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (ab322404)

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lung staining of Surfactant Protein A/PSAP with ab322404 at a 1/5000 (0.092 μg/ml) dilution, F4/80 with Anti-F4/80 antibody [EPR26545-166] ab300421 at 1/5000 (0.070 μg/ml) dilution, and CD73 with Anti-CD73 antibody [EPR28214-169] ab314327 at 1/500 (0.98 μg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

  Panel A: merged staining of anti-Surfactant Protein A/PSAP (green; Opal™ 520), anti-F4/80 (grey; Opal™ 570) and anti-CD73 (magenta; Opal™ 690) on mouse lung.
  
  Panel B: anti-Surfactant Protein A/PSAP staining Clara cells and alveolar type II cells in mouse lung.
  
  Panel C: anti-F4/80 staining macrophages in mouse lung.
  
  Panel D: anti-CD73 staining epithelium in mouse lung.
  
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of ab322404, Anti-F4/80 antibody [EPR26545-166] ab300421 and Anti-CD73 antibody [EPR28214-169] ab314327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system..

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (ab322404)

  Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Surfactant Protein A/PSAP with ab322404 at 1/5000 (0.092 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: No staining in rat spleen.
  
  The section was incubated with ab322404 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (ab322404)

  Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Surfactant Protein A/PSAP with ab322404 at 1/5000 (0.092 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: No staining in mouse spleen.
  
  The section was incubated with ab322404 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (ab322404)

  Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling Surfactant Protein A/PSAP with ab322404 at 1/5000 (0.092 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in Clara cells and alveolar type II cells of rat lung (PMID: 10820266).
  
  The section was incubated with ab322404 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] (ab322404)

  Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Surfactant Protein A/PSAP with ab322404 at 1/5000 (0.092 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining in Clara cells and alveolar type II cells of mouse lung (PMID: 10820266).
  
  The section was incubated with ab322404 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins