Rabbit Recombinant Monoclonal BIRC5 antibody. Suitable for ICC, WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Baculoviral IAP repeat-containing protein 5.
IgG
Rabbit
Preservative: 0.09% Sodium azide
Constituents: 99% PBS
Liquid
Monoclonal
ICC | WB | |
---|---|---|
Human | Tested | Tested |
Mouse | Expected | Tested |
Rat | Expected | Tested |
Cat | Predicted | Predicted |
Cow | Predicted | Predicted |
Dog | Predicted | Predicted |
Orangutan | Predicted | Predicted |
Pig | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Cat, Dog, Pig, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 2-5 µg/mL | Notes - |
Species Rat | Dilution info 2-5 µg/mL | Notes - |
Species Human | Dilution info 2-5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Cat, Dog, Pig, Orangutan | Dilution info - | Notes - |
Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:20627126, PubMed:21364656, PubMed:25778398, PubMed:28218735, PubMed:9859993). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Essential for the maintenance of mitochondrial integrity and function (PubMed:25778398). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).
API4, IAP4, API4, BIRC5, IAP4, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4, Apoptosis inhibitor survivin
Rabbit Recombinant Monoclonal BIRC5 antibody. Suitable for ICC, WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Baculoviral IAP repeat-containing protein 5.
IgG
Rabbit
Preservative: 0.09% Sodium azide
Constituents: 99% PBS
Liquid
Monoclonal
9H18L32
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This supplementary information is collated from multiple sources and compiled automatically.
Survivin 3 alpha also known as BIRC5 isoform 3 or survivin-ΔEx3 is a protein that plays a role in inhibiting apoptosis and regulating cell division. It has a molecular mass of approximately 18 kDa. This protein is expressed in various tissues but it is notably higher in cancer cells. Survivin 3 alpha is distinct in its function from the full-length survivin isoform due to its unique splice variant form which influences its mechanical role in cellular processes.
This splice variant contributes to cell cycle regulation and apoptosis inhibition. Although not extensively part of larger complexes Survivin 3 alpha can interact with microtubules during cell division assisting in the proper chromosome alignment and segregation. This activity is important for the stability and survival of rapidly dividing cells providing these cells with an adaptive advantage under stress conditions.
Survivin 3 alpha is involved in the inhibition of the apoptotic pathway and the regulation of the mitotic spindle apparatus. It interacts with important molecular players such as caspases which are pivotal in apoptosis and Aurora B kinase which is significant in regulating mitotic phase activities. Through these interactions Survivin 3 alpha modulates cell survival and mitotic processes contributing to the fine balance of cell proliferation and death.
Survivin 3 alpha expression levels are associated with cancer progression and treatment resistance. Elevated levels are commonly seen in various cancers including lung and colon cancers linking it to the same pathway network as full-length Survivin and other inhibitor of apoptosis proteins (IAPs). As a result Survivin 3 alpha participates in the mechanisms contributing to tumor survival and growth making it a target of interest for cancer therapeutics development.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Immunocytochemistry analysis of 70% confluent log phase HeLa cells labeling Survivin with ab203571 at 5 μg/mL. Cells were fixed in 4% Paraformaldehyde, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at RT. Cells were incubated overnight at RT and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1:2000 dilution for 45 minutes at RT (Panel A: green). Nuclei (Panel B: blue) were stained with DAPI. F-actin (Panel C: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1:300 dilution. Panel D is a merged image showing staining in Late Telophase. Panel E shows no primary antibody control. The images were captured at 60X magnification.
Blocking buffer: 5% skim milk.
All lanes: Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 30 µg
Lane 2: U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate at 30 µg
Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg
Lane 4: A549 (Human lung carcinoma cell line) whole cell lysate at 30 µg
Lane 5: K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 30 µg
Lane 6: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg
Lane 7: A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Lane 8: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 30 µg
All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 16 kDa
Observed band size: 17 kDa
Immunocytochemistry analysis of 70% confluent log phase HeLa cells labeling Survivin with ab203571 at 1/1000 dilution. Cells were fixed in 4% Paraformaldehyde, permeabilized with 0.25% Triton™ X-100 for 10 minutes and blocked with 5% BSA for 1 hour at RT. Cells were incubated overnight at RT and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1:400 dilution for 30 minutes at RT (Panel A: green). Nuclei (Panel B: blue) were stained with DAPI. F-actin (Panel C: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1:300 dilution. Panel D is a merged image showing nuclear localization. Panel E shows no primary antibody control. The images were captured at 20X magnification.
All lanes: Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571) at 1 µg/mL
Lane 1: HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate at 30 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 30 µg
Lane 3: PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 30 µg
Lane 4: LNCaP (Human prostate cancer cell line) whole cell lysate at 30 µg
Lane 5: A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Lane 6: SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate at 30 µg
Lane 7: SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate treated with LY294002 (10uM for 24 hours) at 30 µg
All lanes: Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 16 kDa
Observed band size: 17 kDa
Immunocytochemical analysis of 4% parafolrmaldehyde fixed MCF7 cells labeling Survivin 3 alpha using ab203571 at 5 μg/ml. Alexa Fluor® 488 Goat anti-Rabbit at 1/1000 dilution was used as secondary antibody (green). DAPI staining for nuclei (Blue).
All lanes: Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571) at 2 µg/mL
Lane 1: HeLa cell lysate at 30 µg
Lane 2: Jurkat cell lysate at 30 µg
Lane 3: A431 cell lysate at 30 µg
Lane 4: HEK293 cell lysate at 30 µg
Lane 5: Mouse kidney tissue lysate at 30 µg
Lane 6: Mouse liver tissue lysate at 30 µg
Lane 7: Mouse testis tissue lysate at 30 µg
Lane 8: Rat kidney tissue lysate at 30 µg
Lane 9: Rat testis tissue lysate at 30 µg
Predicted band size: 16 kDa
Observed band size: 17 kDa
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