Anti-Survivin 3 alpha antibody [9H18L32]

• ICC

Supplier Data

Immunocytochemistry - Anti-Survivin 3 alpha antibody [9H18L32] (AB203571)

Immunocytochemistry analysis of 70% confluent log phase HeLa cells labeling Survivin with ab203571 at 5 μg/mL. Cells were fixed in 4% Paraformaldehyde, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at RT. Cells were incubated overnight at RT and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1 : 2000 dilution for 45 minutes at RT (Panel A : green). Nuclei (Panel B : blue) were stained with DAPI. F-actin (Panel C : red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1 : 300 dilution. Panel D is a merged image showing staining in Late Telophase. Panel E shows no primary antibody control. The images were captured at 60X magnification.

• ICC

Supplier Data

Immunocytochemistry - Anti-Survivin 3 alpha antibody [9H18L32] (AB203571)

Immunocytochemical analysis of 4% parafolrmaldehyde fixed MCF7 cells labeling Survivin 3 alpha using ab203571 at 5 μg/ml. Alexa Fluor® 488 Goat anti-Rabbit at 1/1000 dilution was used as secondary antibody (green). DAPI staining for nuclei (Blue).

• ICC

Supplier Data

Immunocytochemistry - Anti-Survivin 3 alpha antibody [9H18L32] (AB203571)

Immunocytochemistry analysis of 70% confluent log phase HeLa cells labeling Survivin with ab203571 at 1/1000 dilution. Cells were fixed in 4% Paraformaldehyde, permeabilized with 0.25% Triton™ X-100 for 10 minutes and blocked with 5% BSA for 1 hour at RT. Cells were incubated overnight at RT and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1 : 400 dilution for 30 minutes at RT (Panel A : green). Nuclei (Panel B : blue) were stained with DAPI. F-actin (Panel C : red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1 : 300 dilution. Panel D is a merged image showing nuclear localization. Panel E shows no primary antibody control. The images were captured at 20X magnification.

• WB

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Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (AB203571)

Blocking buffer : 5% skim milk.

All lanes:

Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 30 µg

Lane 2:

U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate at 30 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

Lane 4:

A549 (Human lung carcinoma cell line) whole cell lysate at 30 µg

Lane 5:

K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 30 µg

Lane 6:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg

Lane 7:

A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 8:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution

Predicted band size: 16 kDa

Observed band size: 17 kDa

true

• WB

Supplier Data

Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (AB203571)

All lanes:

Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571) at 1 µg/mL

Lane 1:

HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate at 30 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 30 µg

Lane 3:

PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 30 µg

Lane 4:

LNCaP (Human prostate cancer cell line) whole cell lysate at 30 µg

Lane 5:

A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 6:

SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate at 30 µg

Lane 7:

SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate treated with LY294002 (10uM for 24 hours) at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution

Predicted band size: 16 kDa

Observed band size: 17 kDa

true

• WB

Supplier Data

Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (AB203571)

All lanes:

Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571) at 2 µg/mL

Lane 1:

HeLa cell lysate at 30 µg

Lane 2:

Jurkat cell lysate at 30 µg

Lane 3:

A431 cell lysate at 30 µg

Lane 4:

HEK293 cell lysate at 30 µg

Lane 5:

Mouse kidney tissue lysate at 30 µg

Lane 6:

Mouse liver tissue lysate at 30 µg

Lane 7:

Mouse testis tissue lysate at 30 µg

Lane 8:

Rat kidney tissue lysate at 30 µg

Lane 9:

Rat testis tissue lysate at 30 µg

Predicted band size: 16 kDa

Observed band size: 17 kDa

false