Skip to main content

Rabbit Recombinant Monoclonal BIRC5 antibody. Suitable for ICC, WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Baculoviral IAP repeat-containing protein 5.

Be the first to review this product! Submit a review

Images

Immunocytochemistry - Anti-Survivin 3 alpha antibody [9H18L32] (AB203571), expandable thumbnail
  • Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (AB203571), expandable thumbnail
  • Immunocytochemistry - Anti-Survivin 3 alpha antibody [9H18L32] (AB203571), expandable thumbnail
  • Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (AB203571), expandable thumbnail
  • Immunocytochemistry - Anti-Survivin 3 alpha antibody [9H18L32] (AB203571), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human Baculoviral IAP repeat-containing protein 5. Database link O15392

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested
Cat
Predicted
Predicted
Cow
Predicted
Predicted
Dog
Predicted
Predicted
Orangutan
Predicted
Predicted
Pig
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

5 µg/mL

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow, Cat, Dog, Pig, Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

2-5 µg/mL

Notes

-

Species

Rat

Dilution info

2-5 µg/mL

Notes

-

Species

Human

Dilution info

2-5 µg/mL

Notes

-

Predicted
Predicted

Species

Cow, Cat, Dog, Pig, Orangutan

Dilution info

-

Notes

-

Target data

Function

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:20627126, PubMed:21364656, PubMed:25778398, PubMed:28218735, PubMed:9859993). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Essential for the maintenance of mitochondrial integrity and function (PubMed:25778398). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal BIRC5 antibody. Suitable for ICC, WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Baculoviral IAP repeat-containing protein 5.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human Baculoviral IAP repeat-containing protein 5. Database link O15392
Clone number

9H18L32

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Survivin 3 alpha also known as BIRC5 isoform 3 or survivin-ΔEx3 is a protein that plays a role in inhibiting apoptosis and regulating cell division. It has a molecular mass of approximately 18 kDa. This protein is expressed in various tissues but it is notably higher in cancer cells. Survivin 3 alpha is distinct in its function from the full-length survivin isoform due to its unique splice variant form which influences its mechanical role in cellular processes.

Biological function summary

This splice variant contributes to cell cycle regulation and apoptosis inhibition. Although not extensively part of larger complexes Survivin 3 alpha can interact with microtubules during cell division assisting in the proper chromosome alignment and segregation. This activity is important for the stability and survival of rapidly dividing cells providing these cells with an adaptive advantage under stress conditions.

Pathways

Survivin 3 alpha is involved in the inhibition of the apoptotic pathway and the regulation of the mitotic spindle apparatus. It interacts with important molecular players such as caspases which are pivotal in apoptosis and Aurora B kinase which is significant in regulating mitotic phase activities. Through these interactions Survivin 3 alpha modulates cell survival and mitotic processes contributing to the fine balance of cell proliferation and death.

Associated diseases and disorders

Survivin 3 alpha expression levels are associated with cancer progression and treatment resistance. Elevated levels are commonly seen in various cancers including lung and colon cancers linking it to the same pathway network as full-length Survivin and other inhibitor of apoptosis proteins (IAPs). As a result Survivin 3 alpha participates in the mechanisms contributing to tumor survival and growth making it a target of interest for cancer therapeutics development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunocytochemistry - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571), expandable thumbnail

    Immunocytochemistry - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571)

    Immunocytochemistry analysis of 70% confluent log phase HeLa cells labeling Survivin with ab203571 at 5 μg/mL. Cells were fixed in 4% Paraformaldehyde, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at RT. Cells were incubated overnight at RT and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1:2000 dilution for 45 minutes at RT (Panel A: green). Nuclei (Panel B: blue) were stained with DAPI. F-actin (Panel C: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1:300 dilution. Panel D is a merged image showing staining in Late Telophase. Panel E shows no primary antibody control. The images were captured at 60X magnification.

  • Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571), expandable thumbnail

    Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571)

    Blocking buffer: 5% skim milk.

    All lanes: Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 30 µg

    Lane 2: U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate at 30 µg

    Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

    Lane 4: A549 (Human lung carcinoma cell line) whole cell lysate at 30 µg

    Lane 5: K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 30 µg

    Lane 6: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg

    Lane 7: A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Lane 8: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 16 kDa

    Observed band size: 17 kDa

  • Immunocytochemistry - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571), expandable thumbnail

    Immunocytochemistry - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571)

    Immunocytochemistry analysis of 70% confluent log phase HeLa cells labeling Survivin with ab203571 at 1/1000 dilution. Cells were fixed in 4% Paraformaldehyde, permeabilized with 0.25% Triton™ X-100 for 10 minutes and blocked with 5% BSA for 1 hour at RT. Cells were incubated overnight at RT and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1:400 dilution for 30 minutes at RT (Panel A: green). Nuclei (Panel B: blue) were stained with DAPI. F-actin (Panel C: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1:300 dilution. Panel D is a merged image showing nuclear localization. Panel E shows no primary antibody control. The images were captured at 20X magnification.

  • Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571), expandable thumbnail

    Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571)

    All lanes: Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571) at 1 µg/mL

    Lane 1: HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate at 30 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 30 µg

    Lane 3: PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 30 µg

    Lane 4: LNCaP (Human prostate cancer cell line) whole cell lysate at 30 µg

    Lane 5: A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Lane 6: SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate at 30 µg

    Lane 7: SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate treated with LY294002 (10uM for 24 hours) at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 16 kDa

    Observed band size: 17 kDa

  • Immunocytochemistry - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571), expandable thumbnail

    Immunocytochemistry - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571)

    Immunocytochemical analysis of 4% parafolrmaldehyde fixed MCF7 cells labeling Survivin 3 alpha using ab203571 at 5 μg/ml. Alexa Fluor® 488 Goat anti-Rabbit at 1/1000 dilution was used as secondary antibody (green). DAPI staining for nuclei (Blue).

  • Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571), expandable thumbnail

    Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571)

    All lanes: Western blot - Anti-Survivin 3 alpha antibody [9H18L32] (ab203571) at 2 µg/mL

    Lane 1: HeLa cell lysate at 30 µg

    Lane 2: Jurkat cell lysate at 30 µg

    Lane 3: A431 cell lysate at 30 µg

    Lane 4: HEK293 cell lysate at 30 µg

    Lane 5: Mouse kidney tissue lysate at 30 µg

    Lane 6: Mouse liver tissue lysate at 30 µg

    Lane 7: Mouse testis tissue lysate at 30 µg

    Lane 8: Rat kidney tissue lysate at 30 µg

    Lane 9: Rat testis tissue lysate at 30 µg

    Predicted band size: 16 kDa

    Observed band size: 17 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com