Rabbit Polyclonal BIRC5 antibody. Suitable for IHC-P, IP, Flow Cyt, ELISA, WB, RIP, ICC/IF and reacts with Human, Mouse, Rat, Dog, Cat, Cow samples. Cited in 134 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BIRC5.
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Publications
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- EMBO reports 24:e563352023Human Neuralized is a novel tumour suppressor targeting Wnt/β-catenin signalling in colon cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoo Mi Yi,Tae-Hong Kang,Yu Kyeong Han,Ha Young Park,Ju Hwan Yang,Jin-Han Bae,Jung-Soo Suh,Tae-Jin Kim,Joong-Gook Kim,Yan-Hong Cui,Hiromu Suzuki,Kohei Kumegawa,Sung Joo Kim,Yi Zhao,In Ja Park,Seung-Mo Hong,Joon-Yong Chung,Su-Jae LeePubMed 37341560
- Disease models & mechanisms 16:2023Effectiveness of irinotecan plus trabectedin on a desmoplastic small round cell tumor patient-derived xenograft.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValentina Zuco,Sandro Pasquali,Monica Tortoreto,Stefano Percio,Valentina Doldi,Marta Barisella,Paola Collini,Gian Paolo Dagrada,Silvia Brich,Patrizia Gasparini,Marco Fiore,Michela Casanova,Anna Maria Frezza,Alessandro Gronchi,Silvia Stacchiotti,Andrea Ferrari,Nadia ZaffaroniPubMed 37158111
- Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 56:e126652023A novel nano-formulation of olmesartan medoxomil with improved delivery and efficacy in the treatment of indomethacin-induced duodenitis in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesH A Murad,T M AlqurashiPubMed 37255094
- Disease markers 2022:69939942022Pygo1 Regulates the Behavior of Human Non-Small-Cell Lung Cancer via the Wnt/-Catenin Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Wen,Yuling Li,Boyu Yang,Xiangrong Guo,Li Lin,Jifeng Liang,Kai Zhang,Xu Li,Zhigang Jiang,Yuequn Wang,Yongqing Li,Xiushan Wu,Xiongwei Fan,Fang Li,Wuzhou YuanPubMed 36398031
- Experimental and therapeutic medicine 24:6142022TMEM14A aggravates the progression of human ovarian cancer cells by enhancing the activity of glycolysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingmei Zhang,Xiaohong Wang,Xuan Zhang,Jingfen Zhan,Binbin Zhang,Jin Jia,Jie ChenPubMed 36160886
- International journal of molecular sciences 23:2022Tumor-Suppressor Role of the α1-Na/K-ATPase Signalosome in NASH Related Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUtibe-Abasi S Udoh,Moumita Banerjee,Pradeep K Rajan,Juan D Sanabria,Gary Smith,Mathew Schade,Jacqueline A Sanabria,Yuto Nakafuku,Komal Sodhi,Sandrine V Pierre,Joseph I Shapiro,Juan R SanabriaPubMed 35806364
- Journal of cancer research and clinical oncology 148:2235-22462022Nuclear survivin is a prognosticator in gastroenteropancreatic neuroendocrine neoplasms: a meta-analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Krieg,Christoph Roderburg,Stephen Fung,Tom Luedde,Wolfram Trudo Knoefel,Andreas KriegPubMed 35428913
- Frontiers in oncology 11:7462662021Histone Deacetylase Inhibitor Trichostatin A Reduces Endothelial Cell Proliferation by Suppressing STAT5A-Related Gene Transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYize Li,Yongmei Zhao,Hongyan Peng,Jing Zhang,Lun Bo,Lei Wen,Wenchao Liu,Wendong Bai,Hongmei ZhangPubMed 34650929
- Pharmaceuticals (Basel, Switzerland) 14:2021miR-34a-Mediated Survivin Inhibition Improves the Antitumor Activity of Selinexor in Triple-Negative Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSilvia Martini,Valentina Zuco,Monica Tortoreto,Stefano Percio,Elisa Campi,Rihan El Bezawy,Valentina Doldi,Yosef Landesman,Marzia Pennati,Nadia ZaffaroniPubMed 34072442
- Journal of biochemical and molecular toxicology 35:e228292021Unraveling the biomechanistic role of Rac1/TWEAK/Fn14/NF-κB intricate network in experimentally doxorubicin-induced cardiotoxicity in rats: The role of curcumin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNema A Soliman,Rehab E Abo El Gheit,Muhammad T Abdel Ghafar,Norhan A AbuoHashish,Marwa A A Ibrahim,Hend S Abo Safia,Mervat H El-Saka,Amira M ElshamyPubMed 34047412
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% Tris glycine, 0.876% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human BIRC5. Database link O15392
Consider this alternative
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | ELISA | WB | RIP | ICC/IF | |
|---|---|---|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected | Tested | Expected | Tested |
| Mouse | Predicted | Expected | Expected | Expected | Predicted | Expected | Predicted |
| Rat | Predicted | Expected | Expected | Expected | Predicted | Expected | Predicted |
| Cat | Predicted | Expected | Expected | Expected | Predicted | Expected | Predicted |
| Cow | Predicted | Expected | Expected | Expected | Predicted | Expected | Predicted |
| Dog | Predicted | Expected | Expected | Expected | Predicted | Expected | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5 µg/mL | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Cat, Mouse, Cow, Rat | Dilution info - | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Recommended to use at 5-7Âμg/ml. |
Species Rat | Dilution info - | Notes Recommended to use at 5-7Âμg/ml. |
Species Human | Dilution info - | Notes Recommended to use at 5-7Âμg/ml. |
Species Dog | Dilution info - | Notes Recommended to use at 5-7Âμg/ml. |
Species Cat | Dilution info - | Notes Recommended to use at 5-7Âμg/ml. |
Species Cow | Dilution info - | Notes Recommended to use at 5-7Âμg/ml. |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Dog | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Cat | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Cow | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Dog, Cat, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Found to work at 1/5000 dilution. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Cat, Mouse, Cow, Rat | Dilution info - | Notes - |
RIP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Dog, Cat, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes See Abreview by William Moore; fix with formaldehyde. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Cat, Mouse, Cow, Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:20627126, PubMed:21364656, PubMed:25778398, PubMed:28218735, PubMed:9859993). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Essential for the maintenance of mitochondrial integrity and function (PubMed:25778398). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).
Alternative names
API4, IAP4, BIRC5, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4, Apoptosis inhibitor survivin
Recommended products
Rabbit Polyclonal BIRC5 antibody. Suitable for IHC-P, IP, Flow Cyt, ELISA, WB, RIP, ICC/IF and reacts with Human, Mouse, Rat, Dog, Cat, Cow samples. Cited in 134 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BIRC5.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% Tris glycine, 0.876% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human BIRC5. Database link O15392
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Survivin also known as 'BIRC5 protein' is a small protein with a molecular weight of approximately 16.5 kDa. It belongs to the Inhibitor of Apoptosis (IAP) family carrying out its functions through inhibiting caspases and averting apoptosis. Survivin is expressed highly in embryonic tissues and various cancers but its expression in adult differentiated tissues is low. This selective expression pattern makes Survivin an attractive target for cancer research and therapeutic development.
- Biological function summary
Survivin aids cell division and regulates apoptosis. It plays a major role as a chromosomal passenger protein functioning in concert with other members of the chromosomal passenger complex including Aurora B kinase INCENP and Borealin. During mitosis Survivin helps ensure proper chromosome alignment segregation and cytokinesis. Its ability to inhibit apoptosis allows it to enhance cell survival contributing to tumor progression when deregulated.
- Pathways
Survivin is part of the cell cycle regulation and apoptosis pathways. It plays an integral role in the mitotic spindle checkpoint working closely with Aurora B kinase to regulate mitosis. Additionally the protein interfaces with the extrinsic apoptosis pathway interacting with members like XIAP to prevent caspase activation and cell death. These interactions reveal Survivin's dual function in promoting mitosis and regulating apoptosis which is essential for maintaining cellular homeostasis.
- Associated diseases and disorders
Survivin is highly connected to cancer and neurodegenerative disorders. Its overexpression links with cancers such as colorectal cancer where it promotes malignancy through inhibiting apoptosis and ensuring unchecked cell division. In neurodegenerative disorders abnormal regulation of Survivin could lead to insufficient cellular survival contributing to diseases like Alzheimer's. The relationship between Survivin and XIAP in cancer highlights its potential as a therapeutic target aiming to induce apoptosis in cancer cells by overcoming Survivin-mediated resistance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
Western blot - Anti-Survivin antibody (ab469)
All lanes: Western blot - Anti-Survivin antibody (ab469) at 1 µg/mL
Lane 1: HeLa Nuclear at 20 µg
Lane 2: HeLa whole cell lysate at 20 µg
Lane 3: A431 cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
Lane 5: HEK293 cell lysate at 20 µg
SecondaryAll lanes: Alexa Fluor anti-rabbit at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 18 kDa, 37 kDa, 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)
Paraffin-embedded human rectal cancer tissue stained for Survivin using ab469 at 0.5 μg/ml in immunohistochemical analysis, using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Survivin (green) using ab469 at 1/10 dilution in ICC/IF. An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
This image is courtesy of a customer review submitted by Dr Ben DavidsonImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)
ab469 staining Survivin from Human Ovarian carcionoma tumour tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed (Citrate buffer pH=6, microwave oven) and the tissue was then formaldehyde fixed and blocked (Hydrogen peroxide 0.03%). An HRP conjugated goat anti-rabbit was used as the secondary antibody.
This image is courtesy of William MooreImmunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)
HeLa cells (HeLa whole cell lysate ab150035) in prometaphase, metaphase and anaphase stained with anti-Survivin (green), anti-tubulin (red) and DAPI (blue). These images were kindly supplied as part of the review submitted by William Moore, University of Dundee, UK.
Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)
ab469 at a 1/400 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then was detected using a Texas Red conjugated Goat anti-rabbit antibody.
This image is courtesy of an Abreview by Sandrine Ruchaud submitted on 30 March 2006.
Western blot - Anti-Survivin antibody (ab469)
All lanes: Western blot - Anti-Survivin antibody (ab469) at 1 µg/mL
All lanes: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 16 kDa, 18 kDa, 31 kDa, 9 kDa
Exposure time: 1min
This image is courtesy of a customer review submitted by Mr. Rudolf Jung.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)
Paraformaldehyde-fixed, paraffin-embedded human colon carcinoma tissue stained for Survivin using ab469 at 1/500 dilution in immunohistochemical analysis.
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