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Rabbit Recombinant Monoclonal BIRC5 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.


Images

Western blot - Anti-Survivin antibody [EPR20448] (AB208938), expandable thumbnail
  • Immunoprecipitation - Anti-Survivin antibody [EPR20448] (AB208938), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR20448] (AB208938), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Survivin antibody [EPR20448] (AB208938), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Survivin antibody [EPR20448] (AB208938), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:20627126, PubMed:21364656, PubMed:25778398, PubMed:28218735, PubMed:9859993). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Essential for the maintenance of mitochondrial integrity and function (PubMed:25778398). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).

Alternative names

API4, IAP4, BIRC5, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4, Apoptosis inhibitor survivin

Recommended products

Rabbit Recombinant Monoclonal BIRC5 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR20448
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Survivin also known as 'BIRC5 protein' is a small protein with a molecular weight of approximately 16.5 kDa. It belongs to the Inhibitor of Apoptosis (IAP) family carrying out its functions through inhibiting caspases and averting apoptosis. Survivin is expressed highly in embryonic tissues and various cancers but its expression in adult differentiated tissues is low. This selective expression pattern makes Survivin an attractive target for cancer research and therapeutic development.

Biological function summary

Survivin aids cell division and regulates apoptosis. It plays a major role as a chromosomal passenger protein functioning in concert with other members of the chromosomal passenger complex including Aurora B kinase INCENP and Borealin. During mitosis Survivin helps ensure proper chromosome alignment segregation and cytokinesis. Its ability to inhibit apoptosis allows it to enhance cell survival contributing to tumor progression when deregulated.

Pathways

Survivin is part of the cell cycle regulation and apoptosis pathways. It plays an integral role in the mitotic spindle checkpoint working closely with Aurora B kinase to regulate mitosis. Additionally the protein interfaces with the extrinsic apoptosis pathway interacting with members like XIAP to prevent caspase activation and cell death. These interactions reveal Survivin's dual function in promoting mitosis and regulating apoptosis which is essential for maintaining cellular homeostasis.

Associated diseases and disorders

Survivin is highly connected to cancer and neurodegenerative disorders. Its overexpression links with cancers such as colorectal cancer where it promotes malignancy through inhibiting apoptosis and ensuring unchecked cell division. In neurodegenerative disorders abnormal regulation of Survivin could lead to insufficient cellular survival contributing to diseases like Alzheimer's. The relationship between Survivin and XIAP in cancer highlights its potential as a therapeutic target aiming to induce apoptosis in cancer cells by overcoming Survivin-mediated resistance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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6 product images

  • Western blot - Anti-Survivin antibody [EPR20448] (ab208938), expandable thumbnail

    Western blot - Anti-Survivin antibody [EPR20448] (ab208938)

    Blocking/Dilution: 5% NFDM/TBST

    Exposure times: Lanes 1-2: 70 seconds; Lane 3: 48 seconds; Lane 4: 3 minutes

    All lanes: Western blot - Anti-Survivin antibody [EPR20448] (ab208938) at 1/1000 dilution

    Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 3: A549 (human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 4: Human ovary cancer whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 16 kDa

    Observed band size: 16 kDa

  • Immunoprecipitation - Anti-Survivin antibody [EPR20448] (ab208938), expandable thumbnail

    Immunoprecipitation - Anti-Survivin antibody [EPR20448] (ab208938)

    Survivin was immunoprecipitated from 0.35mg of A549 (human lung carcinoma cell line) whole cell lysate with ab208938 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab208938 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: A549 whole cell lysate 10 μg (Input).

    Lane 2: ab208938 IP in A549 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab208938 in A549 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-Survivin antibody [EPR20448] (ab208938)

    Predicted band size: 16 kDa

    Observed band size: 16 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR20448] (ab208938), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR20448] (ab208938)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Survivin with ab208938 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing kinetochores and midbody (arrow) staining in the HeLa cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/500 dilution.

  • Flow Cytometry (Intracellular) - Anti-Survivin antibody [EPR20448] (ab208938), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Survivin antibody [EPR20448] (ab208938)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A549 (human lung carcinoma cell line) cell line labeling Survivin with ab208938 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-Survivin antibody [EPR20448] (ab208938), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Survivin antibody [EPR20448] (ab208938)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Survivin with ab208938 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR20448] (ab208938), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR20448] (ab208938)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling Survivin with ab208938 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing midbody (arrow) staining in the A549 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/500 dilution.

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