Anti-Survivin antibody [EPR20448] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR20448] - BSA and Azide free (AB231076)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Survivin with ab208938 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing kinetochores and midbody (arrow) staining in the HeLa cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)  (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208938).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Survivin antibody [EPR20448] - BSA and Azide free (AB231076)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized �HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling �Survivin with ab208938 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^� 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208938).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR20448] - BSA and Azide free (AB231076)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling Survivin with ab208938 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing midbody (arrow) staining in the A549 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208938).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Survivin antibody [EPR20448] - BSA and Azide free (AB231076)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized �A549 (human lung carcinoma cell line) cell line labeling �Survivin with ab208938 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^� 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208938).

• IP

Unknown

Immunoprecipitation - Anti-Survivin antibody [EPR20448] - BSA and Azide free (AB231076)

Survivin was immunoprecipitated from 0.35mg of A549 (human lung carcinoma cell line) whole cell lysate with ab208938 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab208938 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : A549 whole cell lysate 10 μg (Input).

Lane 2 : ab208938 IP in A549 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab208938 in A549 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208938).

All lanes:

Immunoprecipitation - Anti-Survivin antibody [EPR20448] (<a href='/en-us/products/primary-antibodies/survivin-antibody-epr20448-ab208938'>ab208938</a>)

Predicted band size: 16 kDa

Observed band size: 16 kDa

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