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AB134170

Anti-Survivin antibody [EPR2675]

  • 20ul selling size
  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • What is this?

4

(5 Reviews)

|

(27 Publications)

Rabbit Recombinant Monoclonal BIRC5 antibody. Suitable for IHC-P, Flow Cyt, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 27 publications.

View Alternative Names

API4, IAP4, BIRC5, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4, Apoptosis inhibitor survivin

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] (AB134170)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] (AB134170)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling Survivin with purified ab134170 at 1/800 dilution (2.67 μg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Flow Cytometry - Anti-Survivin antibody [EPR2675] (AB134170)
  • Flow Cyt

Lab

Flow Cytometry - Anti-Survivin antibody [EPR2675] (AB134170)

Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Survivin with purified ab134170 at 1/200 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] (AB134170)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] (AB134170)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labeling Survivin with purified ab134170 at 1/800 dilution (2.67 μg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] (AB134170)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] (AB134170)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling Survivin with purified ab134170 at 1/800 dilution (2.67 μg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR2675] (AB134170)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody [EPR2675] (AB134170)

Immunocytochemistry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling Survivin with purified ab134170 at 1/200 dilution (10 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Carrier free

    Anti-Survivin antibody [EPR2675] - BSA and Azide free (Capture)

  • Carrier free

    Anti-Survivin antibody [EPR2675] - BSA and Azide free

  • 660 APC

    APC Anti-Survivin antibody [EPR2675]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Survivin antibody [EPR2675]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Survivin antibody [EPR2675]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Survivin antibody [EPR2675]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Survivin antibody [EPR2675]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Survivin antibody [EPR2675]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Survivin antibody [EPR2675]

  • HRP

    HRP Anti-Survivin antibody [EPR2675]

  • 578 PE

    PE Anti-Survivin antibody [EPR2675]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2675

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Survivin also known as 'BIRC5 protein' is a small protein with a molecular weight of approximately 16.5 kDa. It belongs to the Inhibitor of Apoptosis (IAP) family carrying out its functions through inhibiting caspases and averting apoptosis. Survivin is expressed highly in embryonic tissues and various cancers but its expression in adult differentiated tissues is low. This selective expression pattern makes Survivin an attractive target for cancer research and therapeutic development.
Biological function summary

Survivin aids cell division and regulates apoptosis. It plays a major role as a chromosomal passenger protein functioning in concert with other members of the chromosomal passenger complex including Aurora B kinase INCENP and Borealin. During mitosis Survivin helps ensure proper chromosome alignment segregation and cytokinesis. Its ability to inhibit apoptosis allows it to enhance cell survival contributing to tumor progression when deregulated.

Pathways

Survivin is part of the cell cycle regulation and apoptosis pathways. It plays an integral role in the mitotic spindle checkpoint working closely with Aurora B kinase to regulate mitosis. Additionally the protein interfaces with the extrinsic apoptosis pathway interacting with members like XIAP to prevent caspase activation and cell death. These interactions reveal Survivin's dual function in promoting mitosis and regulating apoptosis which is essential for maintaining cellular homeostasis.

Survivin is highly connected to cancer and neurodegenerative disorders. Its overexpression links with cancers such as colorectal cancer where it promotes malignancy through inhibiting apoptosis and ensuring unchecked cell division. In neurodegenerative disorders abnormal regulation of Survivin could lead to insufficient cellular survival contributing to diseases like Alzheimer's. The relationship between Survivin and XIAP in cancer highlights its potential as a therapeutic target aiming to induce apoptosis in cancer cells by overcoming Survivin-mediated resistance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed : 20627126, PubMed : 21364656, PubMed : 25778398, PubMed : 28218735, PubMed : 9859993). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed : 16322459). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed : 20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed : 20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed : 18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed : 9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed : 20826784). May play a role in neoplasia (PubMed : 10626797). Inhibitor of CASP3 and CASP7 (PubMed : 21536684). Essential for the maintenance of mitochondrial integrity and function (PubMed : 25778398). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed : 12773388, PubMed : 16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed : 10626797).
See full target information BIRC5
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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in veterinary science 12:1552415 PubMed40212948

2025

Detection of bimodal survivin expressions in canine cancer types by flow cytometry compared to immunohistochemistry.

Applications

Unspecified application

Species

Unspecified reactive species

Shohei Tsumoto,Kyoichi Tamura,Yuta Nakazawa,Michio Fujita,Kozo Ohkusu-Tsukada

Theranostics 14:5492-5511 PubMed39310097

2024

Mesenchymal stem cell-derived extracellular vesicles targeting irradiated intestine exert therapeutic effects.

Applications

Unspecified application

Species

Unspecified reactive species

Ningning He,Mingxin Dong,Yuxiao Sun,Mengmeng Yang,Yan Wang,Liqing Du,Kaihua Ji,Jinhan Wang,Manman Zhang,Yeqing Gu,Xinran Lu,Yang Liu,Qin Wang,Zongjin Li,Huijuan Song,Chang Xu,Qiang Liu

Journal of biochemical and molecular toxicology 38:e23808 PubMed39132830

2024

Calycosin activates Nrf2/Keap1 signaling to ameliorate hydrogen peroxide-induced spinal cord neuron death and mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Ruan,Ziming Cai,Zhengwen Kang,Jinzhu Liang,He Tian,Qinghe Yu,Qiusheng Zhang,Wenping Lin

Aging 16:9410-9436 PubMed38848145

2024

APOA5 alleviates reactive oxygen species to promote oxaliplatin resistance in PIK3CA-mutated colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Lin Liu,Zhuo Xiang,Bo-Ya Zhang,Yu-Wei Zou,Gui-Lai Chen,Li Yin,Yan-Long Shi,Li-Li Xu,Jingwang Bi,Qiang Wang

Cellular and molecular biology (Noisy-le-Grand, France) 70:206-210 PubMed38836659

2024

In vivo and in vitro studies on the role of regulating Smac expression in the occurrence and development of colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Donglei Zhang,Ning Yang

Cancer medicine 13:e6811 PubMed38112021

2023

BIRC5 facilitates cisplatin-chemoresistance in a mA-dependent manner in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yadan Fan,Yinglian Pan,Liping Jia,Shuzhen Gu,Binxin Liu,Ziman Mei,Chunyan Lv,Haohao Huang,Genhai Zhu,Qingchun Deng

Cancers 15: PubMed37509312

2023

NOXA Accentuates Apoptosis Induction by a Novel Histone Deacetylase Inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Ramy Ashry,Al-Hassan M Mustafa,Kristin Hausmann,Michael Linnebacher,Susanne Strand,Wolfgang Sippl,Matthias Wirth,Oliver H Krämer

Journal of advanced research 60:201-214 PubMed37467961

2023

Novel hydroxamic acid derivative induces apoptosis and constrains autophagy in leukemic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marten A Fischer,Al-Hassan M Mustafa,Kristin Hausmann,Ramy Ashry,Anita G Kansy,Magdalena C Liebl,Christina Brachetti,Andrea Piée-Staffa,Matthes Zessin,Hany S Ibrahim,Thomas G Hofmann,Mike Schutkowski,Wolfgang Sippl,Oliver H Krämer

Heliyon 9:e17101 PubMed37389063

2023

Identification of the prognostic biomarkers and their correlations with immune infiltration in colorectal cancer through bioinformatics analysis and experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Min Guo,Xiaxi Li,Jiong Li,Baolong Li

Heliyon 9:e16706 PubMed37332907

2023

Potential anticancer properties of calotropis procera: An investigation on breast and colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lara J Bou Malhab,Khuloud Bajbouj,Naglaa G Shehab,Salma M Elayoty,Jithna Sinoj,Saryia Adra,Jalal Taneera,Mohamed A Saleh,Wael M Abdel-Rahman,Mohammad H Semreen,Karem H Alzoubi,Yasser Bustanji,Waseem El-Huneidi,Eman Abu-Gharbieh
View all publications
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