Rabbit Polyclonal SUV3L1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SUPV3L1 aa 700 to C-terminus.
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Publications
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- Nucleic acids research 46:5209-52262018Polyadenylation and degradation of structurally abnormal mitochondrial tRNAs in human cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarina Toompuu,Tea Tuomela,Pia Laine,Lars Paulin,Eric Dufour,Howard T JacobsPubMed 29518244
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SUPV3L1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IYB8
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Major helicase player in mitochondrial RNA metabolism. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PubMed:29967381). ATPase and ATP-dependent multisubstrate helicase, able to unwind double-stranded (ds) DNA and RNA, and RNA/DNA heteroduplexes in the 5'-to-3' direction. Plays a role in the RNA surveillance system in mitochondria; regulates the stability of mature mRNAs, the removal of aberrantly formed mRNAs and the rapid degradation of non coding processing intermediates. Also implicated in recombination and chromatin maintenance pathways. May protect cells from apoptosis. Associates with mitochondrial DNA.
Alternative names
SUV3, SUPV3L1, Suppressor of var1 3-like protein 1, SUV3-like protein 1
Recommended products
Rabbit Polyclonal SUV3L1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SUPV3L1 aa 700 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SUPV3L1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IYB8
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab176854 was affinity purified using an epitope specific to SUV3L1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SUV3L1 also known as Suppressor of Var1 3-Like 1 is an ATP-dependent RNA helicase with a mass of approximately 90 kDa. Mechanically it unwinds RNA and participates in RNA processing and surveillance ensuring the integrity of RNA molecules. SUV3L1 exhibits a wide expression pattern being found in various tissues predominantly in regions with high mitochondrial activity due to its critical role in RNA metabolism.
- Biological function summary
The helicase activity of SUV3L1 plays an important role in mitochondrial RNA degradation. SUV3L1 forms a functional complex with polynucleotide phosphorylase (PNPase) and together they contribute to mitochondrial RNA processing and decay. This complex is vital for maintaining mitochondrial RNA homeostasis and ensuring proper mitochondrial function affecting mitochondrial biogenesis and energy production.
- Pathways
It is involved in mitochondrial gene expression and respiratory chain assembly. In the context of regulated RNA decay pathways SUV3L1 acts alongside PNPase to maintain proper levels of mitochondrial transcripts impacting cellular energy balance. Furthermore SUV3L1 is connected to the mitochondrial DNA transcription pathway where it associates with mitochondrial transcription factor B2 (TFB2M) enhancing the stability of mitochondrial transcripts and ensuring the effective production of mitochondrial proteins.
- Associated diseases and disorders
SUV3L1 has been implicated in mitochondrial disorders and certain types of cancer. Alterations in its function can lead to disrupted mitochondrial biogenesis contributing to disorders characterized by impaired mitochondrial activity. Research shows that SUV3L1 interacts with proteins like POLRMT which is part of the mitochondrial transcription machinery linking its dysregulation to mitochondrial-related dysfunctions and tumorigenesis. Understanding these associations helps illuminate SUV3L1's role in disease pathogenesis and potential therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-SUV3L1 antibody (ab176854)
All lanes: Western blot - Anti-SUV3L1 antibody (ab176854) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 88 kDa
Exposure time: 30s
Immunoprecipitation - Anti-SUV3L1 antibody (ab176854)
Detection of SUV3L1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176854 at 6 μg/mg lysate for IP (Lane 1). For WB detection, ab176854 was used at 1 μg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 1 second.
All lanes: Immunoprecipitation - Anti-SUV3L1 antibody (ab176854)
Predicted band size: 88 kDa
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