AB176854

Anti-SUV3L1 antibody

Name: Anti-SUV3L1 antibody (ab176854) | Abcam
Brand: Abcam Limited
SKU: AB176854

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(1 Publication)

Rabbit Polyclonal SUV3L1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SUPV3L1 aa 700 to C-terminus.

View Alternative Names

SUV3, SUPV3L1, Suppressor of var1 3-like protein 1, SUV3-like protein 1

2 Images

Supplier Data

Immunoprecipitation - Anti-SUV3L1 antibody (AB176854)

Detection of SUV3L1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176854 at 6 μg/mg lysate for IP (Lane 1). For WB detection, ab176854 was used at 1 μg/ml. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-SUV3L1 antibody (ab176854)

Predicted band size: 88 kDa

false

Supplier Data

Western blot - Anti-SUV3L1 antibody (AB176854)

All lanes:

Western blot - Anti-SUV3L1 antibody (ab176854) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 88 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SUPV3L1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8IYB8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab176854 was affinity purified using an epitope specific to SUV3L1 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SUV3L1 also known as Suppressor of Var1 3-Like 1 is an ATP-dependent RNA helicase with a mass of approximately 90 kDa. Mechanically it unwinds RNA and participates in RNA processing and surveillance ensuring the integrity of RNA molecules. SUV3L1 exhibits a wide expression pattern being found in various tissues predominantly in regions with high mitochondrial activity due to its critical role in RNA metabolism.

Biological function summary

The helicase activity of SUV3L1 plays an important role in mitochondrial RNA degradation. SUV3L1 forms a functional complex with polynucleotide phosphorylase (PNPase) and together they contribute to mitochondrial RNA processing and decay. This complex is vital for maintaining mitochondrial RNA homeostasis and ensuring proper mitochondrial function affecting mitochondrial biogenesis and energy production.

Pathways

It is involved in mitochondrial gene expression and respiratory chain assembly. In the context of regulated RNA decay pathways SUV3L1 acts alongside PNPase to maintain proper levels of mitochondrial transcripts impacting cellular energy balance. Furthermore SUV3L1 is connected to the mitochondrial DNA transcription pathway where it associates with mitochondrial transcription factor B2 (TFB2M) enhancing the stability of mitochondrial transcripts and ensuring the effective production of mitochondrial proteins.

SUV3L1 has been implicated in mitochondrial disorders and certain types of cancer. Alterations in its function can lead to disrupted mitochondrial biogenesis contributing to disorders characterized by impaired mitochondrial activity. Research shows that SUV3L1 interacts with proteins like POLRMT which is part of the mitochondrial transcription machinery linking its dysregulation to mitochondrial-related dysfunctions and tumorigenesis. Understanding these associations helps illuminate SUV3L1's role in disease pathogenesis and potential therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Major helicase player in mitochondrial RNA metabolism. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PubMed : 29967381). ATPase and ATP-dependent multisubstrate helicase, able to unwind double-stranded (ds) DNA and RNA, and RNA/DNA heteroduplexes in the 5'-to-3' direction. Plays a role in the RNA surveillance system in mitochondria; regulates the stability of mature mRNAs, the removal of aberrantly formed mRNAs and the rapid degradation of non coding processing intermediates. Also implicated in recombination and chromatin maintenance pathways. May protect cells from apoptosis. Associates with mitochondrial DNA.

See full target information SUPV3L1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 46:5209-5226 PubMed29518244

2018

Polyadenylation and degradation of structurally abnormal mitochondrial tRNAs in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Toompuu,Tea Tuomela,Pia Laine,Lars Paulin,Eric Dufour,Howard T Jacobs

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SUV3L1 antibody

Immunoprecipitation - Anti-SUV3L1 antibody (AB176854)

Western blot - Anti-SUV3L1 antibody (AB176854)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise