Skip to main content

Knockout Tested Rabbit Polyclonal SUZ12 antibody. Suitable for IHC-P, ChIP/Chip, IP, ChIP, WB, RIP and reacts with Common marmoset, Human, Mouse samples. Cited in 146 publications.


Images

Western blot - Anti-SUZ12 antibody (AB12073), expandable thumbnail
  • Western blot - Anti-SUZ12 antibody (AB12073), expandable thumbnail
  • Western blot - Anti-SUZ12 antibody (AB12073), expandable thumbnail
  • Western blot - Anti-SUZ12 antibody (AB12073), expandable thumbnail
  • Western blot - Anti-SUZ12 antibody (AB12073), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PChIP/ChipIPChIPWBRIP
Human
Expected
Expected
Expected
Expected
Tested
Expected
Mouse
Expected
Expected
Expected
Expected
Predicted
Expected
Common marmoset
Expected
Expected
Expected
Expected
Predicted
Expected

Expected
Expected

Species

Common marmoset, Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Mouse, Common marmoset, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Mouse, Common marmoset, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Mouse, Common marmoset, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1-5 µg/mL

Notes

-

Predicted
Predicted

Species

Common marmoset, Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Common marmoset, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Polycomb group (PcG) protein. Component of the PRC2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene (PubMed:15225548, PubMed:15231737, PubMed:15385962, PubMed:16618801, PubMed:17344414, PubMed:18285464, PubMed:28229514, PubMed:29499137, PubMed:31959557). The PRC2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (PubMed:12351676, PubMed:12435631, PubMed:15099518, PubMed:15225548, PubMed:15385962, PubMed:15684044, PubMed:16431907, PubMed:18086877, PubMed:18285464). Genes repressed by the PRC2 complex include HOXC8, HOXA9, MYT1 and CDKN2A (PubMed:15231737, PubMed:16618801, PubMed:17200670, PubMed:31959557).

Alternative names

Recommended products

Knockout Tested Rabbit Polyclonal SUZ12 antibody. Suitable for IHC-P, ChIP/Chip, IP, ChIP, WB, RIP and reacts with Common marmoset, Human, Mouse samples. Cited in 146 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-SUZ12 antibody (ab12073), expandable thumbnail

    Western blot - Anti-SUZ12 antibody (ab12073)

    Lanes 1 - 4: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

    ab12073 was shown to react with SUZ12 in western blot. The band observed in the SUZ12 knockout cell line Human SUZ12 knockout HeLa cell line ab264983 (SUZ12 knockout cell lysate Human SUZ12 knockout HeLa cell lysate ab257721) lane below 95 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab12073 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-SUZ12 antibody (ab12073) at 1 µg/mL

    Lane 1: Wild-type Hela cell lysate at 20 µg

    Lane 2: SUZ12 knockout HeLa cell lysate at 20 µg

    Lane 3: Wild-type HAP1 cell lysate at 20 µg

    Lane 4: SUZ12 knockout HAP1 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 83 kDa

    Observed band size: 95 kDa

  • Western blot - Anti-SUZ12 antibody (ab12073), expandable thumbnail

    Western blot - Anti-SUZ12 antibody (ab12073)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: SUZ12 knockout HAP1 cell lysate (20 μg)
    Lane 3: Caco2 cell lysate (20 μg)
    Lane 4: MCF7 cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab12073 was shown to specifically recognize SUZ12 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when SUZ12 knockout samples were used. Wild-type and SUZ12 knockout samples were subjected to SDS-PAGE. ab12073 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted to 1μg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-SUZ12 antibody (ab12073)

    Predicted band size: 83 kDa

    Observed band size: 95 kDa

  • Western blot - Anti-SUZ12 antibody (ab12073), expandable thumbnail

    Western blot - Anti-SUZ12 antibody (ab12073)

    Lanes 1- 2: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab12073 was shown to react with SUZ12 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line Human SUZ12 knockout HeLa cell line ab264983 (CRISPR/Cas9 edited cell lysate Human SUZ12 knockout HeLa cell lysate ab257721) lane below 95kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and SUZ12 CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab12073 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 μg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SUZ12 antibody (ab12073)

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: SUZ12 CRISPR/Cas9 edited HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 83 kDa

    Observed band size: 95 kDa

  • Western blot - Anti-SUZ12 antibody (ab12073), expandable thumbnail

    Western blot - Anti-SUZ12 antibody (ab12073)

    All lanes: Western blot - Anti-SUZ12 antibody (ab12073) at 1 µg/mL

    Lane 1: untransfected 293T cell lysate at 20 µg

    Lane 2: 293T cells transfected with 5ug HA-Suz12 at 20 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG H&L (HRP) (Dako) at 1/2000 dilution

    Performed under reducing conditions.

    Predicted band size: 83 kDa

    Observed band size: 76 kDa, 95 kDa

  • Western blot - Anti-SUZ12 antibody (ab12073), expandable thumbnail

    Western blot - Anti-SUZ12 antibody (ab12073)

    All lanes: Western blot - Anti-SUZ12 antibody (ab12073) at 1 µg/mL

    Lane 1: SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 20 µg

    Lane 2: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg

    Lane 3: Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 83 kDa

    Observed band size: 30 kDa, 53 kDa, 60 kDa, 75 kDa, 95 kDa

    Exposure time: 4min

  • Western blot - Anti-SUZ12 antibody (ab12073), expandable thumbnail

    Western blot - Anti-SUZ12 antibody (ab12073)

    Lanes 1- 2: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab12073 was shown to react with SUZ12 in wild-type HeLa cells in western blot. The band observed in knockout cell line Human SUZ12 knockout HeLa cell line ab264983 (knockout cell lysate Human SUZ12 knockout HeLa cell lysate ab257721) lane below 95kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and SUZ12 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab12073 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 μg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SUZ12 antibody (ab12073)

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: SUZ12 knockout HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 83 kDa

    Observed band size: 95 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com