Knockout Tested Rabbit Polyclonal SUZ12 antibody. Suitable for IHC-P, ChIP/Chip, IP, ChIP, WB, RIP and reacts with Common marmoset, Human, Mouse samples. Cited in 146 publications.
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- Oncogene 42:994-10092023Dissecting and targeting noncanonical functions of EZH2 in multiple myeloma via an EZH2 degrader.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXufen Yu,Jun Wang,Weida Gong,Anqi Ma,Yudao Shen,Chengwei Zhang,Xijuan Liu,Ling Cai,Jing Liu,Gang Greg Wang,Jian JinPubMed 36747009
- Nucleic acids research 50:10929-109462022A cryptic transactivation domain of EZH2 binds AR and AR's splice variant, promoting oncogene activation and tumorous transformation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Wang,Kwang-Su Park,Xufen Yu,Weida Gong,H Shelton Earp,Gang Greg Wang,Jian Jin,Ling CaiPubMed 36300627
- Cell reports 40:1111362022HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng-Chih Kuo,Matt J Neville,Rugivan Sabaratnam,Agata Wesolowska-Andersen,Daniel Phillips,Laura B L Wittemans,Andrea D van Dam,Nellie Y Loh,Marijana Todorčević,Nathan Denton,Katherine A Kentistou,Peter K Joshi,Constantinos Christodoulides,Claudia Langenberg,Philippe Collas,Fredrik Karpe,Katherine E PinnickPubMed 35905723
- Molecular cancer 21:1502022MARCKSL1-2 reverses docetaxel-resistance of lung adenocarcinoma cells by recruiting SUZ12 to suppress HDAC1 and elevate miR-200b.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Jiang,Feng Qi,Kai Zhang,Xiaofei Zhang,Jingjing Ma,Suhua Xia,Longbang Chen,Zhengyuan Yu,Jing Chen,Dongqin ChenPubMed 35864549
- Nature communications 13:34932022ZFP281-BRCA2 prevents R-loop accumulation during DNA replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Wang,Binbin Ma,Xiaoxu Liu,Ge Gao,Zhuanzhuan Che,Menghan Fan,Siyan Meng,Xiru Zhao,Rio Sugimura,Hua Cao,Zhongjun Zhou,Jing Xie,Chengqi Lin,Zhuojuan LuoPubMed 35715464
- Cell reports 39:1109282022A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Huang,Nazym Bashkenova,Yantao Hong,Cong Lyu,Diana Guallar,Zhe Hu,Vikas Malik,Dan Li,Hailin Wang,Xiaohua Shen,Hongwei Zhou,Jianlong WangPubMed 35675764
- Cell reports 39:1108302022Xist-mediated silencing requires additive functions of SPEN and Polycomb together with differentiation-dependent recruitment of SmcHD1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph S Bowness,Tatyana B Nesterova,Guifeng Wei,Lisa Rodermund,Mafalda Almeida,Heather Coker,Emma J Carter,Artun Kadaster,Neil BrockdorffPubMed 35584662
- iScience 25:1036802022An adipose lncRAP2-Igf2bp2 complex enhances adipogenesis and energy expenditure by stabilizing target mRNAs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan R Alvarez-Dominguez,Sally Winther,Jacob B Hansen,Harvey F Lodish,Marko KnollPubMed 35036870
- Blood cancer discovery 3:116-1352022BCOR and BCORL1 Mutations Drive Epigenetic Reprogramming and Oncogenic Signaling by Unlinking PRC1.1 from Target Genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEva J Schaefer,Helen C Wang,Hannah Q Karp,Clifford A Meyer,Paloma Cejas,Micah D Gearhart,Emmalee R Adelman,Iman Fares,Annie Apffel,Klothilda Lim,Yingtian Xie,Christopher J Gibson,Monica Schenone,H Moses Murdock,Eunice S Wang,Lukasz P Gondek,Martin P Carroll,Rahul S Vedula,Eric S Winer,Jacqueline S Garcia,Richard M Stone,Marlise R Luskin,Steven A Carr,Henry W Long,Vivian J Bardwell,Maria E Figueroa,R Coleman LindsleyPubMed 35015684
- Molecules (Basel, Switzerland) 26:2021Transcriptomics-Based Repositioning of Natural Compound, Eudesmin, as a PRC2 Modulator.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSang Ah Yi,Ki Hong Nam,Min Gyu Lee,Hwamok Oh,Jae Sung Noh,Jae Kyun Jeong,Sangwoo Kwak,Ye Ji Jeon,So Hee Kwon,Jaecheol Lee,Jeung-Whan HanPubMed 34577136
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IHC-P | ChIP/Chip | IP | ChIP | WB | RIP | |
|---|---|---|---|---|---|---|
| Human | Expected | Expected | Expected | Expected | Tested | Expected |
| Mouse | Expected | Expected | Expected | Expected | Predicted | Expected |
| Common marmoset | Expected | Expected | Expected | Expected | Predicted | Expected |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Common marmoset, Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ChIP/Chip
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Common marmoset, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Common marmoset, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ChIP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Common marmoset, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Common marmoset, Mouse | Dilution info - | Notes - |
RIP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Common marmoset, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Polycomb group (PcG) protein. Component of the PRC2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene (PubMed:15225548, PubMed:15231737, PubMed:15385962, PubMed:16618801, PubMed:17344414, PubMed:18285464, PubMed:28229514, PubMed:29499137, PubMed:31959557). The PRC2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (PubMed:12351676, PubMed:12435631, PubMed:15099518, PubMed:15225548, PubMed:15385962, PubMed:15684044, PubMed:16431907, PubMed:18086877, PubMed:18285464). Genes repressed by the PRC2 complex include HOXC8, HOXA9, MYT1 and CDKN2A (PubMed:15231737, PubMed:16618801, PubMed:17200670, PubMed:31959557).
Alternative names
CHET9, JJAZ1, KIAA0160, SUZ12, Polycomb protein SUZ12, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein, Suppressor of zeste 12 protein homolog, ChET 9 protein
Recommended products
Knockout Tested Rabbit Polyclonal SUZ12 antibody. Suitable for IHC-P, ChIP/Chip, IP, ChIP, WB, RIP and reacts with Common marmoset, Human, Mouse samples. Cited in 146 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
Western blot - Anti-SUZ12 antibody (ab12073)
Lanes 1 - 4: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.ab12073 was shown to react with SUZ12 in western blot. The band observed in the SUZ12 knockout cell line Human SUZ12 knockout HeLa cell line ab264983 (SUZ12 knockout cell lysate Human SUZ12 knockout HeLa cell lysate ab257721) lane below 95 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab12073 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-SUZ12 antibody (ab12073) at 1 µg/mL
Lane 1: Wild-type Hela cell lysate at 20 µg
Lane 2: SUZ12 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human SUZ12 knockout HeLa cell line (Human SUZ12 knockout HeLa cell line ab264983)
Lane 3: Wild-type HAP1 cell lysate at 20 µg
Lane 4: SUZ12 knockout HAP1 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 95 kDa
Western blot - Anti-SUZ12 antibody (ab12073)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: SUZ12 knockout HAP1 cell lysate (20 μg)
Lane 3: Caco2 cell lysate (20 μg)
Lane 4: MCF7 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.ab12073 was shown to specifically recognize SUZ12 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when SUZ12 knockout samples were used. Wild-type and SUZ12 knockout samples were subjected to SDS-PAGE. ab12073 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted to 1μg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-SUZ12 antibody (ab12073)
Predicted band size: 83 kDa
Observed band size: 95 kDa
Western blot - Anti-SUZ12 antibody (ab12073)
Lanes 1- 2: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.ab12073 was shown to react with SUZ12 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line Human SUZ12 knockout HeLa cell line ab264983 (CRISPR/Cas9 edited cell lysate Human SUZ12 knockout HeLa cell lysate ab257721) lane below 95kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and SUZ12 CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab12073 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 μg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-SUZ12 antibody (ab12073)
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: SUZ12 CRISPR/Cas9 edited HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 95 kDa
Western blot - Anti-SUZ12 antibody (ab12073)
All lanes: Western blot - Anti-SUZ12 antibody (ab12073) at 1 µg/mL
Lane 1: untransfected 293T cell lysate at 20 µg
Lane 2: 293T cells transfected with 5ug HA-Suz12 at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG H&L (HRP) (Dako) at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 76 kDa, 95 kDa
Western blot - Anti-SUZ12 antibody (ab12073)
All lanes: Western blot - Anti-SUZ12 antibody (ab12073) at 1 µg/mL
Lane 1: SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg
Lane 3: Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 30 kDa, 53 kDa, 60 kDa, 75 kDa, 95 kDa
Exposure time: 4min
Western blot - Anti-SUZ12 antibody (ab12073)
Lanes 1- 2: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.ab12073 was shown to react with SUZ12 in wild-type HeLa cells in western blot. The band observed in knockout cell line Human SUZ12 knockout HeLa cell line ab264983 (knockout cell lysate Human SUZ12 knockout HeLa cell lysate ab257721) lane below 95kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and SUZ12 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab12073 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 μg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-SUZ12 antibody (ab12073)
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: SUZ12 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 95 kDa
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