Knockout Tested Rabbit Polyclonal SUZ12 antibody. Suitable for IHC-P, ChIP/Chip, IP, ChIP, WB, RIP and reacts with Common marmoset, Human, Mouse samples. Cited in 146 publications.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
IHC-P | ChIP/Chip | IP | ChIP | WB | RIP | |
---|---|---|---|---|---|---|
Human | Expected | Expected | Expected | Expected | Tested | Expected |
Mouse | Expected | Expected | Expected | Expected | Predicted | Expected |
Common marmoset | Expected | Expected | Expected | Expected | Predicted | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Common marmoset, Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Common marmoset, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Common marmoset, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Common marmoset, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1-5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Common marmoset, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Common marmoset, Human | Dilution info Use at an assay dependent concentration. | Notes - |
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Polycomb group (PcG) protein. Component of the PRC2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene (PubMed:15225548, PubMed:15231737, PubMed:15385962, PubMed:16618801, PubMed:17344414, PubMed:18285464, PubMed:28229514, PubMed:29499137, PubMed:31959557). The PRC2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (PubMed:12351676, PubMed:12435631, PubMed:15099518, PubMed:15225548, PubMed:15385962, PubMed:15684044, PubMed:16431907, PubMed:18086877, PubMed:18285464). Genes repressed by the PRC2 complex include HOXC8, HOXA9, MYT1 and CDKN2A (PubMed:15231737, PubMed:16618801, PubMed:17200670, PubMed:31959557).
CHET9, JJAZ1, KIAA0160, SUZ12, KIAA0160, JJAZ1, CHET9, Polycomb protein SUZ12, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein, Suppressor of zeste 12 protein homolog, ChET 9 protein
Knockout Tested Rabbit Polyclonal SUZ12 antibody. Suitable for IHC-P, ChIP/Chip, IP, ChIP, WB, RIP and reacts with Common marmoset, Human, Mouse samples. Cited in 146 publications.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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Terms & Conditions.
Lanes 1 - 4: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab12073 was shown to react with SUZ12 in western blot. The band observed in the SUZ12 knockout cell line Human SUZ12 knockout HeLa cell line ab264983 (SUZ12 knockout cell lysate Human SUZ12 knockout HeLa cell lysate ab257721) lane below 95 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab12073 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-SUZ12 antibody (ab12073) at 1 µg/mL
Lane 1: Wild-type Hela cell lysate at 20 µg
Lane 2: SUZ12 knockout HeLa cell lysate at 20 µg
Lane 3: Wild-type HAP1 cell lysate at 20 µg
Lane 4: SUZ12 knockout HAP1 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 95 kDa
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: SUZ12 knockout HAP1 cell lysate (20 μg)
Lane 3: Caco2 cell lysate (20 μg)
Lane 4: MCF7 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab12073 was shown to specifically recognize SUZ12 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when SUZ12 knockout samples were used. Wild-type and SUZ12 knockout samples were subjected to SDS-PAGE. ab12073 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted to 1μg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-SUZ12 antibody (ab12073)
Predicted band size: 83 kDa
Observed band size: 95 kDa
Lanes 1- 2: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
ab12073 was shown to react with SUZ12 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line Human SUZ12 knockout HeLa cell line ab264983 (CRISPR/Cas9 edited cell lysate Human SUZ12 knockout HeLa cell lysate ab257721) lane below 95kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and SUZ12 CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab12073 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 μg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-SUZ12 antibody (ab12073)
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: SUZ12 CRISPR/Cas9 edited HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 95 kDa
All lanes: Western blot - Anti-SUZ12 antibody (ab12073) at 1 µg/mL
Lane 1: untransfected 293T cell lysate at 20 µg
Lane 2: 293T cells transfected with 5ug HA-Suz12 at 20 µg
All lanes: Goat polyclonal to Rabbit IgG H&L (HRP) (Dako) at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 76 kDa, 95 kDa
All lanes: Western blot - Anti-SUZ12 antibody (ab12073) at 1 µg/mL
Lane 1: SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg
Lane 3: Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 30 kDa, 53 kDa, 60 kDa, 75 kDa, 95 kDa
Exposure time: 4min
Lanes 1- 2: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
ab12073 was shown to react with SUZ12 in wild-type HeLa cells in western blot. The band observed in knockout cell line Human SUZ12 knockout HeLa cell line ab264983 (knockout cell lysate Human SUZ12 knockout HeLa cell lysate ab257721) lane below 95kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and SUZ12 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab12073 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 μg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-SUZ12 antibody (ab12073)
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: SUZ12 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 95 kDa
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