AB236322

Anti-SUZ12 antibody

Name: Anti-SUZ12 antibody (ab236322) | Abcam
Brand: Abcam Limited
SKU: AB236322
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal SUZ12 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SUZ12 aa 100-350.

View Alternative Names

CHET9, JJAZ1, KIAA0160, SUZ12, Polycomb protein SUZ12, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein, Suppressor of zeste 12 protein homolog, ChET 9 protein

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-SUZ12 antibody (AB236322)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SUZ12 antibody (AB236322)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SUZ12 (green) using ab236322 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody (AB236322)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody (AB236322)

Paraffin-embedded human kidney tissue stained for SUZ12 using ab236322 at 1/1000 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

Supplier Data

Western blot - Anti-SUZ12 antibody (AB236322)

All lanes:

Western blot - Anti-SUZ12 antibody (ab236322) at 1/500 dilution

Lane 1:

Rat kidney lysate

Lane 2:

Rat liver lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 83 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SUZ12 aa 100-350. The exact immunogen used to generate this antibody is proprietary information.

Q15022

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Polycomb group (PcG) protein. Component of the PRC2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene (PubMed : 15225548, PubMed : 15231737, PubMed : 15385962, PubMed : 16618801, PubMed : 17344414, PubMed : 18285464, PubMed : 28229514, PubMed : 29499137, PubMed : 31959557). The PRC2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (PubMed : 12351676, PubMed : 12435631, PubMed : 15099518, PubMed : 15225548, PubMed : 15385962, PubMed : 15684044, PubMed : 16431907, PubMed : 18086877, PubMed : 18285464). Genes repressed by the PRC2 complex include HOXC8, HOXA9, MYT1 and CDKN2A (PubMed : 15231737, PubMed : 16618801, PubMed : 17200670, PubMed : 31959557).

See full target information SUZ12

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 18:197 PubMed34511122

2021

LncRNA HOXA-AS2 regulates microglial polarization via recruitment of PRC2 and epigenetic modification of PGC-1α expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodong Yang,Yi Zhang,Yimeng Chen,Xiaoqin He,Yiwei Qian,Shaoqing Xu,Chao Gao,Chengjun Mo,Shengdi Chen,Qin Xiao

Journal of Cancer 10:1375-1384 PubMed31031847

2019

The Down-Regulation of SUZ12 Accelerates the Migration and Invasion of Liver Cancer Cells via Activating ERK1/2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Cailin Xue,Kunyuan Wang,Xiaofeng Jiang,Chengxin Gu,Ganxiang Yu,Yun Zhong,Shiming Liu,Yuqiang Nie,Yongjian Zhou,Hui Yang

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