Mouse Monoclonal SUZ12 antibody. Suitable for Flow Cyt, WB, ICC/IF, IHC-Fr, IHC-P and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SUZ12.
IgG1
Mouse
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Liquid
Monoclonal
Flow Cyt | WB | ICC/IF | IHC-Fr | IHC-P | |
---|---|---|---|---|---|
Human | Tested | Tested | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.1 µg for 106 Cells | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/15 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/15 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Polycomb group (PcG) protein. Component of the PRC2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene (PubMed:15225548, PubMed:15231737, PubMed:15385962, PubMed:16618801, PubMed:17344414, PubMed:18285464, PubMed:28229514, PubMed:29499137, PubMed:31959557). The PRC2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (PubMed:12351676, PubMed:12435631, PubMed:15099518, PubMed:15225548, PubMed:15385962, PubMed:15684044, PubMed:16431907, PubMed:18086877, PubMed:18285464). Genes repressed by the PRC2 complex include HOXC8, HOXA9, MYT1 and CDKN2A (PubMed:15231737, PubMed:16618801, PubMed:17200670, PubMed:31959557).
CHET9, JJAZ1, KIAA0160, SUZ12, KIAA0160, JJAZ1, CHET9, Polycomb protein SUZ12, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein, Suppressor of zeste 12 protein homolog, ChET 9 protein
Mouse Monoclonal SUZ12 antibody. Suitable for Flow Cyt, WB, ICC/IF, IHC-Fr, IHC-P and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SUZ12.
IgG1
Mouse
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Liquid
Monoclonal
SUZ220A
Tissue culture supernatant
Blue Ice
+4°C
+4°C
Do Not Freeze
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Overlay histogram showing MCF7 cells stained with ab126577 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126577, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Note: A purified version of the antibody was used at 1/100 (stock solution at 1mg/ml).
All lanes: Western blot - Anti-SUZ12 antibody [SUZ220A] (ab126577) at 1/100 dilution
Lane 1: Hek-SUZ12-V5 lysate at 30 µg
Lane 2: Hek-mTIMP2 lysate at 30 µg
Lane 3: Hela cell line lysate at 200 µg
Lane 4: Jeko1 cell line lysate at 200 µg
Lane 5: Human tonsil lysate at 200 µg
Lane 6: Human brain lysate at 200 µg
Lane 7: MT-2 cell line lysate at 200 µg
Lane 8: 3T3 Mouse cell line lysate at 200 µg
Lane 9: U266 cell line lysate at 200 µg
Predicted band size: 83 kDa
Observed band size: 83 kDa
Effect of SUZ12 RNA interference (siRNA) in Jeko-1 and Z-138 mantle cell lymphoma cell lines treated with two different hairpins against SUZ12 during three days. A control cell line (empty vector) was used as negative control. Expression of SUZ12 was analyzed using ab126577. A decrease of protein expression was observed in siSUZ12 Jeko-1 and Z-138 cell extracts confirming antibody specificity. Band signals were normalized with tubulin as a loading control.
All lanes: Western blot - Anti-SUZ12 antibody [SUZ220A] (ab126577)
Lanes 1 and 4: Empty vector
Lanes 2 and 5: shSUZ12.783
Lanes 3 and 6: shSUZ12.2076
Predicted band size: 83 kDa
Observed band size: 83 kDa
Purified version of ab126577, at 1/500, staining SUZ12 in frozen Human tonsil tissue by Immunohistochemistry.
ab126577, at a 1/30 dilution, staining SUZ12 in paraffin embedded Mouse testicle tissue by Immunohistochemistry.
Purified version of ab126577, at a 1/500 dilution, staining SUZ12 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
Purified version of ab126577, at a 1/500 dilution, staining SUZ12 in paraffin embedded Human testicle tissue by Immunohistochemistry.
Purified ab126577 at 1/500 staining SUZ12 in paraffin embedded Human thymus tissue by immunohistochemistry.
Purified version of ab126577, at a 1/100 dilution, staining SUZ12 (green) in Human tonsil by Immunofluorescence. IgD antibody (red) was used at 1/50. After antigen retrieval (Tris-EDTA buffer), the slides were incubated for one hour at room temperature in a humid chamber with primary antibodies. Slides were then washed in PBS 0.5%-Tween20 3 times for 5 minutes each. The slides were incubated for 1 hour with fluorochrome-conjugated antibodies against the different Ig isotypes, diluted in PBS (dilution 1/200) in a humid chamber in the dark. Subsequently, slides were washed in PBS 0.5% Tween20 3 times for 5 minutes each. Following washing, antifading was added.
To confirm that ab126577 recognized Human SUZ12 protein, Immunohistochemistry on frozen cytospins preparations of V5-tagged Human SUZ12 expressed in Hek293T was performed. Labeling with the ab126577 confirmed the efficiency of transfection. Cytospin preparation of V5-tagged Human BTLA protein was used as a negative control.
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