AB224073

Anti-SV2A antibody

Name: Anti-SV2A antibody (ab224073) | Abcam
Brand: Abcam Limited
SKU: AB224073
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal SV2A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SV2A aa 50-200.

View Alternative Names

KIAA0736, PSEC0174, SV2A, Synaptic vesicle glycoprotein 2A

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody (AB224073)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody (AB224073)

Paraffin-embedded human cerebral cortex tissue stained for SV2A with ab224073 (1/50 dilution) in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SV2A antibody (AB224073)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SV2A antibody (AB224073)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for SV2A (green) using ab224073 (4 μg/ml) in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody (AB224073)

Paraffin-embedded human cerebellum tissue stained for SV2A with ab224073 (1/50 dilution) in immunohistochemical analysis.

Unknown

Western blot - Anti-SV2A antibody (AB224073)

All lanes:

Western blot - Anti-SV2A antibody (ab224073)

All lanes:

Cerebral cortex tissue

Predicted band size: 83 kDa

false

Unknown

Western blot - Anti-SV2A antibody (AB224073)

Loading control : Anti-GAPDH

Lane 1:

ab224073 at 0.4 µg/mL

Lane 2:

Western blot - Anti-SV2A antibody (ab224073) at 0.4 µg/mL

Lane 1:

U-2 OS (Human bone osteosarcoma epithelial cells) cell line

Lane 2:

PC-3 (Human prostate cancer cells) cell line

Predicted band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SV2A aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q7L0J3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles (By similarity).. (Microbial infection) Receptor for the C.botulinum neurotoxin type A2 (BoNT/A, botA); glycosylation is not essential but enhances the interaction (PubMed : 29649119). Probably also serves as a receptor for the closely related C.botulinum neurotoxin type A1.

See full target information SV2A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 14:e0213751 PubMed30856233

2019

Development of a high-throughput fluorescent no-wash sodium influx assay.

Applications

Unspecified application

Species

Unspecified reactive species

Bryan Tay,Teneale A Stewart,Felicity M Davis,Jennifer R Deuis,Irina Vetter

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SV2A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody (AB224073)

Immunocytochemistry/ Immunofluorescence - Anti-SV2A antibody (AB224073)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody (AB224073)

Western blot - Anti-SV2A antibody (AB224073)

Western blot - Anti-SV2A antibody (AB224073)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise