Rabbit Polyclonal SV2A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SV2A aa 50-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SV2A aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q7L0J3
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Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Cow | Predicted | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04-0.4 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25-2 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles (By similarity). (Microbial infection) Receptor for the C.botulinum neurotoxin type A2 (BoNT/A, botA); glycosylation is not essential but enhances the interaction (PubMed:29649119). Probably also serves as a receptor for the closely related C.botulinum neurotoxin type A1.
Alternative names
KIAA0736, PSEC0174, SV2A, Synaptic vesicle glycoprotein 2A
Recommended products
Rabbit Polyclonal SV2A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SV2A aa 50-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SV2A aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q7L0J3
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody (ab224073)
Paraffin-embedded human cerebellum tissue stained for SV2A with ab224073 (1/50 dilution) in immunohistochemical analysis.
Western blot - Anti-SV2A antibody (ab224073)
All lanes: Western blot - Anti-SV2A antibody (ab224073)
All lanes: Cerebral cortex tissue
Predicted band size: 83 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SV2A antibody (ab224073)
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for SV2A (green) using ab224073 (4 μg/ml) in ICC/IF.
Western blot - Anti-SV2A antibody (ab224073)
Loading control: Anti-GAPDH
Lane 1: ab224073 at 0.4 µg/mL
Lane 2: Western blot - Anti-SV2A antibody (ab224073) at 0.4 µg/mL
Lane 1: U-2 OS (Human bone osteosarcoma epithelial cells) cell line
Lane 2: PC-3 (Human prostate cancer cells) cell line
Predicted band size: 83 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody (ab224073)
Paraffin-embedded human cerebral cortex tissue stained for SV2A with ab224073 (1/50 dilution) in immunohistochemical analysis.
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