AB32942

Anti-SV2A antibody

Name: Anti-SV2A antibody (ab32942) | Abcam
Brand: Abcam Limited
SKU: AB32942
Price: 460 USD
Availability: InStock
Rating: 4 (8 reviews)

(8 Reviews)

(45 Publications)

Anti-SV2A antibody (ab32942) is a rabbit polyclonal antibody detecting SV2A in Western Blot, IP. Suitable for Mouse, Rat.

- Over 30 publications
- Trusted since 2006

View Alternative Names

Sv2, Sv2a, Synaptic vesicle glycoprotein 2A, Synaptic vesicle protein 2, Synaptic vesicle protein 2A

2 Images

Supplier Data

Western blot - Anti-SV2A antibody (AB32942)

Blocking buffer : 2% BSA.

All lanes:

Western blot - Anti-SV2A antibody (ab32942) at 1 µg/mL

Lane 1:

Mouse brain at 20 µg

Lane 2:

Rat brain at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 83 kDa

Observed band size: 82 kDa

false

Exposure time: 1min

Unknown

Immunoprecipitation - Anti-SV2A antibody (AB32942)

SV2A was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to SV2A and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70^oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32942.
Secondary : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band : 82kDa : SV2A

All lanes:

Immunoprecipitation - Anti-SV2A antibody (ab32942)

Predicted band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-SV2A antibody (ab32942) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Mouse, Rat samples.

What is the molecular weight of SV2A?
Anti-SV2A (ab32942) specifically detects a band for SV2A (UniProt: Q7L0J3) at a molecular weight of 83kDa.

Trusted by the scientific community
Anti-SV2A (ab32942) was first used in a scientific publication in 2006 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-SV2A (ab32942) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles.. (Microbial infection) Receptor for C.botulinum neurotoxin type A (BoNT/A, botA); the toxin binds via extracellular loop 4 (PubMed : 16543415). Restores uptake of BoNT/A in mouse cells that are deleted for SV2 receptor (PubMed : 16543415, PubMed : 18815274). Glycosylation of Asn-573 is not essential for receptor activity, but enhances uptake (PubMed : 18815274, PubMed : 19650874). Also serves as a receptor for the closely related C.botulinum neurotoxin type A2; glycosylation is not essential but enhances the interaction (PubMed : 29649119).. Possible receptor for C.botulinum neurotoxin type D (BoNT/D, botD); BoNT/D does not bind to extracellular loop 4 as do BoNT/A and BoNT/E, nor to loop 1 or loop 3 (PubMed : 21483489). Another group does not find a convincing interaction with SV2 (PubMed : 21632541).. (Microbial infection) Receptor for C.botulinum neurotoxin type E (BoNT/E); the toxin probably binds via extracellular loop 4 and requires glycosylation of Asn-573 (PubMed : 18815274, PubMed : 19650874). Restores uptake of BoNT/E in mouse cells that are deleted for SV2 receptor (PubMed : 18815274).. (Microbial infection) Receptor for C.botulinum neurotoxin type F (BoNT/F) (PubMed : 19476346, PubMed : 19650874). Binding requires glycosylation of Asn-573 (PubMed : 19476346).

See full target information Sv2a

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Publications (45)

Recent publications for all applications. Explore the full list and refine your search

Journal of neurochemistry 169:e70134 PubMed40542543

2025

Increased Activity-Dependent Bulk Endocytosis in Huntington's Disease Results From Huntingtin Haploinsufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Han C G Tan,Robyn L McAdam,Andrew Morton,Michael A Cousin,Karen J Smillie

Nature communications 16:1579 PubMed39939319

2025

Dense, continuous membrane labeling and expansion microscopy visualization of ultrastructure in tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Tay Won Shin,Hao Wang,Chi Zhang,Bobae An,Yangning Lu,Elizabeth Zhang,Xiaotang Lu,Emmanouil D Karagiannis,Jeong Seuk Kang,Amauche Emenari,Panagiotis Symvoulidis,Shoh Asano,Leanne Lin,Emma K Costa,Adam H Marblestone,Narayanan Kasthuri,Li-Huei Tsai,Edward S Boyden

Neuron 111:4006-4023.e10 PubMed38128479

2023

Serine-129 phosphorylation of α-synuclein is an activity-dependent trigger for physiologic protein-protein interactions and synaptic function.

Applications

Unspecified application

Species

Unspecified reactive species

Leonardo A Parra-Rivas,Kayalvizhi Madhivanan,Brent D Aulston,Lina Wang,Dube Dheeraj Prakashchand,Nicholas P Boyer,Veronica M Saia-Cereda,Kristen Branes-Guerrero,Donald P Pizzo,Pritha Bagchi,V S Sundar,Yong Tang,Utpal Das,David A Scott,Padmini Rangamani,Yuki Ogawa, Subhojit Roy

Nature communications 14:5285 PubMed37648685

2023

Reversal of cell, circuit and seizure phenotypes in a mouse model of DNM1 epileptic encephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine Bonnycastle,Katharine L Dobson,Eva-Maria Blumrich,Akshada Gajbhiye,Elizabeth C Davenport,Marie Pronot,Moritz Steinruecke,Matthias Trost,Alfredo Gonzalez-Sulser,Michael A Cousin

NeuroImage. Clinical 39:103484 PubMed37541098

2023

Assessment of synaptic loss in mouse models of β-amyloid and tau pathology using [F]UCB-H PET imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Letizia Vogler,Anna Ballweg,Bernd Bohr,Nils Briel,Karin Wind,Melissa Antons,Lea H Kunze,Johannes Gnörich,Simon Lindner,Franz-Josef Gildehaus,Karlheinz Baumann,Peter Bartenstein,Guido Boening,Sibylle I Ziegler,Johannes Levin,Andreas Zwergal,Günter U Höglinger,Jochen Herms,Matthias Brendel

Cell reports 42:112633 PubMed37314927

2023

Phosphatidylinositol 4-kinase IIα is a glycogen synthase kinase 3-regulated interaction hub for activity-dependent bulk endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Eva-Maria Blumrich,Jessica C Nicholson-Fish,Marie Pronot,Elizabeth C Davenport,Dominic Kurian,Adam Cole,Karen J Smillie,Michael A Cousin

The Journal of neuroscience : the official journal of the Society for Neuroscience 43:2002-2020 PubMed36759195

2023

Epilepsy-Related CDKL5 Deficiency Slows Synaptic Vesicle Endocytosis in Central Nerve Terminals.

Applications

Unspecified application

Species

Unspecified reactive species

Christiana Kontaxi,Daniela Ivanova,Elizabeth C Davenport,Peter C Kind,Michael A Cousin

Proceedings of the National Academy of Sciences of the United States of America 119:e2202536119 PubMed36161898

2022

Dissection of the long-range projections of specific neurons at the synaptic level in the whole mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaojiao Tian,Miao Ren,Peilin Zhao,Shukang Luo,Yingying Chen,Xiaofeng Xu,Tao Jiang,Qingtao Sun,Anan Li,Hui Gong,Xiangning Li,Qingming Luo

Nature communications 13:3690 PubMed35760976

2022

The 22q11.2 region regulates presynaptic gene-products linked to schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Ralda Nehme,Olli Pietiläinen,Mykyta Artomov,Matthew Tegtmeyer,Vera Valakh,Leevi Lehtonen,Christina Bell,Tarjinder Singh,Aditi Trehan,John Sherwood,Danielle Manning,Emily Peirent,Rhea Malik,Ellen J Guss,Derek Hawes,Amanda Beccard,Anne M Bara,Dane Z Hazelbaker,Emanuela Zuccaro,Giulio Genovese,Alexander A Loboda,Anna Neumann,Christina Lilliehook,Outi Kuismin,Eija Hamalainen,Mitja Kurki,Christina M Hultman,Anna K Kähler,Joao A Paulo,Andrea Ganna,Jon Madison,Bruce Cohen,Donna McPhie,Rolf Adolfsson,Roy Perlis,Ricardo Dolmetsch,Samouil Farhi,Steven McCarroll,Steven Hyman,Ben Neale,Lindy E Barrett,Wade Harper,Aarno Palotie,Mark Daly,Kevin Eggan

Cell reports 39:110883 PubMed35649351

2022

Sustained Trem2 stabilization accelerates microglia heterogeneity and Aβ pathology in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Rahul Dhandapani,Marilisa Neri,Mario Bernhard,Irena Brzak,Tatjana Schweizer,Stefan Rudin,Stefanie Joller,Ramon Berth,Jasmin Kernen,Anna Neuhaus,Annick Waldt,Rachel Cuttat,Ulrike Naumann,Caroline Gubser Keller,Guglielmo Roma,Dominik Feuerbach,Derya R Shimshek,Ulf Neumann,Fabrizio Gasparini,Ivan Galimberti

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SV2A antibody

Western blot - Anti-SV2A antibody (AB32942)

Immunoprecipitation - Anti-SV2A antibody (AB32942)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (45)

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