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AB324579

Anti-SV2A antibody [EPR23500-201] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal SV2A antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse samples.

View Alternative Names

Kiaa0736, Sv2, Sv2a, Synaptic vesicle glycoprotein 2A, Synaptic vesicle protein 2, Synaptic vesicle protein 2A, Calcium regulator SV2A

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Sandwich ELISA - Anti-SV2A antibody [EPR23500-201] - BSA and Azide free (Detector) (AB324579)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-SV2A antibody [EPR23500-201] - BSA and Azide free (Detector) (AB324579)

Interpolated concentration of native SV2A was measured at different sample concentrations. Undiluted samples are as follows : human brain tissue extract (50 µg/mL), mouse brain tissue extract (50 µg/mL) and SH-SY5Y cell extract (1000 µg/mL). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-SV2A antibody [EPR23500-201] - BSA and Azide free (Detector) (AB324579)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-SV2A antibody [EPR23500-201] - BSA and Azide free (Detector) (AB324579)

Sandwich ELISA using Human SV2A protein standard curve with the capture antibody (ab324578) at 2 µg/mL and detector antibody (ab324579) at 0.5 µg/mL. Background-subtracted absorbance data values (mean +/- standard deviation are shown.)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23500-201

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab324579 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Mouse SV2A. The recommended pair for sandwich ELISA is:

Capture: ab324578, Human/Mouse SV2A Capture Antibody (unconjugated)

Detector: ab324579, Human/Mouse SV2A Detector Antibody (unconjugated)

The reference range value is 19.5-1250 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles.. (Microbial infection) Receptor for C.botulinum neurotoxin type A (BoNT/A, botA); the toxin probably binds via extracellular loop 4 (PubMed : 16543415).. (Microbial infection) Possible receptor for C.botulinum neurotoxin type D (BoNT/D, botD); BoNT/D does not bind to extracellular loop 4 as do BoNT/A and BoNT/E (PubMed : 21483489).. (Microbial infection) Receptor for C.botulinum neurotoxin type E (BoNT/E); the toxin probably binds via extracellular loop 4 (PubMed : 18815274). It probably requires glycosylation of Asn-573 (PubMed : 18815274).
See full target information Sv2a
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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