AB234426

Anti-SV40 T-antigen antibody [EPR22694-148]

RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal LT antibody. Suitable for WB, ICC/IF, IP, Flow Cyt (Intra) and reacts with Simian Virus 40 samples. Cited in 6 publications.

View Alternative Names

Large T antigen, LT, LT-AG, DNA 3'-5' helicase large T antigen, LT AG, Large T antigen

4 Images

Flow Cytometry (Intracellular) - Anti-SV40 T-antigen antibody [EPR22694-148] (AB234426)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-SV40 T-antigen antibody [EPR22694-148] (AB234426)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293 (human embryonic kidney epithelial cell, Left) / 293T (SV40 transfected human embryonic kidney epithelial cell, Right) cells labeling SV40 T-antigen with ab234426 at 1/500 dilution (red), compared with aRabbit monoclonal IgG isotype control (ab172730,Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/2000 dilution.

Negative control : HEK-293.

Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [EPR22694-148] (AB234426)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [EPR22694-148] (AB234426)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labeling SV40 T-antigen with ab234426 at 1/50 dilution, followed by an AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) (Green) at 1/1000 dilution. Confocal image showing nuclear and weak cytoplasmic staining in HEK-293T cells. The nuclear counterstain is DAPI (Blue). Tubulin was stained using an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (Red) at 1/200 dilution.

Negative control : HEK-293(PMID : 15175262).

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Immunoprecipitation - Anti-SV40 T-antigen antibody [EPR22694-148] (AB234426)

SV40 T-antigen was immunoprecipitated from 0.35 mg HEK-293T (SV40 transfected human embryonic kidney epithelial cell) whole cell lysate with ab234426 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab234426 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

Lane 1 : HEK-293T (SV40 transfected) whole cell lysate 10 μg (input).
Lane 2 : ab234426 IP in HEK-293T (SV40 transfected) whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab234426 in HEK-293T whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426)

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Western blot - Anti-SV40 T-antigen antibody [EPR22694-148] (AB234426)

Blocking buffer and concentration : 5% NFDM/TBST

Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426) at 1/5000 dilution

Lane 1:

293T (SV40 transfected human embryonic kidney epithelial cell) whole cell lysate prepared in RIPA lysis method at 20 µg

Lane 2:

293T (SV40 transfected human embryonic kidney epithelial cell) whole cell lysate prepared in 1%SDS Hot lysis method at 20 µg

Lane 3:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate prepared in RIPA lysis method at 20 µg

Lane 4:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate prepared in 1%SDS Hot lysis method at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 90 kDa

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Exposure time: 40s

Carrier free

Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22694-148

Isotype

IgG

Carrier free

Reacts with

Simian Virus 40

Applications

WB, Flow Cyt (Intra), ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Simian Virus 40": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. Inhibits host TP53 binding to DNA, abrogating the ability of TP53 to stimulate gene expression. Plays the role of a TFIID-associated factor (TAF) in transcription initiation for all three RNA polymerases, by stabilizing the TBP-TFIIA complex on promoters. Initiates viral DNA replication and unwinding via interactions with the viral origin of replication. Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Has processive 3'-5' DNA helicase activity which requires a short 3' single-stranded region and ATP; other (d)NTPs can partially replace ATP (PubMed : 2826443, PubMed : 2826446). Activates the transcription of viral late mRNA, through host TBP and TFIIA stabilization. Interferes with histone deacetylation mediated by HDAC1, leading to activation of transcription. May inactivate the growth-suppressing properties of the E3 ubiquitin ligase CUL7.. Isoform 17kT antigen targets host RBL2 for degradation and promotes cell proliferation. Transactivates host cyclin A promoter through its J domain.. Unwinds G4 DNA (planar arrays of 4 guanine bases stabilized by hydrogen bonds, parallel and antiparallel arrays were tested); unwinding occurs in the 3'-5' direction, requires a 3' single-stranded end and hydrolyzable ATP (PubMed : 9016557).

See full target information Large T antigen

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadx6587 PubMed40815641

2025

Targeting lipid scrambling potentiates ferroptosis and triggers tumor immune rejection.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyun Yang,Ze Yu,Jieming Ping,Yuanchun Duan,Jianlong Tang,Weixiang Liu,Qing He,Yongfeng Lai,Sin Man Lam,Hesen Tang,Zhengjie Liu,Weimin Wang,Min Zhu,Wei Hu,Yunyun Han,Guanghou Shui,Jiqing Hao,Zheng Liu,Ning Wu

Scientific reports 14:11591 PubMed38773220

2024

A simple protocol to establish a conditionally immortalized mouse podocyte cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Yujiao Huang,Jie Geng,Mengdan Wang,Wenbin Liu,Haikun Hu,Wei Shi,Mei Li,Guiyang Huo,Guangrui Huang,Anlong Xu

Cells 13: PubMed38667318

2024

Establishment and Characterization of SV40 T-Antigen Immortalized Porcine Muscle Satellite Cell.

Applications

Unspecified application

Species

Unspecified reactive species

Mengru Ni,Jingqing He,Tao Li,Gan Zhao,Zhengyu Ji,Fada Ren,Jianxin Leng,Mengyan Wu,Ruihua Huang,Pinghua Li,Liming Hou

Microbiology spectrum 11:e0275123 PubMed37815349

2023

Comprehensive proteomic analysis of JC polyomavirus-infected human astrocytes and their extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Larise Oberholster,Amandine Mathias,Sylvain Perriot,Emma Blaser,Mathieu Canales,Samuel Jones,Lucas Culebras,Marie Gimenez,G Campbell Kaynor,Alexey Sapozhnik,Kevin Richetin,Susan Goelz,Renaud Du Pasquier

iScience 26:105995 PubMed36687314

2023

SARS-CoV-2 N protein mediates intercellular nucleic acid dispersion, a feature reduced in Omicron.

Applications

Unspecified application

Species

Unspecified reactive species

Jung-Lin Wu,I-I Kuan,Jing-You Guo,Wei-Chia Hsu,Wei-Chun Tang,Hsin-Ju Chan,Yu-Ju Chen,Bi-Chang Chen,Han-Chung Wu,James C Liao

Scientific reports 12:15001 PubMed36056070

2022

Adult fibroblasts use aggresomes only in distinct cell-states.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher S Morrow,Zachary P Arndt,Payton C Klosa,Bo Peng,Eden Y Zewdie,Bérénice A Benayoun,Darcie L Moore

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SV40 T-antigen antibody [EPR22694-148]

Flow Cytometry (Intracellular) - Anti-SV40 T-antigen antibody [EPR22694-148] (AB234426)

Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [EPR22694-148] (AB234426)

Immunoprecipitation - Anti-SV40 T-antigen antibody [EPR22694-148] (AB234426)

Western blot - Anti-SV40 T-antigen antibody [EPR22694-148] (AB234426)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise