Rabbit Recombinant Monoclonal LT antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Simian Virus 40 samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (AB255286), expandable thumbnail](https://content.abcam.com/products/images/sv40-t-antigen-antibody-epr22694-148-bsa-and-azide-free-ab255286--immunocytochemistry-immunofluorescence-img77116.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (AB255286)")
![Immunoprecipitation - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (AB255286), expandable thumbnail](https://content.abcam.com/products/images/sv40-t-antigen-antibody-epr22694-148-bsa-and-azide-free-ab255286--immunoprecipitation-img3311.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (AB255286)")
![Flow Cytometry (Intracellular) - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (AB255286), expandable thumbnail](https://content.abcam.com/products/images/sv40-t-antigen-antibody-epr22694-148-bsa-and-azide-free-ab255286--flow-cytometry-intracellular-img12959.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (AB255286)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|
| Simian Virus 40 | Tested | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Simian Virus 40 | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Simian Virus 40 | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Simian Virus 40 | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Simian Virus 40 | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. Inhibits host TP53 binding to DNA, abrogating the ability of TP53 to stimulate gene expression. Plays the role of a TFIID-associated factor (TAF) in transcription initiation for all three RNA polymerases, by stabilizing the TBP-TFIIA complex on promoters. Initiates viral DNA replication and unwinding via interactions with the viral origin of replication. Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Has processive 3'-5' DNA helicase activity which requires a short 3' single-stranded region and ATP; other (d)NTPs can partially replace ATP (PubMed:2826443, PubMed:2826446). Activates the transcription of viral late mRNA, through host TBP and TFIIA stabilization. Interferes with histone deacetylation mediated by HDAC1, leading to activation of transcription. May inactivate the growth-suppressing properties of the E3 ubiquitin ligase CUL7. Isoform 17kT antigen targets host RBL2 for degradation and promotes cell proliferation. Transactivates host cyclin A promoter through its J domain. Unwinds G4 DNA (planar arrays of 4 guanine bases stabilized by hydrogen bonds, parallel and antiparallel arrays were tested); unwinding occurs in the 3'-5' direction, requires a 3' single-stranded end and hydrolyzable ATP (PubMed:9016557).
Additional Targets
SV40 T-antigen
Alternative names
Large T antigen, LT, LT-AG, DNA 3'-5' helicase large T antigen
Recommended products
Rabbit Recombinant Monoclonal LT antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Simian Virus 40 samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22694-148
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab255286 is the carrier-free version of Anti-SV40 T-antigen antibody [EPR22694-148] ab234426.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (ab255286), expandable thumbnail](https://content.abcam.com/products/images/sv40-t-antigen-antibody-epr22694-148-bsa-and-azide-free-ab255286--immunocytochemistry-immunofluorescence-img77116.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (ab255286)")
Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (ab255286)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labeling SV40 T-antigen with Anti-SV40 T-antigen antibody [EPR22694-148] ab234426 at 1/50 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (Green) at 1/1000 dilution. Confocal image showing nuclear and weak cytoplasmic staining in 293T cells. The nuclear counterstain is DAPI (Blue). Tubulin was stained using an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)(Red) at 1/200 dilution.
Negative control: HEK-293(PMID: 15175262).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SV40 T-antigen antibody [EPR22694-148] ab234426).
![Immunoprecipitation - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (ab255286), expandable thumbnail](https://content.abcam.com/products/images/sv40-t-antigen-antibody-epr22694-148-bsa-and-azide-free-ab255286--immunoprecipitation-img3311.jpg "Immunoprecipitation - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (ab255286)")
Immunoprecipitation - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (ab255286)
SV40 T-antigen was immunoprecipitated from 0.35 mg HEK-293T (SV40 transfected human embryonic kidney epithelial cell) whole cell lysate with Anti-SV40 T-antigen antibody [EPR22694-148] ab234426 at 1/30 dilution. Western blot was performed on the immunoprecipitate using Anti-SV40 T-antigen antibody [EPR22694-148] ab234426 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: HEK-293T (SV40 transfected) whole cell lysate 10 μg (input).
Lane 2: Anti-SV40 T-antigen antibody [EPR22694-148] ab234426 IP in HEK-293T (SV40 transfected) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-SV40 T-antigen antibody [EPR22694-148] ab234426 in HEK-293T whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SV40 T-antigen antibody [EPR22694-148] ab234426).
All lanes: Immunoprecipitation - Anti-SV40 T-antigen antibody [EPR22694-148] (Anti-SV40 T-antigen antibody [EPR22694-148] ab234426)
![Flow Cytometry (Intracellular) - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (ab255286), expandable thumbnail](https://content.abcam.com/products/images/sv40-t-antigen-antibody-epr22694-148-bsa-and-azide-free-ab255286--flow-cytometry-intracellular-img12959.jpg "Flow Cytometry (Intracellular) - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (ab255286)")
Flow Cytometry (Intracellular) - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (ab255286)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293 (human embryonic kidney epithelial cell, Left) / 293T (SV40 transfected human embryonic kidney epithelial cell, Right) cells labeling SV40 T-antigen with Anti-SV40 T-antigen antibody [EPR22694-148] ab234426 at 1/500 dilution (red), compared with aRabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730,Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/2000 dilution.
Negative control: HEK-293.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SV40 T-antigen antibody [EPR22694-148] ab234426).
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