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AB255286

Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal LT antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Simian Virus 40 samples. Cited in 1 publication.

View Alternative Names

Large T antigen, LT, LT-AG, DNA 3'-5' helicase large T antigen

3 Images
Flow Cytometry (Intracellular) - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (AB255286)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (AB255286)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293 (human embryonic kidney epithelial cell, Left) / 293T (SV40 transfected human embryonic kidney epithelial cell, Right) cells labeling SV40 T-antigen with ab234426 at 1/500 dilution (red), compared with aRabbit monoclonal IgG isotype control (ab172730,Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution.

Negative control : HEK-293.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234426).

Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (AB255286)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (AB255286)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labeling SV40 T-antigen with ab234426 at 1/50 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) (Green) at 1/1000 dilution. Confocal image showing nuclear and weak cytoplasmic staining in 293T cells. The nuclear counterstain is DAPI (Blue). Tubulin was stained using an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Red) at 1/200 dilution.

Negative control : HEK-293(PMID : 15175262).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234426).

Immunoprecipitation - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (AB255286)
  • IP

Unknown

Immunoprecipitation - Anti-SV40 T-antigen antibody [EPR22694-148] - BSA and Azide free (AB255286)

SV40 T-antigen was immunoprecipitated from 0.35 mg HEK-293T (SV40 transfected human embryonic kidney epithelial cell) whole cell lysate with ab234426 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab234426 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

Lane 1 : HEK-293T (SV40 transfected) whole cell lysate 10 μg (input).
Lane 2 : ab234426 IP in HEK-293T (SV40 transfected) whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab234426 in HEK-293T whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234426).

All lanes:

Immunoprecipitation - Anti-SV40 T-antigen antibody [EPR22694-148] (<a href='/en-us/products/primary-antibodies/sv40-t-antigen-antibody-epr22694-148-ab234426'>ab234426</a>)

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22694-148

Isotype

IgG

Carrier free

Yes

Reacts with

Simian Virus 40

Applications

WB, ICC/IF, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Simian Virus 40": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }
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Product details

ab255286 is the carrier-free version of ab234426.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. Inhibits host TP53 binding to DNA, abrogating the ability of TP53 to stimulate gene expression. Plays the role of a TFIID-associated factor (TAF) in transcription initiation for all three RNA polymerases, by stabilizing the TBP-TFIIA complex on promoters. Initiates viral DNA replication and unwinding via interactions with the viral origin of replication. Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Has processive 3'-5' DNA helicase activity which requires a short 3' single-stranded region and ATP; other (d)NTPs can partially replace ATP (PubMed : 2826443, PubMed : 2826446). Activates the transcription of viral late mRNA, through host TBP and TFIIA stabilization. Interferes with histone deacetylation mediated by HDAC1, leading to activation of transcription. May inactivate the growth-suppressing properties of the E3 ubiquitin ligase CUL7.. Isoform 17kT antigen targets host RBL2 for degradation and promotes cell proliferation. Transactivates host cyclin A promoter through its J domain.. Unwinds G4 DNA (planar arrays of 4 guanine bases stabilized by hydrogen bonds, parallel and antiparallel arrays were tested); unwinding occurs in the 3'-5' direction, requires a 3' single-stranded end and hydrolyzable ATP (PubMed : 9016557).
See full target information Large T antigen
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:514 PubMed40155473

2025

Defining gastric cancer ecology: the crucial roles of TREM2 macrophages and fibroblasts in tumor microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Cao,Dianshui Sun,Can Tu,Jihua Wang,Runjia Fu,Rumei Gong,Yueying Xiao,Qin Liu,Xiaomei Li
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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