Anti-SVH antibody [EPR13763] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SVH antibody [EPR13763] - BSA and Azide free (AB250967)

This data was developed using ab188573, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed A431cells labeling SVH with ab188573 at 1/50 followed by Goat anti rabbit IgG (Alexa Fluor®488) and DAPI staining.

• IP

Supplier Data

Immunoprecipitation - Anti-SVH antibody [EPR13763] - BSA and Azide free (AB250967)

This data was developed using ab188573, the same antibody clone in a different buffer formulation.

Western blot analysis of SVH in immunoprecipitation pellets from U87-MG lysate. ab188573 used at 1/50 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-SVH antibody [EPR13763] - C-terminal (<a href='/en-us/products/primary-antibodies/svh-antibody-epr13763-c-terminal-ab188573'>ab188573</a>) at 1/1000 dilution

Lane 1:

U87-MG

Lane 2:

PBS instead of U87-MG lysates

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

Predicted band size: 38 kDa

Observed band size: 27 kDa,34 kDa

false

• WB

Supplier Data

Western blot - Anti-SVH antibody [EPR13763] - BSA and Azide free (AB250967)

This data was developed using ab188573, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SVH antibody [EPR13763] - C-terminal (<a href='/en-us/products/primary-antibodies/svh-antibody-epr13763-c-terminal-ab188573'>ab188573</a>) at 1/1000 dilution

Lane 1:

SKBR-3 cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

U87-MG cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

• WB

Supplier Data

Western blot - Anti-SVH antibody [EPR13763] - BSA and Azide free (AB250967)

This data was developed using ab188573, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SVH antibody [EPR13763] - C-terminal (<a href='/en-us/products/primary-antibodies/svh-antibody-epr13763-c-terminal-ab188573'>ab188573</a>) at 1/1000 dilution

Lane 1:

C6 lysate at 10 µg

Lane 2:

RAW264.7 lysate at 10 µg

Lane 3:

PC-12 lysate at 10 µg

Lane 4:

NIH3T3 lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false