Rabbit Recombinant Monoclonal SVH antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Rat, Mouse samples.
Images
![Western blot - Anti-SVH antibody [EPR13763] - BSA and Azide free (AB250967), expandable thumbnail](https://content.abcam.com/products/images/svh-antibody-epr13763-bsa-and-azide-free-ab250967--western-blot-img104464.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SVH antibody [EPR13763] - BSA and Azide free (AB250967)")
![Immunocytochemistry/ Immunofluorescence - Anti-SVH antibody [EPR13763] - BSA and Azide free (AB250967), expandable thumbnail](https://content.abcam.com/products/images/svh-antibody-epr13763-bsa-and-azide-free-ab250967--immunocytochemistry-immunofluorescence-img100480.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SVH antibody [EPR13763] - BSA and Azide free (AB250967)")
![Immunoprecipitation - Anti-SVH antibody [EPR13763] - BSA and Azide free (AB250967), expandable thumbnail](https://content.abcam.com/products/images/svh-antibody-epr13763-bsa-and-azide-free-ab250967--immunoprecipitation-img55652.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SVH antibody [EPR13763] - BSA and Azide free (AB250967)")
![Western blot - Anti-SVH antibody [EPR13763] - BSA and Azide free (AB250967), expandable thumbnail](https://content.abcam.com/products/images/svh-antibody-epr13763-bsa-and-azide-free-ab250967--western-blot-img127358.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SVH antibody [EPR13763] - BSA and Azide free (AB250967)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
| Rat | Expected | Expected | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
Target data
Function
May play a role in cell survival and cell growth. May suppress the transcriptional activity of p53/TP53.
Alternative names
SVH, PSEC0198, ARMC10, Armadillo repeat-containing protein 10, Splicing variant involved in hepatocarcinogenesis protein
Recommended products
Rabbit Recombinant Monoclonal SVH antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13763
- Specificity
Based on the sequence analysis, this antibody recognizes 5 isoforms with the predicted MWs of 38KDa, 34KDa, 31KDa, 27KDa and 31KDa, respectively.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250967 is the carrier-free version of Anti-SVH antibody [EPR13763] - C-terminal ab188573.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-SVH antibody [EPR13763] - BSA and Azide free (ab250967), expandable thumbnail](https://content.abcam.com/products/images/svh-antibody-epr13763-bsa-and-azide-free-ab250967--western-blot-img104464.jpg "Western blot - Anti-SVH antibody [EPR13763] - BSA and Azide free (ab250967)")
Western blot - Anti-SVH antibody [EPR13763] - BSA and Azide free (ab250967)
This data was developed using Anti-SVH antibody [EPR13763] - C-terminal ab188573, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SVH antibody [EPR13763] - C-terminal (Anti-SVH antibody [EPR13763] - C-terminal ab188573) at 1/1000 dilution
Lane 1: SKBR-3 cell lysate at 20 µg
Lane 2: A431 cell lysate at 20 µg
Lane 3: HepG2 cell lysate at 20 µg
Lane 4: U87-MG cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-SVH antibody [EPR13763] - BSA and Azide free (ab250967), expandable thumbnail](https://content.abcam.com/products/images/svh-antibody-epr13763-bsa-and-azide-free-ab250967--immunocytochemistry-immunofluorescence-img100480.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SVH antibody [EPR13763] - BSA and Azide free (ab250967)")
Immunocytochemistry/ Immunofluorescence - Anti-SVH antibody [EPR13763] - BSA and Azide free (ab250967)
This data was developed using Anti-SVH antibody [EPR13763] - C-terminal ab188573, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed A431cells labeling SVH with Anti-SVH antibody [EPR13763] - C-terminal ab188573 at 1/50 followed by Goat anti rabbit IgG (Alexa Fluor®488) and DAPI staining.
![Immunoprecipitation - Anti-SVH antibody [EPR13763] - BSA and Azide free (ab250967), expandable thumbnail](https://content.abcam.com/products/images/svh-antibody-epr13763-bsa-and-azide-free-ab250967--immunoprecipitation-img55652.jpg "Immunoprecipitation - Anti-SVH antibody [EPR13763] - BSA and Azide free (ab250967)")
Immunoprecipitation - Anti-SVH antibody [EPR13763] - BSA and Azide free (ab250967)
This data was developed using Anti-SVH antibody [EPR13763] - C-terminal ab188573, the same antibody clone in a different buffer formulation.
Western blot analysis of SVH in immunoprecipitation pellets from U87-MG lysate. Anti-SVH antibody [EPR13763] - C-terminal ab188573 used at 1/50 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1500 dilution.
All lanes: Immunoprecipitation - Anti-SVH antibody [EPR13763] - C-terminal (Anti-SVH antibody [EPR13763] - C-terminal ab188573) at 1/1000 dilution
Lane 1: U87-MG
Lane 2: PBS instead of U87-MG lysates
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
Predicted band size: 38 kDa
Observed band size: 27 kDa, 34 kDa
![Western blot - Anti-SVH antibody [EPR13763] - BSA and Azide free (ab250967), expandable thumbnail](https://content.abcam.com/products/images/svh-antibody-epr13763-bsa-and-azide-free-ab250967--western-blot-img127358.jpg "Western blot - Anti-SVH antibody [EPR13763] - BSA and Azide free (ab250967)")
Western blot - Anti-SVH antibody [EPR13763] - BSA and Azide free (ab250967)
This data was developed using Anti-SVH antibody [EPR13763] - C-terminal ab188573, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SVH antibody [EPR13763] - C-terminal (Anti-SVH antibody [EPR13763] - C-terminal ab188573) at 1/1000 dilution
Lane 1: C6 lysate at 10 µg
Lane 2: RAW264.7 lysate at 10 µg
Lane 3: PC-12 lysate at 10 µg
Lane 4: NIH3T3 lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa
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