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AB228846

Anti-SWAP70 antibody - C-terminal

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(3 Publications)

Rabbit Polyclonal SWAP70 antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SWAP70.

View Alternative Names

KIAA0640, HSPC321, SWAP70, Switch-associated protein 70, SWAP-70

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SWAP70 antibody - C-terminal (AB228846)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SWAP70 antibody - C-terminal (AB228846)

SK-N-SH (human neuroblastoma cell line) cells stained for SWAP70 (green) using ab228846 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SWAP70 antibody - C-terminal (AB228846)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SWAP70 antibody - C-terminal (AB228846)

Paraffin-embedded mouse spleen tissue stained for SWAP70 with ab228846 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-SWAP70 antibody - C-terminal (AB228846)
  • WB

Supplier Data

Western blot - Anti-SWAP70 antibody - C-terminal (AB228846)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-SWAP70 antibody - C-terminal (ab228846) at 1/10000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extracts at 30 µg

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell extracts at 30 µg

Lane 3:

NCI-H1299 (human lung carcinoma cell line) whole cell extracts at 30 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg

Lane 5:

A431 (human epidermoid carcinoma cell line) whole cell extracts at 30 µg

Lane 6:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts at 30 µg

Lane 7:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts at 30 µg

Predicted band size: 69 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SWAP70. The exact immunogen used to generate this antibody is proprietary information.

Q9UH65

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion (By similarity).
See full target information SWAP70
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:977251 PubMed36727078

2023

Integrated analysis of genome-wide DNA methylation and cancer-associated fibroblasts identified prognostic biomarkers and immune checkpoint blockade in lower grade gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawei Dong,Fang Wang,Xin Gao,Hongtao Zhao,Jiheng Zhang,Nan Wang,Zhihui Liu,Xiuwei Yan,Jiaqi Jin,Yixu Ba,Shuai Ma,Jianyang Du,Hang Ji,Shaoshan Hu

The Journal of cell biology 220: PubMed34287648

2021

The glycoprotein GP130 governs the surface presentation of the G protein-coupled receptor APLNR.

Applications

Unspecified application

Species

Unspecified reactive species

Kilian Trillet,Kathryn A Jacobs,Gwennan André-Grégoire,An Thys,Clément Maghe,Jonathan Cruard,Stéphane Minvielle,Sara Gonzalez Diest,Guillaume Montagnac,Nicolas Bidère,Julie Gavard

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 23:449-463 PubMed31673844

2019

LBX2-AS1/miR-219a-2-3p/FUS/LBX2 positive feedback loop contributes to the proliferation of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Yang,Xinhua Dong,Minglong Pu,Hongwei Yang,Weilong Chang,Feihong Ji,Tao Liu,Chongqing Wei,Xiefu Zhang,Xinguang Qiu
View all publications
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