Rabbit Polyclonal SWAP70 antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SWAP70.
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Publications
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- Frontiers in oncology 12:9772512023Integrated analysis of genome-wide DNA methylation and cancer-associated fibroblasts identified prognostic biomarkers and immune checkpoint blockade in lower grade gliomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiawei Dong,Fang Wang,Xin Gao,Hongtao Zhao,Jiheng Zhang,Nan Wang,Zhihui Liu,Xiuwei Yan,Jiaqi Jin,Yixu Ba,Shuai Ma,Jianyang Du,Hang Ji,Shaoshan HuPubMed 36727078
- The Journal of cell biology 220:2021The glycoprotein GP130 governs the surface presentation of the G protein-coupled receptor APLNR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKilian Trillet,Kathryn A Jacobs,Gwennan André-Grégoire,An Thys,Clément Maghe,Jonathan Cruard,Stéphane Minvielle,Sara Gonzalez Diest,Guillaume Montagnac,Nicolas Bidère,Julie GavardPubMed 34287648
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SWAP70. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UH65
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000.00000 - 1/20000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Rhesus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Rhesus monkey | Dilution info - | Notes - |
Target data
Function
Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion (By similarity).
Alternative names
KIAA0640, HSPC321, SWAP70, Switch-associated protein 70, SWAP-70
Recommended products
Rabbit Polyclonal SWAP70 antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SWAP70.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SWAP70. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UH65
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-SWAP70 antibody - C-terminal (ab228846)
7.5% SDS-PAGE
All lanes: Western blot - Anti-SWAP70 antibody - C-terminal (ab228846) at 1/10000 dilution
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extracts at 30 µg
Lane 2: Raji (human Burkitt's lymphoma cell line) whole cell extracts at 30 µg
Lane 3: NCI-H1299 (human lung carcinoma cell line) whole cell extracts at 30 µg
Lane 4: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg
Lane 5: A431 (human epidermoid carcinoma cell line) whole cell extracts at 30 µg
Lane 6: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts at 30 µg
Lane 7: HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts at 30 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SWAP70 antibody - C-terminal (ab228846)
SK-N-SH (human neuroblastoma cell line) cells stained for SWAP70 (green) using ab228846 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SWAP70 antibody - C-terminal (ab228846)
Paraffin-embedded mouse spleen tissue stained for SWAP70 with ab228846 at 1/500 dilution in immunohistochemical analysis.
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