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AB220240

Anti-Swd2 antibody

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(2 Publications)

Rabbit Polyclonal Swd2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WDR82 aa 200 to C-terminus.

View Alternative Names

SWD2, TMEM113, WDR82A, UNQ9342/PRO34047, WDR82, WD repeat-containing protein 82

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Swd2 antibody (AB220240)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Swd2 antibody (AB220240)

Immunofluorescent analysis of U-251 MG cells (PFA-fixed/Triton X-100 permeabilized) labeling Swd2 with ab220240 at 4μg/ml (green).

Western blot - Anti-Swd2 antibody (AB220240)
  • WB

Supplier Data

Western blot - Anti-Swd2 antibody (AB220240)

All lanes:

Western blot - Anti-Swd2 antibody (ab220240) at 1/100 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human WDR82 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6UXN9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Swd2 also known as Cwc21 or Swd2p is a protein that functions as a regulatory component in RNA splicing and transcription. This protein has a mass of approximately 47 kDa and is primarily expressed in the nucleus of eukaryotic cells. Swd2 associates with the spliceosome and transcription-related complexes playing a role in coordinating transcription and processing of RNA. Understanding its precise mechanical role is key to appreciate its broader biological implications.
Biological function summary

Swd2 operates as part of the COMPASS complex a multiprotein assembly involved in histone modification. This complex methylates histone H3 at lysine 4 (H3K4) influencing chromatin structure and gene expression regulation. Swd2 contributes to the function of the spliceosome by interacting with core splicing machinery components aiding the removal of introns from pre-mRNA. Its activity affects gene expression patterns linking transcription processes with RNA maturation in the cell.

Pathways

Swd2 is integral within two major biological pathways: RNA splicing and chromatin modification. Through RNA splicing it assists with the precise excision of introns interacting with proteins like Prp19. In the chromatin modification pathway it acts as part of the COMPASS complex which involves Set1 in the methylation of H3K4. This methylation is critical for chromatin remodeling therefore impacting transcription regulation and gene silencing.

Researchers have noted associations between Swd2's function and certain cancers and neurodegenerative disorders. Aberrations in RNA splicing regulation where Swd2 is involved may contribute to oncogenesis. Altered Swd2 expression or function can disrupt the careful balance required for normal cell proliferation. Increased aberrant splicing or disrupted trimethylation at H3K4 potentially linked to Set1 may lead to pathways involved in these disorders. Understanding Swd2's pathways could be important for therapeutic exploration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory component of the SET1/COMPASS complex implicated in the tethering of this complex to transcriptional start sites of active genes (PubMed : 17998332, PubMed : 18838538, PubMed : 20516061). Facilitates histone H3 'Lys-4' methylation (H3K4me) via recruitment of the SETD1A or SETD1B to the 'Ser-5' phosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A) (PubMed : 17998332, PubMed : 18838538). Component of the PNUTS-PP1 protein phosphatase complex, a protein phosphatase 1 (PP1) complex that promotes RNA polymerase II transcription pause-release, allowing transcription elongation (PubMed : 39603240, PubMed : 39603239). PNUTS-PP1 also plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase (PubMed : 20516061). Together with ZC3H4, but independently of the SET1 complex, part of a transcription termination checkpoint that promotes transcription termination of long non-coding RNAs (lncRNAs) (PubMed : 33767452, PubMed : 33913806). The transcription termination checkpoint is activated by the inefficiently spliced first exon of lncRNAs and promotes transcription termination of lncRNAs and their subsequent degradation by the exosome (PubMed : 33767452).
See full target information WDR82
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 25:105620 PubMed36465115

2022

TWIK-related acid-sensitive K channel 2 promotes renal fibrosis by inducing cell-cycle arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhang,Jing Chen,Yufei Lu,Yan Yang,Weize Chen,Bo Shen,Jiachang Hu,Ping Jia,Sujuan Xu,Yiqin Shi,Yichun Ning,Jialin Wang,Yi Fang,Shuan Zhao,Yang Li,Yan Dai,Xiaoyan Zhang,Meng Xiang,Yang Tian,Zhichao Liu,Nana Song,Xiaoqiang Ding

Virulence 11:607-635 PubMed32420802

2020

TMT-based quantitative proteomics analysis reveals the attenuated replication mechanism of Newcastle disease virus caused by nuclear localization signal mutation in viral matrix protein.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqiang Duan,Chao Yuan,Yifan Han,Lei Zhou,Jiafu Zhao,Yong Ruan,Jiaqi Chen,Mengmeng Ni,Xinqin Ji
View all publications
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