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Rabbit Recombinant Monoclonal Swd2 antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Mouse, Human, Rat samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Tested
Tested
Tested
Tested
Rat
Expected
Expected
Expected
Tested
Tested

Tested
Tested

Species

Mouse, Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse, Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse, Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Target data

Function

Regulatory component of the SET1 complex implicated in the tethering of this complex to transcriptional start sites of active genes. Facilitates histone H3 'Lys-4' methylation via recruitment of the SETD1A or SETD1B to the 'Ser-5' phosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.

Alternative names

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Rabbit Recombinant Monoclonal Swd2 antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Mouse, Human, Rat samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Carrier free

Yes

Clone number

EPR27034-63

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Western blot - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555), expandable thumbnail

    Western blot - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555)

    This data was developed using ab307554, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 103 seconds

    All lanes: Western blot - Anti-Swd2 antibody [EPR27034-63] (AB307554) at 1/1000 dilution

    Lane 1: Human colon tissue lysate at 10 µg

    Lane 2: Mouse colon tissue lysate at 10 µg

    Lane 3: Rat colon tissue lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Observed band size: 35 kDa

    Exposure time: 103s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 103 seconds

  • Western blot - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555), expandable thumbnail

    Western blot - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555)

    This data was developed using ab307554, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 27 seconds

    All lanes: Western blot - Anti-Swd2 antibody [EPR27034-63] (AB307554) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeti Swd2, whole cell lysate at 20 µg

    Lane 3: HeLa whole cell lysate at 20 µg

    Lane 4: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Observed band size: 35 kDa

    Exposure time: 27s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 27 seconds

  • Immunoprecipitation - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555), expandable thumbnail

    Immunoprecipitation - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555)

    This data was developed using ab307554, the same antibody clone in a different buffer formulation.
    Swd2 was immunoprecipitated from 0.35 mg Mouse colon tissue lysate with ab307554 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307554 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
    Lane 1: Mouse colon tissue lysate
    Lane 2: ab307554 IP in Mouse colon tissue lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab307554 in mouse colon tissue lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 67 seconds

    All lanes: Immunoprecipitation - Anti-Swd2 antibody [EPR27034-63] (AB307554) at 1/30 dilution

    All lanes: Mouse colon tissue lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366) at 1/5000 dilution

    Exposure time: 67s

    Swd2 was immunoprecipitated from 0.35 mg Mouse colon tissue lysate with ab307554 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307554 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
    Lane 1: Mouse colon tissue lysate
    Lane 2: ab307554 IP in Mouse colon tissue lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab307554 in mouse colon tissue lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 67 seconds

  • Immunoprecipitation - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555), expandable thumbnail

    Immunoprecipitation - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555)

    This data was developed using ab307554, the same antibody clone in a different buffer formulation.
    Swd2 was immunoprecipitated from 0.35 mg Human colon tissue lysate with ab307554 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307554 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
    Lane 1: Human colon tissue lysate
    Lane 2: ab307554 IP in Human colon tissue lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab307554 in human colon tissue lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 67 seconds

    All lanes: Immunoprecipitation - Anti-Swd2 antibody [EPR27034-63] (AB307554) at 1/30 dilution

    All lanes: Human colon tissue lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366) at 1/5000 dilution

    Exposure time: 67s

    Swd2 was immunoprecipitated from 0.35 mg Human colon tissue lysate with ab307554 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307554 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
    Lane 1: Human colon tissue lysate
    Lane 2: ab307554 IP in Human colon tissue lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab307554 in human colon tissue lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 67 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555)

    This data was developed using ab307554, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Swd2 with ab307554 at 1/2000 (0.275 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on rat colon. The section was incubated with ab307554 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555)

    This data was developed using ab307554, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Swd2 with ab307554 at 1/2000 (0.275 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on mouse colon. The section was incubated with ab307554 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555)

    This data was developed using ab307554, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Swd2 with ab307554 at 1/500 (1.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on human colon cancer (PMID: 30008910). The section was incubated with ab307554 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555)

    This data was developed using ab307554, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Swd2 with ab307554 at 1/500 (1.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on human colon (PMID: 30008910). The section was incubated with ab307554 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunocytochemistry/ Immunofluorescence - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555)

    This data was developed using ab307554, the same antibody clone in a different buffer formulation.
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling Swd2 with ab307554 at 1/50 (11.0 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in HeLa cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555)

    This data was developed using ab307554, the same antibody clone in a different buffer formulation.
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Swd2 with ab307554 at 1/50 (11.0 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Flow Cytometry (Intracellular) - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555)

    This data was developed using ab307554, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Swd2 with ab307554 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Swd2 antibody [EPR27034-63] - BSA and Azide free (ab307555)

    This data was developed using ab307554, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling Swd2 with ab307554 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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