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AB188276

Anti-Swi6 antibody

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(6 Publications)

Rabbit Polyclonal SWI6 antibody. Suitable for IP, ChIP, WB and reacts with Schizosaccharomyces pombe samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Schizosaccharomyces pombe 972h- swi6.

View Alternative Names

SPAC664.01c, SPAC824.10c, swi6, Chromatin-associated protein swi6, swi6, Swi 6, SPAC664.01c, SPAC824.10c, Chromodomain protein Swi6, Chromatin associated protein swi6

1 Images
Western blot - Anti-Swi6 antibody (AB188276)
  • WB

Supplier Data

Western blot - Anti-Swi6 antibody (AB188276)

All lanes:

Western blot - Anti-Swi6 antibody (ab188276) at 1/2000 dilution

All lanes:

S. pombe Swi6 protein

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Schizosaccharomyces pombe

Applications

ChIP, WB, IP

applications

Immunogen

Recombinant Full Length Protein corresponding to Schizosaccharomyces pombe 972h- swi6.

P40381

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Primary Antibodies

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Biochemicals

AB143756

3-BrB-PP1, Akt inhibitor

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Schizosaccharomyces pombe": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
pH: 6 - 8.5 Preservative: 0.09% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Swi6 also known as Char9 or Split Ends is a protein recognized for its role in cell cycle regulation. This protein has a mass of approximately 89 kDa and primarily expresses in the nucleus of both yeast and certain eukaryotic cells. Swi6 functions as a regulatory subunit and plays an integral role in transcription regulation. Swi6 also contributes to modulating the timing and coordination of critical cell cycle events.
Biological function summary

Swi6 binds with transcription factors and contributes to the formation of cell cycle-dependent kinase complexes. It assists in the regulation of transcription when it acts as a part of the SBF and MBF complexes. These complexes are responsible for the transcriptional activation of genes necessary for G1/S transition during the cell cycle. The ability of Swi6 to selectively bind to specific DNA sequences makes it fundamental in controlling gene expression patterns.

Pathways

Swi6 operates within the cell cycle control pathways. It interacts with the retinoblastoma (Rb) protein to influence the G1 checkpoint control pathway. In yeast Swi6 is also part of the START checkpoint pathway ensuring proper DNA synthesis initiation. Its association with proteins like Cln3 and Cdc28 is central to its function in these pathways linking external growth cues to internal cell cycle progression mechanisms.

Swi6 impacts cancer progression and development. Its involvement with cell cycle regulation connects it to various cancer types. Aberrant expression or mutations in Swi6 can lead to dysregulation of cell division often associated with the development of malignant cells. The protein's interaction with the Rb protein is also significant in cancer biology as disruptions in this pathway are frequently observed in tumorigenesis notably in retinoblastoma and other cancers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Involved in the repression of the silent mating-type loci MAT2 and MAT3. May compact MAT2/3 into a heterochromatin-like conformation which represses the transcription of these silent cassettes.
See full target information swi6
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9384 PubMed39477922

2024

Clr4 ubiquitination and non-coding RNA mediate transcriptional silencing of heterochromatin via Swi6 phase separation.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun-Soo Kim,Benjamin Roche,Sonali Bhattacharjee,Leila Todeschini,An-Yun Chang,Christopher Hammell,André Verdel,Robert A Martienssen

eLife 13: PubMed38289024

2024

Heat stress-induced activation of MAPK pathway attenuates Atf1-dependent epigenetic inheritance of heterochromatin in fission yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Li Sun,Libo Liu,Chunlin Song,Yamei Wang,Quan-Wen Jin

Cell reports 37:109944 PubMed34731638

2021

The histone chaperone FACT facilitates heterochromatin spreading by regulating histone turnover and H3K9 methylation states.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Murawska,R A Greenstein,Tamas Schauer,Karl C F Olsen,Henry Ng,Andreas G Ladurner,Bassem Al-Sady,Sigurd Braun

International journal of molecular sciences 22: PubMed33440639

2021

Fission Yeast Methylenetetrahydrofolate Reductase Ensures Mitotic and Meiotic Chromosome Segregation Fidelity.

Applications

Unspecified application

Species

Unspecified reactive species

Kim Kiat Lim,Hwei Yee Teo,Yuan Yee Tan,Yi Bing Zeng,Ulysses Tsz Fung Lam,Mahesh Choolani,Ee Sin Chen

Current biology : CB 31:182-191.e5 PubMed33096036

2020

A Synthetic Approach to Reconstruct the Evolutionary and Functional Innovations of the Plant Histone Variant H2A.W.

Applications

Unspecified application

Species

Unspecified reactive species

Bingkun Lei,Matías Capella,Sean A Montgomery,Michael Borg,Akihisa Osakabe,Malgorzata Goiser,Abubakar Muhammad,Sigurd Braun,Frédéric Berger

EMBO reports 16:1673-87 PubMed26518661

2015

The Paf1 complex factors Leo1 and Paf1 promote local histone turnover to modulate chromatin states in fission yeast.

Applications

ChIP

Species

Schizosaccharomyces pombe

Laia Sadeghi,Punit Prasad,Karl Ekwall,Amikam Cohen,J Peter Svensson
View all publications
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Product promise

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