Rabbit Polyclonal Hemagglutinin antibody. Suitable for ELISA, WB and reacts with Influenza A samples.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Reactivity data
Select an application| ELISA | WB | |
|---|---|---|
| Influenza A | Expected | Tested |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Influenza A | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Influenza A | Dilution info - | Notes - |
Associated Products
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Target data
Function
Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
Alternative names
Hemagglutinin, HA
Recommended products
Rabbit Polyclonal Hemagglutinin antibody. Suitable for ELISA, WB and reacts with Influenza A samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Purification technique
- Affinity purification Immunogen
- Specificity
ab91530 is specific for the Swine Influenza A (H1N1) Hemagglutinin and will not recognize the corresponding Hemagglutinin from the seasonal H1N1 influenza virus.
- Concentration
- Purification notes
Affinity chromatography purified via peptide column.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
The genetic make-up of this swine flu virus is unlike any other: it is an H1N1 strain that combines a triple assortment first identified in 1998 including human, swine, and avian influenza with two new pig H3N2 virus genes from Eurasia, themselves of recent human origin.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Swine Influenza A Hemagglutinin often called HA is a surface glycoprotein on the influenza virus particularly H1N1 subtype. HA plays a central role in the initial attachment of the virus to the host cell by binding to sialic acid receptors. This binding facilitates the entry of the virus into the cell. Hemagglutinin weighs approximately 75 kDa. It is expressed on the envelope of the influenza virus and serves as an important antigenic target for immune response.
- Biological function summary
Swine Influenza A Hemagglutinin functions to mediate the fusion between the viral envelope and the host cell membrane enabling the release of viral RNA into the host cell. HA operates as part of the viral protein complex within the influenza virus and is essential for viral infectivity. Its ability to mutate and change antigenic properties makes it a constant subject of study in immunology and vaccine development.
- Pathways
Hemagglutinin directly interacts with the process of viral endocytosis and membrane fusion. It is a component of the viral life cycle pathway which includes the replication and assembly of viral particles. It works in concert with other viral proteins like neuraminidase which helps in the release of newly formed viral particles from the host cell. These pathways are critical for understanding how the influenza virus replicates and spreads in the host organism.
- Associated diseases and disorders
HA is primarily associated with Influenza illness outbreaks such as the H1N1 flu. This protein's ability to undergo frequent antigenic shifts and drifts complicates the development of effective vaccines making it a significant focus in flu research. Hemagglutinin's relationship with other proteins like neuraminidase contributes to the pathogenicity of flu viruses. Additionally it plays a role in cross-species transmission with strains such as G4 EA H1N1 raising concerns about potential pandemic sources.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Swine Influenza A Hemagglutinin antibody (ab91530)
All lanes: Western blot - Anti-Swine Influenza A Hemagglutinin antibody (ab91530) at 2 µg/mL
Lane 1: Seasonal Influenza A (H1N1) Hemagglutinin at 15 µg/mL
Lane 2: Swine Influenza A (H1N1) Hemagglutinin at 15 µg/mL
Predicted band size: 63 kDa
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