Rabbit Recombinant Monoclonal Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) (A/swine/Henan/SN10/2018) antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (AB308359), expandable thumbnail](https://content.abcam.com/products/images/swine-influenza-a-virus-g4-ea-h1n1-ha-hemagglutinin-antibody-hl1342-ab308359--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408112.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (AB308359)")
![Immunocytochemistry/ Immunofluorescence - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (AB308359), expandable thumbnail](https://content.abcam.com/products/images/swine-influenza-a-virus-g4-ea-h1n1-ha-hemagglutinin-antibody-hl1342-ab308359--immunocytochemistry-immunofluorescence-img408110.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (AB308359)")
![Western blot - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (AB308359), expandable thumbnail](https://content.abcam.com/products/images/swine-influenza-a-virus-g4-ea-h1n1-ha-hemagglutinin-antibody-hl1342-ab308359--western-blot-img408104.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (AB308359)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Transfected cell line | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info 1/500 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info 1/5000 | Notes - |
Alternative names
Swine-Origin Influenza A (H1N1) Hemagglutinin: S-OIV A Hemagglutinin, Swine flu H1, A
Recommended products
Rabbit Recombinant Monoclonal Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) (A/swine/Henan/SN10/2018) antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line samples.
Alternative names
Swine-Origin Influenza A (H1N1) Hemagglutinin: S-OIV A Hemagglutinin, Swine flu H1, A
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Carrier free
Yes
- Clone number
HL1342
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (ab308359), expandable thumbnail](https://content.abcam.com/products/images/swine-influenza-a-virus-g4-ea-h1n1-ha-hemagglutinin-antibody-hl1342-ab308359--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408112.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (ab308359)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (ab308359)
Immunohistochemical analysis of mock and Influenza A virus HA (A/swine/Henan/SN10/2018) transfected HEK293T cell FFPE Cell Pellet Block labeling Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin using ab308359 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
![Immunocytochemistry/ Immunofluorescence - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (ab308359), expandable thumbnail](https://content.abcam.com/products/images/swine-influenza-a-virus-g4-ea-h1n1-ha-hemagglutinin-antibody-hl1342-ab308359--immunocytochemistry-immunofluorescence-img408110.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (ab308359)")
Immunocytochemistry/ Immunofluorescence - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (ab308359)
For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) protein using ab308359 at 1/500 (green). Nuclei (blue) is stained with fluoroshield with DAPI.
![Western blot - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (ab308359), expandable thumbnail](https://content.abcam.com/products/images/swine-influenza-a-virus-g4-ea-h1n1-ha-hemagglutinin-antibody-hl1342-ab308359--western-blot-img408104.jpg "Western blot - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (ab308359)")
Western blot - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (ab308359)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1342] (ab308359) diluted at 1/5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (ab308359) at 1/5000 dilution
Lane 1: Non-transfected (–) 293T whole cell extracts at 30 µg
Lane 2: Transfected (+) 293T whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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