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AB308359

Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342]

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Rabbit Recombinant Monoclonal Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) (A/swine/Henan/SN10/2018) antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line - Swine Influenza A virus samples.
3 Images
Western blot - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (AB308359)
  • WB

Supplier Data

Western blot - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (AB308359)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1342] (ab308359) diluted at 1/5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (ab308359) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (AB308359)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (AB308359)

Immunohistochemical analysis of mock and Influenza A virus HA (A/swine/Henan/SN10/2018) transfected HEK293T cell FFPE Cell Pellet Block labeling Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin using ab308359 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (AB308359)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Swine Influenza A virus G4 EA H1N1 HA Hemagglutinin antibody [HL1342] (AB308359)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) protein using ab308359 at 1/500 (green). Nuclei (blue) is stained with fluoroshield with DAPI.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1342

Isotype

IgG

Carrier free

Yes

Reacts with

Swine Influenza A virus

Applications

IHC-P, ICC/IF, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Swine Influenza A virus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Swine Influenza A virus": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Swine Influenza A Hemagglutinin often called HA is a surface glycoprotein on the influenza virus particularly H1N1 subtype. HA plays a central role in the initial attachment of the virus to the host cell by binding to sialic acid receptors. This binding facilitates the entry of the virus into the cell. Hemagglutinin weighs approximately 75 kDa. It is expressed on the envelope of the influenza virus and serves as an important antigenic target for immune response.
Biological function summary

Swine Influenza A Hemagglutinin functions to mediate the fusion between the viral envelope and the host cell membrane enabling the release of viral RNA into the host cell. HA operates as part of the viral protein complex within the influenza virus and is essential for viral infectivity. Its ability to mutate and change antigenic properties makes it a constant subject of study in immunology and vaccine development.

Pathways

Hemagglutinin directly interacts with the process of viral endocytosis and membrane fusion. It is a component of the viral life cycle pathway which includes the replication and assembly of viral particles. It works in concert with other viral proteins like neuraminidase which helps in the release of newly formed viral particles from the host cell. These pathways are critical for understanding how the influenza virus replicates and spreads in the host organism.

HA is primarily associated with Influenza illness outbreaks such as the H1N1 flu. This protein's ability to undergo frequent antigenic shifts and drifts complicates the development of effective vaccines making it a significant focus in flu research. Hemagglutinin's relationship with other proteins like neuraminidase contributes to the pathogenicity of flu viruses. Additionally it plays a role in cross-species transmission with strains such as G4 EA H1N1 raising concerns about potential pandemic sources.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) (A/swine/Henan/SN10/2018)
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com