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Rabbit Recombinant Monoclonal Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) (A/swine/Henan/SN10/2018) antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line samples.

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Images

Western blot - Anti-Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] (AB308395), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] (AB308395), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] (AB308395), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWB
Transfected cell line
Tested
Tested
Tested

Tested
Tested

Species
Transfected cell line
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Transfected cell line
Dilution info
1/500
Notes

-

Tested
Tested

Species
Transfected cell line
Dilution info
1/5000
Notes

-

Target data

Alternative names

Swine-Origin Influenza A (H1N1) Hemagglutinin: S-OIV A Hemagglutinin, Swine flu H1, A

Rabbit Recombinant Monoclonal Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) (A/swine/Henan/SN10/2018) antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Carrier free
Yes
Clone number
HL1343
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] (ab308395), expandable thumbnail

    Western blot - Anti-Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] (ab308395)

    Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] (ab308395) diluted at 1/5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] (ab308395) at 1/5000 dilution

    Lane 1: Non-transfected (–) 293T whole cell extracts at 30 µg

    Lane 2: Transfected (+) 293T whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] (ab308395), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] (ab308395)

    Immunohistochemical analysis of mock and Influenza A virus HA (A/swine/Henan/SN10/2018) transfected HEK293T cell FFPE Cell Pellet Block labeling Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) (green) using ab308395 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

  • Immunocytochemistry/ Immunofluorescence - Anti-Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] (ab308395), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] (ab308395)

    For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) using ab308395 at 1/500 dilution (green). Blue: Fluoroshield with DAPI.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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