AB36195

Anti-SYBL1/VAMP-7 antibody [158.2]

Name: Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195) | Abcam
Brand: Abcam Limited
SKU: AB36195
Rating: 5 (1 reviews)

(1 Review)

(30 Publications)

Mouse Monoclonal SYBL1/VAMP-7 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Rat, Human samples. Cited in 30 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VAMP7.

View Alternative Names

SYBL1, VAMP7, Vesicle-associated membrane protein 7, VAMP-7, Synaptobrevin-like protein 1, Tetanus-insensitive VAMP, Ti-VAMP

2 Images

Flow Cytometry - Anti-SYBL1/VAMP-7 antibody [158.2] (AB36195)

Flow Cyt

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Flow Cytometry - Anti-SYBL1/VAMP-7 antibody [158.2] (AB36195)

Overlay histogram showing PC12 cells stained with ab36195 (red line). The cells were fixed with 4% paraformaldehyde and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36195, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

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Western blot - Anti-SYBL1/VAMP-7 antibody [158.2] (AB36195)

All lanes:

Western blot - Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195) at 1/500 dilution

All lanes:

Tissue lysates prepared from rat brain

Predicted band size: 25 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

158.2

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF, IHC-Fr, WB, IHC-P, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human VAMP7.

P51809

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.

See full target information VAMP7

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Publications (30)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 44:534-562 PubMed39653855

2024

SNARE mimicry by the CD225 domain of IFITM3 enables regulation of homotypic late endosome fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Kazi Rahman,Isaiah Wilt,Abigail A Jolley,Bhabadeb Chowdhury,Siddhartha A K Datta,Alex A Compton

The Journal of biological chemistry 300:107911 PubMed39433126

2024

Lysosomal activity depends on TRPML1-mediated Ca release coupled to incoming vesicle fusions.

Applications

Unspecified application

Species

Unspecified reactive species

Arindam Bhattacharjee,Hussein Abuammar,Gábor Juhász

Cancer gene therapy 30:149-162 PubMed36123390

2022

YAP 5-methylcytosine modification increases its mRNA stability and promotes the transcription of exosome secretion-related genes in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Yu,Congcong Zhang,Yikun Wang,Xiaoting Tian,Yayou Miao,Fanyu Meng,Lifang Ma,Xiao Zhang,Jinjing Xia

Cell chemical biology 29:1419-1433.e5 PubMed35738280

2022

A genome-wide CRISPR-Cas9 screen identifies CENPJ as a host regulator of altered microtubule organization during Plasmodium liver infection.

Applications

Unspecified application

Species

Unspecified reactive species

Kamalakannan Vijayan,Nadia Arang,Ling Wei,Robert Morrison,Rechel Geiger,K Rachael Parks,Adam J Lewis,Fred D Mast,Alyse N Douglass,Heather S Kain,John D Aitchison,Jarrod S Johnson,Alan Aderem,Alexis Kaushansky

Communications biology 4:1091 PubMed34531530

2021

TC10 regulates breast cancer invasion and metastasis by controlling membrane type-1 matrix metalloproteinase at invadopodia.

Applications

Unspecified application

Species

Unspecified reactive species

Maren Hülsemann,Colline Sanchez,Polina V Verkhusha,Vera Des Marais,Serena P H Mao,Sara K Donnelly,Jeffrey E Segall,Louis Hodgson

Traffic (Copenhagen, Denmark) 22:368-376 PubMed34476885

2021

The trans-SNARE complex VAMP4/Stx6/Stx7/Vti1b is a key regulator of Golgi to late endosome MT1-MMP transport in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Zoe Elizabeth West,Savannah Margaret Aitcheson,Annalese Barbara Trudy Semmler,Rachael Zoe Murray

Traffic (Copenhagen, Denmark) 20:661-673 PubMed31297933

2019

Invasion by activated macrophages requires delivery of nascent membrane-type-1 matrix metalloproteinase through late endosomes/lysosomes to the cell surface.

Applications

Unspecified application

Species

Unspecified reactive species

Joan Röhl,Zoe E West,Maren Rudolph,Andreea Zaharia,Derek Van Lonkhuyzen,Danica K Hickey,Annalese B T Semmler,Rachael Z Murray

Autophagy 16:797-810 PubMed31251111

2019

DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Tian,Pengli Zheng,Chenqian Zhou,Xurui Wang,Hua Ma,Wei Ma,Xiaokai Zhou,Junlin Teng,Jianguo Chen

The Journal of neuroscience : the official journal 39:6049-6066 PubMed31160536

2019

Ablation of All Synaptobrevin vSNAREs Blocks Evoked But Not Spontaneous Neurotransmitter Release at Neuromuscular Synapses.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Liu,Yoshie Sugiura,Thomas C Südhof,Weichun Lin

Neuron 102:1184-1198.e10 PubMed31072786

2019

Activity-Dependent Secretion of Synaptic Organizer Cbln1 from Lysosomes in Granule Cell Axons.

Applications

Unspecified application

Species

Unspecified reactive species

Keiji Ibata,Maya Kono,Sakae Narumi,Junko Motohashi,Wataru Kakegawa,Kazuhisa Kohda,Michisuke Yuzaki

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SYBL1/VAMP-7 antibody [158.2]

Flow Cytometry - Anti-SYBL1/VAMP-7 antibody [158.2] (AB36195)

Western blot - Anti-SYBL1/VAMP-7 antibody [158.2] (AB36195)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Target data

Publications (30)

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