Mouse Monoclonal SYBL1/VAMP-7 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Rat, Human samples. Cited in 26 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VAMP7.
Images
![Western blot - Anti-SYBL1/VAMP-7 antibody [158.2] (AB36195), expandable thumbnail](https://content.abcam.com/products/images/sybl1-vamp-7-antibody-1582-ab36195--western-blot-img96961.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SYBL1/VAMP-7 antibody [158.2] (AB36195)")
![Flow Cytometry - Anti-SYBL1/VAMP-7 antibody [158.2] (AB36195), expandable thumbnail](https://content.abcam.com/products/images/sybl1-vamp-7-antibody-1582-ab36195--flow-cytometry-img80258.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-SYBL1/VAMP-7 antibody [158.2] (AB36195)")
Publications
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- Cell chemical biology 29:1419-1433.e52022A genome-wide CRISPR-Cas9 screen identifies CENPJ as a host regulator of altered microtubule organization during Plasmodium liver infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKamalakannan Vijayan,Nadia Arang,Ling Wei,Robert Morrison,Rechel Geiger,K Rachael Parks,Adam J Lewis,Fred D Mast,Alyse N Douglass,Heather S Kain,John D Aitchison,Jarrod S Johnson,Alan Aderem,Alexis KaushanskyPubMed 35738280
- Communications biology 4:10912021TC10 regulates breast cancer invasion and metastasis by controlling membrane type-1 matrix metalloproteinase at invadopodia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaren Hülsemann,Colline Sanchez,Polina V Verkhusha,Vera Des Marais,Serena P H Mao,Sara K Donnelly,Jeffrey E Segall,Louis HodgsonPubMed 34531530
- Traffic (Copenhagen, Denmark) 22:368-3762021The trans-SNARE complex VAMP4/Stx6/Stx7/Vti1b is a key regulator of Golgi to late endosome MT1-MMP transport in macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZoe Elizabeth West,Savannah Margaret Aitcheson,Annalese Barbara Trudy Semmler,Rachael Zoe MurrayPubMed 34476885
- Traffic (Copenhagen, Denmark) 20:661-6732019Invasion by activated macrophages requires delivery of nascent membrane-type-1 matrix metalloproteinase through late endosomes/lysosomes to the cell surface.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoan Röhl,Zoe E West,Maren Rudolph,Andreea Zaharia,Derek Van Lonkhuyzen,Danica K Hickey,Annalese B T Semmler,Rachael Z MurrayPubMed 31297933
- Autophagy 16:797-8102019DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyu Tian,Pengli Zheng,Chenqian Zhou,Xurui Wang,Hua Ma,Wei Ma,Xiaokai Zhou,Junlin Teng,Jianguo ChenPubMed 31251111
- The Journal of neuroscience : the official journal 39:6049-60662019Ablation of All Synaptobrevin vSNAREs Blocks Evoked But Not Spontaneous Neurotransmitter Release at Neuromuscular Synapses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Liu et. al.PubMed 31160536
- Oncotarget 8:110994-1110112017C3G promotes a selective release of angiogenic factors from activated mouse platelets to regulate angiogenesis and tumor metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVíctor Martín-Granado et. al.PubMed 29340032
- Cellular microbiology 19:2017Soluble N-ethylmaleimide-sensitive factor attachment protein receptors required during Trypanosoma cruzi parasitophorous vacuole development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan Agustín Cueto,María Cristina Vanrell,Betiana Nebaí Salassa,Sébastien Nola,Thierry Galli,María Isabel Colombo,Patricia Silvia RomanoPubMed 27992096
- The Journal of cell biology 211:587-6042015Cargo-selective apical exocytosis in epithelial cells is conducted by Myo5B, Slp4a, Vamp7, and Syntaxin 3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeorg F Vogel,Katharina M C Klee,Andreas R Janecke,Thomas Müller,Michael W Hess,Lukas A HuberPubMed 26553929
- The Journal of biological chemistry 289:33513-282014Myosin vc interacts with Rab32 and Rab38 proteins and works in the biogenesis and secretion of melanosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJarred J Bultema et. al.PubMed 25324551
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human VAMP7. Database link P51809
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|
| Human | Predicted | Predicted | Expected | Expected | Expected |
| Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rat | Tested | Tested | Expected | Expected | Expected |
| Dog | Predicted | Predicted | Predicted | Predicted | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/50 | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog, Human | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.
Alternative names
SYBL1, VAMP7, Vesicle-associated membrane protein 7, VAMP-7, Synaptobrevin-like protein 1, Tetanus-insensitive VAMP, Ti-VAMP
Recommended products
Mouse Monoclonal SYBL1/VAMP-7 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Rat, Human samples. Cited in 26 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VAMP7.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human VAMP7. Database link P51809
- Clone number
- 158.2
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195), expandable thumbnail](https://content.abcam.com/products/images/sybl1-vamp-7-antibody-1582-ab36195--western-blot-img96961.jpg "Western blot - Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195)")
Western blot - Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195)
All lanes: Western blot - Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195) at 1/500 dilution
All lanes: Tissue lysates prepared from rat brain
Predicted band size: 25 kDa
![Flow Cytometry - Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195), expandable thumbnail](https://content.abcam.com/products/images/sybl1-vamp-7-antibody-1582-ab36195--flow-cytometry-img80258.jpg "Flow Cytometry - Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195)")
Flow Cytometry - Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195)
Overlay histogram showing PC12 cells stained with ab36195 (red line). The cells were fixed with 4% paraformaldehyde and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36195, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.
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