Rabbit Polyclonal SYBL1/VAMP-7 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human VAMP7 aa 100-200.
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Publications
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- eLife 9:2020Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlex J B Kreutzberger,Volker Kiessling,Catherine A Doyle,Noah Schenk,Clint M Upchurch,Margaret Elmer-Dixon,Amanda E Ward,Julia Preobraschenski,Syed S Hussein,Weronika Tomaka,Patrick Seelheim,Iman Kattan,Megan Harris,Binyong Liang,Anne K Kenworthy,Bimal N Desai,Norbert Leitinger,Arun Anantharam,J David Castle,Lukas K TammPubMed 33164744
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human VAMP7 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link P51809
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Orangutan | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.
Alternative names
SYBL1, VAMP7, Vesicle-associated membrane protein 7, VAMP-7, Synaptobrevin-like protein 1, Tetanus-insensitive VAMP, Ti-VAMP
Recommended products
Rabbit Polyclonal SYBL1/VAMP-7 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human VAMP7 aa 100-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human VAMP7 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link P51809
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-SYBL1/VAMP-7 antibody (ab224535)
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for SYBL1/VAMP-7 (green) using ab224535 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYBL1/VAMP-7 antibody (ab224535)
Paraffin-embedded human pancreas tissue stained for SYBL1/VAMP-7 using ab224535 at 1/50 dilution in immunohistochemical analysis.
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