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Rabbit Recombinant Monoclonal Syk phospho Y323 antibody. Suitable for IHC-P, Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide samples. Cited in 11 publications.

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Images

Western blot - Anti-Syk (phospho Y323) antibody [EP573-4] (AB62338), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Syk (phospho Y323) antibody [EP573-4] (AB62338), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk (phospho Y323) antibody [EP573-4] (AB62338), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Syk (phospho Y323) antibody [EP573-4] (AB62338), expandable thumbnail
  • Dot Blot - Anti-Syk (phospho Y323) antibody [EP573-4] (AB62338), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPDotWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Expected
Tested
Tested
Tested
Synthetic peptide
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/2000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Associated Products

Select an associated product type

10 products for Alternative Product

1 product for Alternative Version

Target data

Function

Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development (PubMed:12387735, PubMed:33782605). Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include DEPTOR, VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK (PubMed:12456653, PubMed:15388330, PubMed:34634301, PubMed:8657103). Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition (PubMed:12456653). Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors. It also phosphorylates and activates PLCG1 and the PKC signaling pathway. It also phosphorylates BTK and regulates its activity in B-cell antigen receptor (BCR)-coupled signaling. In addition to its function downstream of BCR also plays a role in T-cell receptor signaling. Plays also a crucial role in the innate immune response to fungal, bacterial and viral pathogens. It is for instance activated by the membrane lectin CLEC7A. Upon stimulation by fungal proteins, CLEC7A together with SYK activates immune cells inducing the production of ROS. Also activates the inflammasome and NF-kappa-B-mediated transcription of chemokines and cytokines in presence of pathogens. Regulates neutrophil degranulation and phagocytosis through activation of the MAPK signaling cascade (By similarity). Required for the stimulation of neutrophil phagocytosis by IL15 (PubMed:15123770). Also mediates the activation of dendritic cells by cell necrosis stimuli. Also involved in mast cells activation. Involved in interleukin-3/IL3-mediated signaling pathway in basophils (By similarity). Also functions downstream of receptors mediating cell adhesion (PubMed:12387735). Relays for instance, integrin-mediated neutrophils and macrophages activation and P-selectin receptor/SELPG-mediated recruitment of leukocytes to inflammatory loci. Also plays a role in non-immune processes. It is for instance involved in vascular development where it may regulate blood and lymphatic vascular separation. It is also required for osteoclast development and function. Functions in the activation of platelets by collagen, mediating PLCG2 phosphorylation and activation. May be coupled to the collagen receptor by the ITAM domain-containing FCER1G. Also activated by the membrane lectin CLEC1B that is required for activation of platelets by PDPN/podoplanin. Involved in platelet adhesion being activated by ITGB3 engaged by fibrinogen. Together with CEACAM20, enhances production of the cytokine CXCL8/IL-8 via the NFKB pathway and may thus have a role in the intestinal immune response (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Syk phospho Y323 antibody. Suitable for IHC-P, Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide samples. Cited in 11 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP573-4

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Spleen tyrosine kinase commonly known as Syk is a non-receptor tyrosine kinase with a mass of about 72 kDa. It serves as a critical signaling molecule in immune cells. Syk is expressed in various cell types such as B cells T cells monocytes and others involved in the immune response. It initiates signaling cascades by binding to immunoreceptor tyrosine-based activation motifs (ITAMs). Alternate forms include phosphorylated Syk often referred to as p-Syk which activates various downstream signaling pathways.

Biological function summary

Syk plays a role in transmitting signals from the cell surface to the interior of the cell facilitating various cellular activities. It is a part of signal transduction complexes and functions in the regulation of immune cell activation differentiation and survival. It affects processes like Fc receptor signaling in macrophages and neutrophils impacting immune responses and inflammation. The phospho-Syk form influences the strength and duration of signaling affecting various cellular functions.

Pathways

Syk is significant in the B-cell receptor (BCR) signaling pathway and Fc receptor signaling pathway. Within these pathways Syk interacts with proteins like Lyn and Fyn which are other kinases involved in BCR signal transduction. Syk phosphorylation noted as p-Syk plays a part in activating downstream molecules such as phospholipase C gamma (PLCγ) to mediate cellular responses to external stimuli. The critical role of Syk in these pathways influences both adaptive and innate immune responses.

Associated diseases and disorders

Syk correlates with conditions like rheumatoid arthritis and certain B-cell lymphomas. In rheumatoid arthritis aberrant Syk signaling contributes to the inflammation and joint destruction characteristic of the disease. Syk's role in B-cell antigen receptor signaling links it to B-cell lymphomas where dysregulation in signaling pathways leads to uncontrolled proliferation of malignant B cells. The involvement of Syk in both disease contexts suggests its potential as a therapeutic target along with pathways related to proteins like BLNK and PLCγ in these disorders.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338), expandable thumbnail

    Western blot - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338)

    Beta tubulin shows equal lysate loading amount per lane.

    All lanes: Western blot - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338) at 1/2000 dilution

    Lane 1: untreated U937 cell lysates at 10 µg

    Lane 2: U937 cell lysates treated with pervanadate. at 10 µg

    Secondary

    All lanes: goat anti-rabbit, HRP labeled, at 1/2000 dilution

    Predicted band size: 72 kDa

    Observed band size: 72 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338)

    Immunocytochemistry/Immunofluorescence analysis of Ramos+/-pervanadate(1mM, 30min), Ramos + pervanadate(1mM, 30min) + LP labelling Syk (phospho Y323) with ab62338 at a dilution of 1:1000 dilution (2.53ug/ml). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) (1:1000) was used as the secondary antibody. The cells were co-stained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338)

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue sections labeling Syk with purified ab62338 at 1/100 dilution. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Sections were counterstained with Hematoxylin.
    Antigen retrieval was heat mediated antigen retrieval using citrate buffer, pH 6.0).

  • Flow Cytometry (Intracellular) - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338)

    Intracellular Flow Cytometry analysis of U937 (human monocyte histiocytic lymphoma) treated (red)/untreated (green) with 1mM pervanadate for 30 minutes with purified ab62338 at 1/2500 dilution. The secondary antibody was Goat anti rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

  • Dot Blot - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338), expandable thumbnail

    Dot Blot - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338)

    Dot blot analysis of Sky (pY323) peptide (Lane 1), Syk (unmodified) peptide (Lane 2), labelling Syk (pY323) with ab62338 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Western blot - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338), expandable thumbnail

    Western blot - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338)

    All lanes: Western blot - Anti-Syk (phospho Y323) antibody [EP573-4] (ab62338) at 1/5000 dilution

    Lane 1: Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 10 µg

    Lane 2: Ramos treated with 1mM pervanadate for 30min whole cell lysate at 10 µg

    Lane 3: Ramos treated with 1mM pervanadate for 30min whole cell lysate. Then the membrane was incubated with alkaline phosphatase. at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 72 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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