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AB300410

Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Syk phospho Y319 antibody. Carrier free. Suitable for WB, Dot, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Synthetic peptide - Human, Synthetic peptide - Mouse samples. Cited in 1 publication.

View Alternative Names

Tyrosine-protein kinase SYK, Spleen tyrosine kinase, p72-Syk, SYK

9 Images
Immunocytochemistry/ Immunofluorescence - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)

This data was developed using ab300398, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat (human T cell leukemia T lymphocyte) cells labelling Syk (phospho Y352) +ZAP70 (phospho Y319) with ab300398 at 1/50 (9.44 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing increased membranous staining in Jurkat cells treated with pervanadate (50 mM) for 5 min is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Flow Cytometry (Intracellular) - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)

This data was developed using ab300398, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) treated with 1mM pervanadate for 30 minutes (Red)/ Untreated control (Green) cells labelling Syk (phospho Y352) +ZAP70 (phospho Y319) with ab300398 at 1/500 dilution (0.1ug) (Red) and Green (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)

This data was developed using ab300398, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Panel A, Mouse splee tissue labeling Syk (phospho Y352) +ZAP70 (phospho Y319) with ab300398 at 1/500 (0.944 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse spleen without alkaline phosphatase treatment (Panel A); no staining on mouse spleen with alkaline phosphatase treatment (Panel B). The section was incubated with ab300398 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Flow Cytometry (Intracellular) - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)

This data was developed using ab300398, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized EL4 (mouse lymphoma t lymphocyte) treated with 11mM H2O2 for 10 minutes then 10mM pervanadate for 30 minutes (Red)/ Untreated control (Green) cells labelling Syk (phospho Y352) +ZAP70 (phospho Y319) with ab300398 at 1/50 dilution (1ug) (Red) and Green (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)

This data was developed using ab300398, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labelling Syk (phospho Y352) +ZAP70 (phospho Y319) with ab300398 at 1/50 (9.44 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing increased membranous staining in EL4 cells treated with H2O2 (11 mM) for 10 min and then pervanadate (10 mM) for 30 min is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)

This data was developed using ab300398, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Panel A, Mouse pancr tissue labeling Syk (phospho Y352) +ZAP70 (phospho Y319) with ab300398 at 1/500 (0.944 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse pancreatic cancer without alkaline phosphatase treatment (Panel A); no staining on mouse pancreatic cancer with alkaline phosphatase treatment (Panel B). The section was incubated with ab300398 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)

This data was developed using ab300398, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Panel A, Mouse lung tissue labeling Syk (phospho Y352) +ZAP70 (phospho Y319) with ab300398 at 1/500 (0.944 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse lung without alkaline phosphatase treatment (Panel A); no staining on mouse lung with alkaline phosphatase treatment (Panel B). The section was incubated with ab300398 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Western blot - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)
  • WB

Supplier Data

Western blot - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)

This data was developed using ab300398, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer and concentration : 5% NFDM/TBST

Exposure time : Lanes 1-4 : 6 seconds; Lanes 5-6 : 147 seconds.

All lanes:

Western blot - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] (<a href='/en-us/products/primary-antibodies/syk-phospho-y352-zap70-phospho-y319-antibody-epr26232-39-ab300398'>ab300398</a>) at 1/1000 dilution

Lane 1:

Untreated Jurkat whole cell lysate at 20 µg

Lane 2:

Jurkat treated with 50mM pervanadate for 5 minutes whole cell lysate at 20 µg

Lane 3:

ntreated starved 24 hours Ramos whole cell lysate at 20 µg

Lane 4:

Ramos starved 24 hours, then treated with 10mM pervanadate for 30 minuteswhole cell lysate at 20 µg

Lane 5:

Untreated EL4 whole cell lysate at 20 µg

Lane 6:

EL4 treated with 11mM H2O2 for 10 minutes, then 10mM pervanadate for 30 minutes whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa,72 kDa

false

Dot Blot - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)
  • Dot

Supplier Data

Dot Blot - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] - BSA and Azide free (AB300410)

This data was developed using ab300398, the same antibody clone in a different buffer formulation.

Blocking/diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Dot Blot - Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39] (<a href='/en-us/products/primary-antibodies/syk-phospho-y352-zap70-phospho-y319-antibody-epr26232-39-ab300398'>ab300398</a>) at 1/1000 dilution

Lane 1:

Syk (phospho Y352) peptide a

Lane 2:

Syk (phospho Y352) peptide b

Lane 3:

Syk non-phospho peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

false

Exposure time: 180s

  • Unconjugated

    Anti-Syk (phospho Y352) +ZAP70 (phospho Y319) antibody [EPR26232-39]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26232-39

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

WB, IHC-P, Flow Cyt (Intra), ICC/IF, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Not suitable for human</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Not suitable for human</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Not suitable for human</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Synthetic peptide - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Synthetic peptide - Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Syk (spleen tyrosine kinase) and ZAP70 (zeta-chain-associated protein kinase 70) are non-receptor tyrosine kinases. Syk has a molecular mass of approximately 72 kDa while ZAP70 is around 70 kDa. They are expressed in hematopoietic tissues. Syk is found in B-cells and other myeloid lineages and ZAP70 is mainly located in T-cells and natural killer (NK) cells. These kinases play essential roles in signal transduction by phosphorylating proteins on tyrosine residues transmitting activation signals from receptors on the cell surface to the interior of the cell.
Biological function summary

Syk and ZAP70 play vital roles in the adaptive immune response. Syk is an essential mediator of B-cell receptor (BCR) signaling and assembles into a signaling complex with other proteins. ZAP70 functions similarly in T-cells becoming activated upon the engagement of the T-cell receptor (TCR). Both kinases help recruit and activate adaptor proteins and downstream signaling molecules that drive cellular responses including proliferation differentiation and survival.

Pathways

Both kinases are integral to the B-cell and T-cell receptor signaling pathways. Syk activates several pathways including the MAP kinase and NF-kB pathways through its interaction with proteins like BLNK and PLCγ2. ZAP70 initiates the TCR signaling cascade interfacing with LAT and SLP-76 to activate the same pathways. These pathways trigger transcriptional programs necessary for immune cell functions and development.

Syk and ZAP70 have connections to various immune-related conditions. Abnormal Syk activity relates to autoimmune diseases like rheumatoid arthritis where B-cell signaling plays a role. Syk interacts with proteins such as ITAM-containing immunoreceptors in these disorders. ZAP70 mutations are linked to immunodeficiency syndromes leading to dysfunctional T-cell signaling. These kinases make viable targets for therapeutic intervention bringing hope for better management of such immune diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development (PubMed : 12387735, PubMed : 33782605). Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include DEPTOR, VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK (PubMed : 12456653, PubMed : 15388330, PubMed : 34634301, PubMed : 8657103). Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition (PubMed : 12456653). Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors. It also phosphorylates and activates PLCG1 and the PKC signaling pathway. It also phosphorylates BTK and regulates its activity in B-cell antigen receptor (BCR)-coupled signaling. In addition to its function downstream of BCR also plays a role in T-cell receptor signaling. Also plays a crucial role in the innate immune response to fungal, bacterial and viral pathogens. It is for instance activated by the membrane lectin CLEC7A. Upon stimulation by fungal proteins, CLEC7A together with SYK activates immune cells inducing the production of ROS. Also activates the inflammasome and NF-kappa-B-mediated transcription of chemokines and cytokines in presence of pathogens. Regulates neutrophil degranulation and phagocytosis through activation of the MAPK signaling cascade (By similarity). Required for the stimulation of neutrophil phagocytosis by IL15 (PubMed : 15123770). Also mediates the activation of dendritic cells by cell necrosis stimuli. Also involved in mast cells activation. Involved in interleukin-3/IL3-mediated signaling pathway in basophils (By similarity). Also functions downstream of receptors mediating cell adhesion (PubMed : 12387735). Relays for instance, integrin-mediated neutrophils and macrophages activation and P-selectin receptor/SELPG-mediated recruitment of leukocytes to inflammatory loci. Also plays a role in non-immune processes. It is for instance involved in vascular development where it may regulate blood and lymphatic vascular separation. It is also required for osteoclast development and function. Functions in the activation of platelets by collagen, mediating PLCG2 phosphorylation and activation. May be coupled to the collagen receptor by the ITAM domain-containing FCER1G. Also activated by the membrane lectin CLEC1B that is required for activation of platelets by PDPN/podoplanin. Involved in platelet adhesion being activated by ITGB3 engaged by fibrinogen. Together with CEACAM20, enhances production of the cytokine CXCL8/IL-8 via the NFKB pathway and may thus have a role in the intestinal immune response (By similarity).
See full target information SYK pY319
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastroenterology 28:5865-5880 PubMed36353208

2022

Curcumin alleviates experimental colitis a potential mechanism involving memory B cells and Bcl-6-Syk-BLNK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Yi Wei,Tian-Tian Wu,Jia-Qi Huang,Zeng-Ping Kang,Meng-Xue Wang,You-Bao Zhong,Wei Ge,Bu-Gao Zhou,Hai-Mei Zhao,Hai-Yan Wang,Duan-Yong Liu
View all publications
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Product promise

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