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AB251887

Anti-Symplekin antibody

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Rabbit Polyclonal Symplekin antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SYMPK aa 100-250.

View Alternative Names

SPK, SYMPK, Symplekin

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Symplekin antibody (AB251887)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Symplekin antibody (AB251887)

PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells stained for Symplekin (green) using ab251887 at 4 µg/ml in ICC/IF.

Western blot - Anti-Symplekin antibody (AB251887)
  • WB

Unknown

Western blot - Anti-Symplekin antibody (AB251887)

All lanes:

Western blot - Anti-Symplekin antibody (ab251887) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) whole cell lysate

Predicted band size: 141 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SYMPK aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q92797

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Symplekin is a multifunctional protein essential for mRNA processing. It has a molecular mass of approximately 150 kDa and is alternatively known as SYM or SPK. You can find Symplekin expressed in the nucleus of eukaryotic cells where it acts mainly at the 3' end of mRNAs. This protein interacts with other molecules in the cell through its well-defined domains that facilitate its function in RNA processing.
Biological function summary

Symplekin modulates the stability and maturation of mRNA transcripts. It acts as a scaffold protein assembling components of the cleavage and polyadenylation specificity factor (CPSF) complex. Within this complex Symplekin collaborates with other proteins like CPSF160 and CPSF73 to regulate the addition of the poly(A) tail important for mRNA stability transport and translation. This function supports the processing of pre-mRNA into mature mRNA.

Pathways

Symplekin participates in important gene expression regulation. It plays a role in the mRNA processing and gene expression pathways. Symplekin interacts with proteins involved in these pathways like CPSF and other RNA-binding proteins ensuring accurate mRNA maturation and stability. These coordinated activities are vital for the regulation of gene expression and by extension cell function and viability.

Symplekin shows association with conditions related to abnormal mRNA processing. Dysregulation of Symplekin can have impacts on cellular processes and has been implicated in cancer and neurodegenerative diseases. In cancer improper mRNA processing and polyadenylation can lead to abnormal cell proliferation and tumor growth with Symplekin often interacting with oncogenic regulatory proteins. In neurodegenerative diseases such as Alzheimer's disruptions involving Symplekin might affect neural mRNA stability linking it to amyloid precursor protein processing.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Scaffold protein that functions as a component of a multimolecular complex involved in histone mRNA 3'-end processing. Specific component of the tight junction (TJ) plaque, but might not be an exclusively junctional component. May have a house-keeping rule. Is involved in pre-mRNA polyadenylation. Enhances SSU72 phosphatase activity.
See full target information SYMPK
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Product promise

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