AB76494

Anti-SYN2 antibody [EPR3277]

RabMAb
Recombinant
What is this?

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(12 Publications)

Rabbit Recombinant Monoclonal SYN2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 12 publications.

View Alternative Names

Synapsin-2, Synapsin II, SYN2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYN2 antibody [EPR3277] (AB76494)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYN2 antibody [EPR3277] (AB76494)

Immunohistochemical analysis of paraffin-embedded human brain tissue using 1/100 ab76494

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-SYN2 antibody [EPR3277] (AB76494)

All lanes:

Western blot - Anti-SYN2 antibody [EPR3277] (ab76494) at 1/20000 dilution

All lanes:

Fetal brain tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/1000 dilution

Predicted band size: 63 kDa

Observed band size: 75 kDa

false

Carrier free

Anti-SYN2 antibody [EPR3277] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3277

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. May play a role in noradrenaline secretion by sympathetic neurons (By similarity).

See full target information Synapsin-2

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Aging 13:23739-23756 PubMed34689137

2021

Loganin substantially ameliorates molecular deficits, pathologies and cognitive impairment in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Lulin Nie,Kaiwu He,Fengzhu Xie,Shifeng Xiao,Shupeng Li,Jia Xu,Kaiqin Zhang,Chen Yang,Li Zhou,Jianjun Liu,Liangyu Zou,Xifei Yang

Scientific reports 11:14001 PubMed34234233

2021

The UL16 protein of HSV-1 promotes the metabolism of cell mitochondria by binding to ANT2 protein.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyu Li,Shuting Liu,Zhenning Dai,Qian Zhang,Yichao Xu,Youyu Chen,Zhenyou Jiang,Wenhua Huang,Hanxiao Sun

iScience 24:102504 PubMed34113835

2021

Gene-environment interactions mediate stress susceptibility and resilience through the CaMKIIβ/TARPγ-8/AMPAR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Sakai,Haiyan Li,Hiromichi Inaba,Yuki Funayama,Erina Ishimori,Ayako Kawatake-Kuno,Hirotaka Yamagata,Tomoe Seki,Teruyuki Hobara,Shin Nakagawa,Yoshifumi Watanabe,Susumu Tomita,Toshiya Murai,Shusaku Uchida

Oxidative medicine and cellular longevity 2020:5408452 PubMed32587661

2020

Proteomic Profile of Mouse Brain Aging Contributions to Mitochondrial Dysfunction, DNA Oxidative Damage, Loss of Neurotrophic Factor, and Synaptic and Ribosomal Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Yingchao Li,Haitao Yu,Chongyang Chen,Shupeng Li,Zaijun Zhang,Hua Xu,Feiqi Zhu,Jianjun Liu,Peter S Spencer,Zhongliang Dai,Xifei Yang

General physiology and biophysics 38:379-388 PubMed31411574

2019

Trafficking of synaptic vesicles is changed at the hypothalamus by exposure to an 835 MHz radiofrequency electromagnetic field.

Applications

Unspecified application

Species

Unspecified reactive species

Ju Hwan Kim,Yang Hoon Huh,Hak Rim Kim

Scientific reports 9:1201 PubMed30718744

2019

Decreased dopamine in striatum and difficult locomotor recovery from MPTP insult after exposure to radiofrequency electromagnetic fields.

Applications

Unspecified application

Species

Unspecified reactive species

Ju Hwan Kim,Choong-Hyun Lee,Hyung-Gun Kim,Hak Rim Kim

Food & function 9:1532-1544 PubMed29431797

2018

Sustained consumption of cocoa-based dark chocolate enhances seizure-like events in the mouse hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Cicvaric,Tanja Bulat,Daniel Bormann,Jiaye Yang,Bastian Auer,Ivan Milenkovic,Maureen Cabatic,Radoslav Milicevic,Francisco J Monje

PloS one 12:e0186416 PubMed29045446

2017

Changes in numbers and size of synaptic vesicles of cortical neurons induced by exposure to 835 MHz radiofrequency-electromagnetic field.

Applications

Unspecified application

Species

Unspecified reactive species

Ju Hwan Kim,Hyo-Jeong Kim,Da-Hyeon Yu,Hee-Seok Kweon,Yang Hoon Huh,Hak Rim Kim

Mitochondrion 34:115-126 PubMed28288917

2017

Cyclophilin D regulates lifespan and protein expression of aging markers in the brain of mice.

Applications

Unspecified application

Species

Unspecified reactive species

Viktoria Vereczki,Josef Mansour,Issa Pour-Ghaz,Ibolya Bodnar,Otto Pinter,Dora Zelena,Erzsebet Oszwald,Vera Adam-Vizi,Christos Chinopoulos

The European journal of neuroscience 36:3005-20 PubMed22805168

2012

Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Mariya Vasileva,Heinz Horstmann,Constanze Geumann,Daniel Gitler,Thomas Kuner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SYN2 antibody [EPR3277]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYN2 antibody [EPR3277] (AB76494)

Western blot - Anti-SYN2 antibody [EPR3277] (AB76494)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (12)

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