Rabbit Recombinant Monoclonal SYN2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 11 publications.
Images
![Western blot - Anti-SYN2 antibody [EPR3277] (AB76494), expandable thumbnail](https://content.abcam.com/products/images/syn2-antibody-epr3277-ab76494--western-blot-img58242.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SYN2 antibody [EPR3277] (AB76494)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYN2 antibody [EPR3277] (AB76494), expandable thumbnail](https://content.abcam.com/products/images/syn2-antibody-epr3277-ab76494--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72654.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYN2 antibody [EPR3277] (AB76494)")
Publications
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- Aging 13:23739-237562021Loganin substantially ameliorates molecular deficits, pathologies and cognitive impairment in a mouse model of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLulin Nie,Kaiwu He,Fengzhu Xie,Shifeng Xiao,Shupeng Li,Jia Xu,Kaiqin Zhang,Chen Yang,Li Zhou,Jianjun Liu,Liangyu Zou,Xifei YangPubMed 34689137
- Scientific reports 11:140012021The UL16 protein of HSV-1 promotes the metabolism of cell mitochondria by binding to ANT2 protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiyu Li,Shuting Liu,Zhenning Dai,Qian Zhang,Yichao Xu,Youyu Chen,Zhenyou Jiang,Wenhua Huang,Hanxiao SunPubMed 34234233
- iScience 24:1025042021Gene-environment interactions mediate stress susceptibility and resilience through the CaMKIIβ/TARPγ-8/AMPAR pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYusuke Sakai,Haiyan Li,Hiromichi Inaba,Yuki Funayama,Erina Ishimori,Ayako Kawatake-Kuno,Hirotaka Yamagata,Tomoe Seki,Teruyuki Hobara,Shin Nakagawa,Yoshifumi Watanabe,Susumu Tomita,Toshiya Murai,Shusaku UchidaPubMed 34113835
- Oxidative medicine and cellular longevity 2020:54084522020Proteomic Profile of Mouse Brain Aging Contributions to Mitochondrial Dysfunction, DNA Oxidative Damage, Loss of Neurotrophic Factor, and Synaptic and Ribosomal Proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingchao Li et. al.PubMed 32587661
- General physiology and biophysics 38:379-3882019Trafficking of synaptic vesicles is changed at the hypothalamus by exposure to an 835 MHz radiofrequency electromagnetic field.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJu Hwan Kim et. al.PubMed 31411574
- Scientific reports 9:12012019Decreased dopamine in striatum and difficult locomotor recovery from MPTP insult after exposure to radiofrequency electromagnetic fields.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJu Hwan Kim et. al.PubMed 30718744
- Food & function 9:1532-15442018Sustained consumption of cocoa-based dark chocolate enhances seizure-like events in the mouse hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Cicvaric et. al.PubMed 29431797
- PloS one 12:e01864162017Changes in numbers and size of synaptic vesicles of cortical neurons induced by exposure to 835 MHz radiofrequency-electromagnetic field.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJu Hwan Kim et. al.PubMed 29045446
- The European journal of neuroscience 36:3005-202012Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariya Vasileva,Heinz Horstmann,Constanze Geumann,Daniel Gitler,Thomas KunerPubMed 22805168
- Proceedings of the National Academy of Sciences of 108:21093-82011Neuronal differentiation by TAp73 is mediated by microRNA-34a regulation of synaptic protein targets.Applications:WBReactive species:Unspecified reactive speciesMassimiliano Agostini et. al.PubMed 22160687
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended | Predicted |
| Rat | Not recommended | Not recommended | Predicted | Not recommended | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 - 1/20000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. May play a role in noradrenaline secretion by sympathetic neurons (By similarity).
Alternative names
Synapsin-2, Synapsin II, SYN2
Recommended products
Rabbit Recombinant Monoclonal SYN2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 11 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3277
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-SYN2 antibody [EPR3277] (ab76494), expandable thumbnail](https://content.abcam.com/products/images/syn2-antibody-epr3277-ab76494--western-blot-img58242.jpg "Western blot - Anti-SYN2 antibody [EPR3277] (ab76494)")
Western blot - Anti-SYN2 antibody [EPR3277] (ab76494)
All lanes: Western blot - Anti-SYN2 antibody [EPR3277] (ab76494) at 1/20000 dilution
All lanes: Fetal brain tissue lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size: 63 kDa
Observed band size: 75 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYN2 antibody [EPR3277] (ab76494), expandable thumbnail](https://content.abcam.com/products/images/syn2-antibody-epr3277-ab76494--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72654.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYN2 antibody [EPR3277] (ab76494)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYN2 antibody [EPR3277] (ab76494)
Immunohistochemical analysis of paraffin-embedded human brain tissue using 1/100 ab76494
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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