AB18814

Anti-Synapsin I antibody

(4 Reviews)

(15 Publications)

Rabbit Polyclonal Synapsin I antibody. Suitable for WB and reacts with Rat, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human SYN1.

View Alternative Names

Synapsin-1, Brain protein 4.1, Synapsin I, SYN1

2 Images

Lab

Western blot - Anti-Synapsin I antibody (AB18814)

Blocking and dilution buffer : 5% NFDM/TBST.

Controls - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) and Anti-GAPDH antibody [EPR16891] (ab181602).

All lanes:

Western blot - Anti-Synapsin I antibody (ab18814) at 1/5000 dilution

Lane 1:

Human brain whole cell lysate - untreated at 10 µg

Lane 2:

Human brain whole cell lysate - treated with Alkaline Phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 74 kDa

Observed band size: 77 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-Synapsin I antibody (AB18814)

Blocking and dilution buffer : 5% NFDM/TBST.

Controls - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) and Anti-GAPDH antibody [EPR16891] (ab181602).

All lanes:

Western blot - Anti-Synapsin I antibody (ab18814) at 1/5000 dilution

Lane 1:

Rat brain whole cell lysate - untreated at 10 µg

Lane 2:

Rat brain whole cell lysate - treated with Alkaline Phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 74 kDa

Observed band size: 77 kDa

false

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat

Applications

applications

Immunogen

Synthetic Peptide within Human SYN1. The exact immunogen used to generate this antibody is proprietary information.

P17600

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 6008 Synapsin Antibody. 6008-100 is the same size as the 100 µg size of ab18814.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.02% Thimerosal (merthiolate) Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synapsin I also known as SYN1 plays an important role in synaptic function. It is a phosphoprotein with a molecular mass of approximately 78 kDa. Synapsin I is expressed mainly in the neurons of the central nervous system (CNS). It binds to synaptic vesicles and actin cytoskeleton which suggests that it functions in modulating neurotransmitter release at the presynaptic terminals. This modulation occurs as synapsin I undergoes phosphorylation which is critical for its activity.

Biological function summary

Synapsin I influences synaptic plasticity and is part of the synaptic vesicle trafficking complex. In its dephosphorylated state Synapsin I associates with synaptic vesicles anchoring them to the actin cytoskeleton. Upon phosphorylation Synapsin I changes conformation causing vesicles to mobilize. This activity supports the modulation of neurotransmitter release impacting learning and memory functions.

Pathways

Synapsin I participates significantly in the neurotransmitter release cycle and synaptic vesicle trafficking pathway. Protein kinase A (PKA) and Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulate its phosphorylation affecting how Synapsin I contributes to vesicle release. The phosphorylation of Synapsin I at sites such as serine 9 enables its interaction with other proteins like actin and spectrin facilitating vesicle movement.

Altered Synapsin I expression associates with neurological conditions like epilepsy and schizophrenia. In epilepsy dysregulation of Synapsin I phosphorylation processes can result in imbalanced neurotransmitter release potentially leading to seizures. Its connection to schizophrenia involves changes in synaptic plasticity which neurotransmitter systems such as dopamine and related proteins like alpha-synuclein also influence. Understanding these interactions can aid in developing therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neuronal phosphoprotein that coats synaptic vesicles, and binds to the cytoskeleton. Acts as a regulator of synaptic vesicles trafficking, involved in the control of neurotransmitter release at the pre-synaptic terminal (PubMed : 21441247, PubMed : 23406870). Also involved in the regulation of axon outgrowth and synaptogenesis (By similarity). The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level (By similarity).

See full target information SYN1

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Nature aging 5:1957-1969 PubMed40830655

2025

Targeting iron-associated protein Ftl1 in the brain of old mice improves age-related cognitive impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Remesal,Juliana Sucharov-Costa,Yuting Wu,Karishma J B Pratt,Gregor Bieri,Amber Philp,Mason Phan,Turan Aghayev,Charles W White,Elizabeth G Wheatley,Bende Zou,Brandon R Desousa,Julien Couthouis,Isha H Jian,Xinmin S Xie,Yi Lu,Jason C Maynard,Alma L Burlingame,Saul A Villeda

Theranostics 10:11794-11819 PubMed33052247

2020

Ultrasound with microbubbles improves memory, ameliorates pathology and modulates hippocampal proteomic changes in a triple transgenic mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Shen,Lingchen Hua,Chih-Kuang Yeh,Liming Shen,Ming Ying,Zaijun Zhang,Gongping Liu,Shupeng Li,Siping Chen,Xin Chen,Xifei Yang

Journal of neuroinflammation 17:77 PubMed32127019

2020

Supplement of microbiota-accessible carbohydrates prevents neuroinflammation and cognitive decline by improving the gut microbiota-brain axis in diet-induced obese mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hongli Shi,Qiao Wang,Mingxuan Zheng,Shanshan Hao,Jeremy S Lum,Xi Chen,Xu-Feng Huang,Yinghua Yu,Kuiyang Zheng

Current biology : CB 29:3359-3369.e4 PubMed31588002

2019

Neuronal O-GlcNAcylation Improves Cognitive Function in the Aged Mouse Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth G Wheatley,Eddy Albarran,Charles W White,Gregor Bieri,Cesar Sanchez-Diaz,Karishma Pratt,Cedric E Snethlage,Jun B Ding,Saul A Villeda

Cell death & disease 10:555 PubMed31324751

2019

Microglial TREM-1 receptor mediates neuroinflammatory injury via interaction with SYK in experimental ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Pengfei Xu,Xiaohao Zhang,Qian Liu,Yi Xie,Xiaolei Shi,Jingjing Chen,Yunzi Li,Hongquan Guo,Rui Sun,Ye Hong,Xinfeng Liu,Gelin Xu

Neurobiology of disease 127:527-544 PubMed30923003

2019

The UPR-PERK pathway is not a promising therapeutic target for mutant SOD1-induced ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Yulia Dzhashiashvili,Chase P Monckton,Harini S Shah,Rejani B Kunjamma,Brian Popko

Medical science monitor : international medical journal of experimental and clinical research 24:4982-4991 PubMed30018280

2018

Collapsin Response Mediator Protein-2 Ameliorates Sevoflurane-Mediated Neurocyte Injury by Targeting PI3K-mTOR-S6K Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxuan He,Jianfang Zhu

PloS one 13:e0197886 PubMed29864134

2018

Pleomorphic linkers as ubiquitous structural organizers of vesicles in axons.

Applications

Unspecified application

Species

Unspecified reactive species

Nikolas Schrod,Dimitri Vanhecke,Ulrike Laugks,Valentin Stein,Yoshiyuki Fukuda,Miroslava Schaffer,Wolfgang Baumeister,Vladan Lucic

Biomedicine & pharmacotherapy = Biomedecine & phar 101:391-398 PubMed29501042

2018

Clostridium difficile toxin B recombinant protein inhibits tumor growth and induces apoptosis through inhibiting Bcl-2 expression, triggering inflammatory responses and activating C-erbB-2 and Cox-2 expression in breast cancer mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Yunli Zhang,Yanming Li,Hongling Li,Wei Chen,Wenen Liu

Neuroscience 311:415-21 PubMed26518462

2015

An age-related reduction of brain TBPH/TDP-43 levels precedes the onset of locomotion defects in a Drosophila ALS model.

Applications

Unspecified application

Species

Unspecified reactive species

L Cragnaz,R Klima,L De Conti,G Romano,F Feiguin,E Buratti,M Baralle,F E Baralle

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Synapsin I antibody

Western blot - Anti-Synapsin I antibody (AB18814)

Western blot - Anti-Synapsin I antibody (AB18814)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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