Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)

Rabbit Recombinant Monoclonal Synapsin I antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P, IHC-Fr, Flow Cyt (Intra), mIHC and reacts with Rat, Mouse, Human samples. Cited in 1 publication.

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Images

Immunoprecipitation - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (AB274430), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	WB	IHC-P	IHC-Fr	Flow Cyt (Intra)	mIHC
Human	Expected	Expected	Tested	Tested	Expected	Expected	Tested
Mouse	Tested	Tested	Tested	Tested	Tested	Tested	Expected
Rat	Tested	Tested	Tested	Tested	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat, Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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4 products for Alternative Version

Target data

See full target information SYN1

Function

Neuronal phosphoprotein that coats synaptic vesicles, and binds to the cytoskeleton. Acts as a regulator of synaptic vesicles trafficking, involved in the control of neurotransmitter release at the pre-synaptic terminal (PubMed:21441247, PubMed:23406870). Also involved in the regulation of axon outgrowth and synaptogenesis (By similarity). The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level (By similarity).

Alternative names

Synapsin-1, Brain protein 4.1, Synapsin I, SYN1

Recommended products

Rabbit Recombinant Monoclonal Synapsin I antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P, IHC-Fr, Flow Cyt (Intra), mIHC and reacts with Rat, Mouse, Human samples. Cited in 1 publication.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR23531-50
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab274430 is the carrier-free version of Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Synapsin I also known as SYN1 plays an important role in synaptic function. It is a phosphoprotein with a molecular mass of approximately 78 kDa. Synapsin I is expressed mainly in the neurons of the central nervous system (CNS). It binds to synaptic vesicles and actin cytoskeleton which suggests that it functions in modulating neurotransmitter release at the presynaptic terminals. This modulation occurs as synapsin I undergoes phosphorylation which is critical for its activity.

Biological function summary

Synapsin I influences synaptic plasticity and is part of the synaptic vesicle trafficking complex. In its dephosphorylated state Synapsin I associates with synaptic vesicles anchoring them to the actin cytoskeleton. Upon phosphorylation Synapsin I changes conformation causing vesicles to mobilize. This activity supports the modulation of neurotransmitter release impacting learning and memory functions.

Pathways

Synapsin I participates significantly in the neurotransmitter release cycle and synaptic vesicle trafficking pathway. Protein kinase A (PKA) and Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulate its phosphorylation affecting how Synapsin I contributes to vesicle release. The phosphorylation of Synapsin I at sites such as serine 9 enables its interaction with other proteins like actin and spectrin facilitating vesicle movement.

Associated diseases and disorders

Altered Synapsin I expression associates with neurological conditions like epilepsy and schizophrenia. In epilepsy dysregulation of Synapsin I phosphorylation processes can result in imbalanced neurotransmitter release potentially leading to seizures. Its connection to schizophrenia involves changes in synaptic plasticity which neurotransmitter systems such as dopamine and related proteins like alpha-synuclein also influence. Understanding these interactions can aid in developing therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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19 product images

Immunoprecipitation - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Synapsin-1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
Lane 1: Mouse brain tissue lysate 10 ug
Lane 2: Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 IP in Mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 in mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
All lanes: Immunoprecipitation - Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349)
Predicted band size: 74 kDa
Observed band size: 70 kDa, 75 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neuron cells labelling Synapsin-1 with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain MAP2 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).
-ve control 1: Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution.
-ve control 2: Anti-MAP2 antibody [HM-2] ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human hippocampus tissue labeling Synapsin-1 with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining in human hippocampus (PMID: 22900032). The section was incubated with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse retina tissue labeling Synapsin-1 with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse retina is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Western blot - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Mouse lung (PMID: 9539796).
Exposure times: Lanes 1-3: 5.5 seconds; Lane 4: 3 minutes.
All lanes: Western blot - Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse lung tissue lysate at 20 µg
Lane 3: Rat brain tissue lysate at 20 µg
Lane 4: Rat spinal cord tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 74 kDa
Observed band size: 75 kDa
Flow Cytometry (Intracellular) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Mouse primary brain cells cells labelling Synapsin-1 with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/50 dilution (1ug) (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor^®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 15 seconds.
All lanes: Western blot - Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349) at 1/1000 dilution
All lanes: Human brain tissue lysate at 20 µg
Secondary
All lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 74 kDa
Observed band size: 70 kDa, 75 kDa
Western blot - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 15 seconds.
All lanes: Western blot - Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
Lane 3: Rat spinal cord tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 74 kDa
Observed band size: 70 kDa, 75 kDa
Immunoprecipitation - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Synapsin-1 was immunoprecipitated from 0.35 mg Rat brain tissue lysate with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
Lane 1: Rat brain tissue lysate 10 ug
Lane 2: Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 IP in Rat brain tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 in rat brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
All lanes: Immunoprecipitation - Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349)
Predicted band size: 74 kDa
Observed band size: 70 kDa, 75 kDa
Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Synapsin-1 with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling Synapsin-1 with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat retina tissue labeling Synapsin-1 with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on rat retina is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung tissue labeling Synapsin-1 with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution (Green). Negative control: no staining on mouse lung (PMID: 9539796) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung tissue labeling Synapsin-1 with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution (Green). Negative control: no staining on rat lung (PMID: 9539796) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neuron cells labelling Synapsin-1 with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in rat primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain MAP2 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).
-ve control 1: Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution.
-ve control 2: Anti-MAP2 antibody [HM-2] ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Synapsin-1 with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining in mouse hippocampus (PMID: 22900032). The section was incubated with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Synapsin-1 with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining in mouse pancreatic islets (PMID: 22334712). The section was incubated with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling Synapsin-1 with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining in rat retina (PMID: 2124189). The section was incubated with Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Multiplex immunohistochemistry - Anti-Synapsin I antibody [EPR23531-50] - BSA and Azide free (ab274430)
This data was developed using Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker ab254349, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human retina tissue labeling RPE65, Rhodopsin and Synapsin I with Anti-RPE65 antibody [EPR22579-44] ab231782 at 1/8000 dilution, Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free ab232934 at 1/8000 dilution and ab274430 at 1/1500 dilution followed by a ready to use Opal Polymer HRP Ms + Rb secondary antibody. Nuclear counter stain used was DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Panel A: merged staining of anti-Synapsin I (magenta; Opal™690), anti-RPE65 (green; Opal™520) and anti-Rhodopsin (red; Opal™570) on human retina.
Panel B: anti-RPE65 stained on pigmented layer.
Panel C: anti-Rhodopsin stained on rod photoreceptor cells.
Panel D: anti-Synapsin I stained on inner plexiform layer.

The section was incubated in three rounds of staining: in the order of ab274430, Anti-RPE65 antibody [EPR22579-44] ab231782, and Anti-Rhodopsin antibody [EPR21876] - BSA and Azide free ab232934 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

The section was incubated in three rounds of staining: in the order of Anti-Sialoadhesin/CD169 antibody [EPR27102-11] ab312840, Anti-CD3 epsilon antibody [SP7] ab16669, and Anti-CD20 antibody [SP32] - BSA and Azide free ab236434 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.