Anti-Synapsin I antibody - Synaptic Marker (ab64581) is a rabbit polyclonal antibody that is used to detect Synapsin I in Western Blot, IP. Suitable for Mouse, Rat samples.
- Over 110 publications
- Trusted since 2008
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- NPJ biofilms and microbiomes 9:992023Spinal cord injury-induced gut dysbiosis influences neurological recovery partly through short-chain fatty acids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingli Jing,Degang Yang,Fan Bai,Qiuying Wang,Chao Zhang,Yitong Yan,Zihan Li,Yan Li,Zhiguo Chen,Jianjun Li,Yan YuPubMed 38092763
- International journal of molecular sciences 24:2023Involvement of IL-1β-Mediated Necroptosis in Neurodevelopment Impairment after Neonatal Sepsis in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhimin Liao,Qing Zhu,Han HuangPubMed 37834141
- Neuroscience bulletin 39:1638-16542023Genetic Architecture and Functional Implications of the CSF-Contacting Nucleus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiyuan Song,Yumin Yuan,Lingling Xu,Jun Jiang,Ying Li,Yao Yan,Qing Li,Fang Zhou,Junli Cao,Licai ZhangPubMed 37405574
- JCI insight 8:2023MiR-431 attenuates synaptic plasticity and memory deficits in APPswe/PS1dE9 mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianwei Ge,Zhiwei Xue,Shu Shu,Linjie Yu,Ruomeng Qin,Wenyuan Tao,Pinyi Liu,Xiaohong Dong,Zhen Lan,Xinyu Bao,Lei Ye,Yun Xu,Xiaolei ZhuPubMed 37192007
- Acta neuropathologica communications 11:1022023DHCR24 reverses Alzheimer's disease-related pathology and cognitive impairment via increasing hippocampal cholesterol levels in 5xFAD mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Bin Zhang,Yue Huang,Xiao-Rou Guo,Meng-Qi Zhang,Xiang-Shan Yuan,Heng-Bing ZuPubMed 37344916
- International journal of molecular sciences 24:2023Early Alterations in Structural and Functional Properties in the Neuromuscular Junctions of Mutant FUS Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarat A Mukhamedyarov,Aydar N Khabibrakhmanov,Venera F Khuzakhmetova,Arthur R Giniatullin,Guzalia F Zakirjanova,Nikita V Zhilyakov,Kamilla A Mukhutdinova,Dmitry V Samigullin,Pavel N Grigoryev,Andrey V Zakharov,Andrey L Zefirov,Alexey M PetrovPubMed 37240370
- Frontiers in psychiatry 14:11923792023Hippocampal BAIAP2 prevents chronic mild stress-induced depression-like behaviors in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaling Fu,Xiangfei Guo,Rui Yang,Hao Feng,Xueyong Yin,Shuang Wang,Li Song,Xi Wang,Penghui Zhao,Sheng Wang,Yun Shi,Haishui ShiPubMed 37234209
- Nature neuroscience 25:1597-16072022Bassoon contributes to tau-seed propagation and neurotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPablo Martinez,Henika Patel,Yanwen You,Nur Jury,Abigail Perkins,Audrey Lee-Gosselin,Xavier Taylor,Yingjian You,Gonzalo Viana Di Prisco,Xiaoqing Huang,Sayan Dutta,Aruna B Wijeratne,Javier Redding-Ochoa,Syed Salman Shahid,Juan F Codocedo,Sehong Min,Gary E Landreth,Amber L Mosley,Yu-Chien Wu,David L McKinzie,Jean-Christophe Rochet,Jie Zhang,Brady K Atwood,Juan Troncoso,Cristian A Lasagna-ReevesPubMed 36344699
- Journal of neuroinflammation 19:2362022Injection of amyloid-β to lateral ventricle induces gut microbiota dysbiosis in association with inhibition of cholinergic anti-inflammatory pathways in Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Hang Qian,Xiao-Li Liu,Guang Chen,Sheng-di Chen,Hui-Dong TangPubMed 36171620
- Cell death & disease 13:7862022Crosstalk of Synapsin1 palmitoylation and phosphorylation controls the dynamicity of synaptic vesicles in neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeipei Yan,Huicong Liu,Tao Zhou,Pu Sun,Yilin Wang,Xibin Wang,Lin Zhang,Tian Wang,Jing Dong,Jiangli Zhu,Luxian Lv,Wenqiang Li,Shiqian Qi,Yinming Liang,Eryan KongPubMed 36097267
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Predicted | Predicted |
| Mouse | Tested | Tested |
| Rat | Expected | Tested |
| Goat | Predicted | Predicted |
| Sheep | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Goat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes Abcam recommends using 3-5% milk as the blocking agent. |
Species Rat | Dilution info 1 µg/mL | Notes Abcam recommends using 3-5% milk as the blocking agent. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Goat, Human | Dilution info - | Notes - |
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Target data
Function
Neuronal phosphoprotein that coats synaptic vesicles, and binds to the cytoskeleton. Acts as a regulator of synaptic vesicles trafficking, involved in the control of neurotransmitter release at the pre-synaptic terminal (By similarity). Also involved in the regulation of axon outgrowth and synaptogenesis (PubMed:7568107). The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxide functions at a presynaptic level (By similarity).
Alternative names
Syn-1, Syn1, Synapsin-1, Synapsin I
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Anti-Synapsin I antibody - Synaptic Marker (ab64581) is a rabbit polyclonal antibody that is used to detect Synapsin I in Western Blot, IP. Suitable for Mouse, Rat samples.
- Over 110 publications
- Trusted since 2008
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Replenishment batches of our polyclonal antibody, ab64581 are tested in WB. Previous batches were additionally validated in IP. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab254349.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Synapsin I antibody - Synaptic Marker (ab64581) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Mouse, Rat samples.
What is the molecular weight of Synapsin I?
Anti-Synapsin I - Synaptic Marker (ab64581) specifically detects a band for Synapsin I (UniProt: P09951) at a molecular weight of 74kDa.
Trusted by the scientific community
Anti-Synapsin I - Synaptic Marker (ab64581) was first used in a scientific publication in 2008 and has been cited over 110 times in peer-reviewed journals.
Reviewed by scientists
Anti-Synapsin I - Synaptic Marker (ab64581) has over 20 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Synapsin I also known as SYN1 plays an important role in synaptic function. It is a phosphoprotein with a molecular mass of approximately 78 kDa. Synapsin I is expressed mainly in the neurons of the central nervous system (CNS). It binds to synaptic vesicles and actin cytoskeleton which suggests that it functions in modulating neurotransmitter release at the presynaptic terminals. This modulation occurs as synapsin I undergoes phosphorylation which is critical for its activity.
- Biological function summary
Synapsin I influences synaptic plasticity and is part of the synaptic vesicle trafficking complex. In its dephosphorylated state Synapsin I associates with synaptic vesicles anchoring them to the actin cytoskeleton. Upon phosphorylation Synapsin I changes conformation causing vesicles to mobilize. This activity supports the modulation of neurotransmitter release impacting learning and memory functions.
- Pathways
Synapsin I participates significantly in the neurotransmitter release cycle and synaptic vesicle trafficking pathway. Protein kinase A (PKA) and Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulate its phosphorylation affecting how Synapsin I contributes to vesicle release. The phosphorylation of Synapsin I at sites such as serine 9 enables its interaction with other proteins like actin and spectrin facilitating vesicle movement.
- Associated diseases and disorders
Altered Synapsin I expression associates with neurological conditions like epilepsy and schizophrenia. In epilepsy dysregulation of Synapsin I phosphorylation processes can result in imbalanced neurotransmitter release potentially leading to seizures. Its connection to schizophrenia involves changes in synaptic plasticity which neurotransmitter systems such as dopamine and related proteins like alpha-synuclein also influence. Understanding these interactions can aid in developing therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
The immunogen for ab64581 is predicted to recognise both the 70 kDa and 74 kDa isoforms of Synapsin I. Synapsin I is also known to contain a number of potential phosphorylation and glycosylation sites (SwissProt data).
All lanes: Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581) at 1 µg/mL
Lane 1: Spinal Cord (Mouse) Tissue Lysate at 10 µg
Lane 2: Brain (Mouse) Tissue Lysate at 10 µg
Lane 3: Brain (Rat) Tissue Lysate - normal tissue at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 70 kDa, 74 kDa
This image is courtesy of an anonymous customer reviewWestern blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
Blocked with 5% milk for 1 hour at 25°C.
Sample was incubated with primary antibody for 18 hours at 4°C in TBS + 0.1% Tween.
All lanes: Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581) at 1/5000 dilution
All lanes: Rat whole brain tissue lysate at 15 µg
SecondaryAll lanes: Donkey anti-Rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 80 kDa, 85 kDa
Exposure time: 15s
Immunoprecipitation - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
Synapsin I - Synaptic Marker was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Synapsin I - Synaptic Marker and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64581.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 80kDa: Synapsin I - Synaptic Marker contains a number of potential glycosylation sites (SwissProt) which may explain the higher migrating band.All lanes: Immunoprecipitation - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
Predicted band size: 74 kDa
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