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Rabbit Polyclonal Synapsin I antibody. Synapse marker. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 110 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Predicted
Predicted
Mouse
Tested
Tested
Rat
Expected
Tested
Goat
Predicted
Predicted
Sheep
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Sheep, Goat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1 µg/mL

Notes

Abcam recommends using 3-5% milk as the blocking agent.

Species

Rat

Dilution info

1 µg/mL

Notes

Abcam recommends using 3-5% milk as the blocking agent.

Predicted
Predicted

Species

Sheep, Goat, Human

Dilution info

-

Notes

-

Target data

Function

Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. Regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxide functions at a presynaptic level.

Alternative names

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Rabbit Polyclonal Synapsin I antibody. Synapse marker. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 110 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Specificity

Replenishment batches of our polyclonal antibody, ab64581 are tested in WB. Previous batches were additionally validated in IP. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab254349.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

Synapsin I influences synaptic plasticity and is part of the synaptic vesicle trafficking complex. In its dephosphorylated state Synapsin I associates with synaptic vesicles anchoring them to the actin cytoskeleton. Upon phosphorylation Synapsin I changes conformation causing vesicles to mobilize. This activity supports the modulation of neurotransmitter release impacting learning and memory functions.

Activity summary

Synapsin I also known as SYN1 plays an important role in synaptic function. It is a phosphoprotein with a molecular mass of approximately 78 kDa. Synapsin I is expressed mainly in the neurons of the central nervous system (CNS). It binds to synaptic vesicles and actin cytoskeleton which suggests that it functions in modulating neurotransmitter release at the presynaptic terminals. This modulation occurs as synapsin I undergoes phosphorylation which is critical for its activity.

Pathways

Synapsin I participates significantly in the neurotransmitter release cycle and synaptic vesicle trafficking pathway. Protein kinase A (PKA) and Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulate its phosphorylation affecting how Synapsin I contributes to vesicle release. The phosphorylation of Synapsin I at sites such as serine 9 enables its interaction with other proteins like actin and spectrin facilitating vesicle movement.

Associated diseases and disorders

Altered Synapsin I expression associates with neurological conditions like epilepsy and schizophrenia. In epilepsy dysregulation of Synapsin I phosphorylation processes can result in imbalanced neurotransmitter release potentially leading to seizures. Its connection to schizophrenia involves changes in synaptic plasticity which neurotransmitter systems such as dopamine and related proteins like alpha-synuclein also influence. Understanding these interactions can aid in developing therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)

    Blocked with 5% milk for 1 hour at 25°C.

    Sample was incubated with primary antibody for 18 hours at 4°C in TBS + 0.1% Tween.

    All lanes: Western blot - Anti-Synapsin I antibody - Synaptic Marker (AB64581) at 1/5000 dilution

    All lanes: Rat whole brain tissue lysate at 15 µg

    Secondary

    All lanes: Donkey anti-Rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 74 kDa

    Observed band size: 80 kDa, 85 kDa

    Exposure time: 15s

  • Immunoprecipitation - Anti-Synapsin I antibody - Synaptic Marker (ab64581), expandable thumbnail

    Immunoprecipitation - Anti-Synapsin I antibody - Synaptic Marker (ab64581)

    Synapsin I - Synaptic Marker was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Synapsin I - Synaptic Marker and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64581.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 80kDa: Synapsin I - Synaptic Marker contains a number of potential glycosylation sites (SwissProt) which may explain the higher migrating band.

    All lanes: Immunoprecipitation - Anti-Synapsin I antibody - Synaptic Marker (AB64581)

    Predicted band size: 74 kDa

  • Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581), expandable thumbnail

    Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)

    The immunogen for ab64581 is predicted to recognise both the 70 kDa and 74 kDa isoforms of Synapsin I. Synapsin I is also known to contain a number of potential phosphorylation and glycosylation sites (SwissProt data).

    All lanes: Western blot - Anti-Synapsin I antibody - Synaptic Marker (AB64581) at 1 µg/mL

    Lane 1: Spinal Cord (Mouse) Tissue Lysate at 10 µg

    Lane 2: Brain (Mouse) Tissue Lysate at 10 µg

    Lane 3: Brain (Rat) Tissue Lysate - normal tissue at 10 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 74 kDa

    Observed band size: 70 kDa, 74 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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