AB32532

Anti-Synapsin I (phospho S553) antibody [E377]

RabMAb
Recombinant
20ul selling size
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(14 Publications)

Rabbit Recombinant Monoclonal Synapsin I phospho S553 antibody. Suitable for Dot, WB and reacts with Human, Mouse, Rat samples. Cited in 14 publications.

View Alternative Names

Synapsin-1, Brain protein 4.1, Synapsin I, SYN1

5 Images

Lab

Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (AB32532)

All lanes:

Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution

Lane 1:

Untreated Human fetal brain lysates at 15 µg

Lane 2:

Human fetal brain lysates treated with alkaline phosphatase at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 74 kDa

Observed band size: 77 kDa

false

Unknown

Dot Blot - Anti-Synapsin I (phospho S553) antibody [E377] (AB32532)

Dot Blot analysis on human immunogen phospho-peptide (A) and non-phospho peptide (B) using ab32532 at a dilution of 1/500

Unknown

Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (AB32532)

Blocking buffer : 2% BSA/TBST

Dilution buffer : 2% BSA/TBST

All lanes:

Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution

Lane 1:

Untreated rat brain whole cell lysates at 10 µg

Lane 2:

Rat brain whole cell lysates, treated with alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 74 kDa

Observed band size: 77 kDa

false

Exposure time: 1s

Unknown

Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (AB32532)

Blocking buffer : 2% BSA/TBST

Dilution buffer : 2% BSA/TBST

All lanes:

Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/200 dilution

Lane 1:

Untreated mouse brain whole cell lysate at 10 µg

Lane 2:

Mouse brain whole cell lysates, treated with alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 74 kDa

Observed band size: 77 kDa

false

Exposure time: 3min

Unknown

Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (AB32532)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution

Lane 1:

Untreated mouse brain whole cell lysate at 10 µg

Lane 2:

Mouse brain whole cell lysate, treated with alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 74 kDa

Observed band size: 77 kDa

false

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E377

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects Synapsin phosphorylated on Serine 553

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/500", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synapsin I also known as SYN1 plays an important role in synaptic function. It is a phosphoprotein with a molecular mass of approximately 78 kDa. Synapsin I is expressed mainly in the neurons of the central nervous system (CNS). It binds to synaptic vesicles and actin cytoskeleton which suggests that it functions in modulating neurotransmitter release at the presynaptic terminals. This modulation occurs as synapsin I undergoes phosphorylation which is critical for its activity.

Biological function summary

Synapsin I influences synaptic plasticity and is part of the synaptic vesicle trafficking complex. In its dephosphorylated state Synapsin I associates with synaptic vesicles anchoring them to the actin cytoskeleton. Upon phosphorylation Synapsin I changes conformation causing vesicles to mobilize. This activity supports the modulation of neurotransmitter release impacting learning and memory functions.

Pathways

Synapsin I participates significantly in the neurotransmitter release cycle and synaptic vesicle trafficking pathway. Protein kinase A (PKA) and Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulate its phosphorylation affecting how Synapsin I contributes to vesicle release. The phosphorylation of Synapsin I at sites such as serine 9 enables its interaction with other proteins like actin and spectrin facilitating vesicle movement.

Altered Synapsin I expression associates with neurological conditions like epilepsy and schizophrenia. In epilepsy dysregulation of Synapsin I phosphorylation processes can result in imbalanced neurotransmitter release potentially leading to seizures. Its connection to schizophrenia involves changes in synaptic plasticity which neurotransmitter systems such as dopamine and related proteins like alpha-synuclein also influence. Understanding these interactions can aid in developing therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neuronal phosphoprotein that coats synaptic vesicles, and binds to the cytoskeleton. Acts as a regulator of synaptic vesicles trafficking, involved in the control of neurotransmitter release at the pre-synaptic terminal (PubMed : 21441247, PubMed : 23406870). Also involved in the regulation of axon outgrowth and synaptogenesis (By similarity). The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level (By similarity).

See full target information SYN1 phospho S553

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Translational psychiatry 14:47 PubMed38253622

2024

Hydroxynorketamine, but not ketamine, acts via α7 nicotinic acetylcholine receptor to control presynaptic function and gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Debarpan Guhathakurta,Aneta Petrušková,Enes Yağız Akdaş,Bartomeu Perelló-Amorós,Renato Frischknecht,Daniela Anni,Eva-Maria Weiss,Martin Walter,Anna Fejtová

Cell death & disease 13:786 PubMed36097267

2022

Crosstalk of Synapsin1 palmitoylation and phosphorylation controls the dynamicity of synaptic vesicles in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Peipei Yan,Huicong Liu,Tao Zhou,Pu Sun,Yilin Wang,Xibin Wang,Lin Zhang,Tian Wang,Jing Dong,Jiangli Zhu,Luxian Lv,Wenqiang Li,Shiqian Qi,Yinming Liang,Eryan Kong

Skeletal muscle 12:19 PubMed35906707

2022

Oxidative stress-induced premature senescence and aggravated denervated skeletal muscular atrophy by regulating progerin-p53 interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoxian Xiang,Zongqi You,Xinying Huang,Junxi Dai,Junpeng Zhang,Shuqi Nie,Lei Xu,Junjian Jiang,Jianguang Xu

EMBO reports 23:e53659 PubMed35766170

2022

Bassoon controls synaptic vesicle release via regulation of presynaptic phosphorylation and cAMP.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Montenegro-Venegas,Debarpan Guhathakurta,Eneko Pina-Fernandez,Maria Andres-Alonso,Florian Plattner,Eckart D Gundelfinger,Anna Fejtova

Molecular brain 15:35 PubMed35461284

2022

Roles of Rufy3 in experimental subarachnoid hemorrhage-induced early brain injury via accelerating neuronal axon repair and synaptic plasticity.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Jianguo Xu,Wanchun You,Haitao Shen,Xiang Li,Zhengquan Yu,Haiying Li,Gang Chen

Nature communications 13:1824 PubMed35383160

2022

Multiomic profiling of the acute stress response in the mouse hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Lukas M von Ziegler,Amalia Floriou-Servou,Rebecca Waag,Rebecca R Das Gupta,Oliver Sturman,Katharina Gapp,Christina A Maat,Tobias Kockmann,Han-Yu Lin,Sian N Duss,Mattia Privitera,Laura Hinte,Ferdinand von Meyenn,Hanns U Zeilhofer,Pierre-Luc Germain,Johannes Bohacek

Cell death & disease 13:292 PubMed35365601

2022

Loss of neurodevelopmental-associated miR-592 impairs neurogenesis and causes social interaction deficits.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Fu,Yang Zhou,Yuan-Lin Zhang,Bo Zhao,Xing-Liao Zhang,Wan-Ting Zhang,Yi-Jun Lu,Aiping Lu,Jun Zhang,Jing Zhang

Medical oncology (Northwood, London, England) 39:15 PubMed34817735

2021

ELK1-induced up-regulation of KIF26B promotes cell cycle progression in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

SuXia Wang,Hui Zhang,HaiTing Liu,XiangYu Guo,RanRan Ma,WenJie Zhu,P Gao

Molecular psychiatry 26:7560-7580 PubMed34433918

2021

Cortical organoids model early brain development disrupted by 16p11.2 copy number variants in autism.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge Urresti,Pan Zhang,Patricia Moran-Losada,Nam-Kyung Yu,Priscilla D Negraes,Cleber A Trujillo,Danny Antaki,Megha Amar,Kevin Chau,Akula Bala Pramod,Jolene Diedrich,Leon Tejwani,Sarah Romero,Jonathan Sebat,John R Yates Iii,Alysson R Muotri,Lilia M Iakoucheva

Cellular and molecular life sciences : CMLS 78:4973-4992 PubMed33864480

2021

Aβ1-16 controls synaptic vesicle pools at excitatory synapses via cholinergic modulation of synapsin phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Anni,Eva-Maria Weiss,Debarpan Guhathakurta,Yagiz Enes Akdas,Julia Klueva,Stefanie Zeitler,Maria Andres-Alonso,Tobias Huth,Anna Fejtova

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Synapsin I (phospho S553) antibody [E377]

Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (AB32532)

Dot Blot - Anti-Synapsin I (phospho S553) antibody [E377] (AB32532)

Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (AB32532)

Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (AB32532)

Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (AB32532)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

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